Regulation of inducible nitric oxide synthase expression in bovine ovarian granulosa cells

Zamberlam, Gustavo; Portela, Valério Marques; Oliveira, João Francisco Coelho de; Gonçalves, Paulo Bayard Dias; Price, Christopher A.

doi:10.1016/j.mce.2011.01.013

Cited by 25 publications

(14 citation statements)

References 48 publications

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“…Results are expressed relative to DAPI. Cleaved caspase-3 abundance was consistent with flow cytometric assessment of apoptosis in granulosa cells in a previous study [35].…”

Section: Assessment Of Apoptosissupporting

confidence: 88%

“…Real-time PCR was conducted in an ABI Prism 7300 instrument (Applied Biosystems, Foster City, CA) with Power SYBR Green PCR Master Mix (Applied Biosystems) and bovinespecific primers for amplifying histone H2AFZ [15], FGF18 [15], CYP19A1 [32], GADD45B [33], BAX [34], FASLG [35], mouse double minute homolog-2 (MDM2; forward 5'-TCAGGCAGGCGAGAGTGATA-3', reverse 5'-GGGAGGATTCATTTCATTGCA-3'), MDM2 binding protein (MTBP; forward 5'-GGTTCTTTGCAGTTCAGGCAAT-3', reverse 5'-TGTCATCTTTCTCTGCACCAAAA-3') and BBC3 (forward 5'-GCTATACGAGTGGCGGAGAC-3', reverse 5'-AAGGGCAGGAGTCCCATGAT-3') cDNA. Primers for BBC3 were designed to encompass the BH3 domain and thus measure transcripts that give rise to biologically active forms of the protein [36], and the primers of each gene were located either side of an exon boundary.…”

Section: Real-time Rt-pcrmentioning

confidence: 99%

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The Role of Fibroblast Growth Factor-18 in Follicular Atresia in Cattle1

Portela

Dirandeh

Guerrero-Netro

et al. 2015

Biology of Reproduction

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Although the various members of the fibroblast growth factor (FGF) family are generally mitotic, one member, FGF18, has been shown to increase the rate of apoptosis of ovarian granulosa cells. In the present study, we first determined whether granulosa cells express FGF18 and we then explored the mechanism through which FGF18 increases apoptosis in vitro. Under culture conditions that favored estradiol secretion and CYP19A1 expression, granulosa FGF18 mRNA levels were barely detectable; however, withdrawing gonadotropic support (follicle-stimulating hormone or insulin-like growth factor 1) reduced levels of CYP19A1 mRNA and increased abundance of mRNA encoding the death ligand FASLG and FGF18. Addition of FGF18, but not FGF2, FGF10, or EGF, increased the proportion of apoptotic cells and frequency of caspase 3 activation, and these effects were abrogated by coculture with estradiol. Addition of FGF18 decreased abundance of mRNA encoding the antiapoptotic proteins GADD45B and MDM2, and increased that encoding the proapoptotic protein BBC3; these effects were reversed by coculture with estradiol. The physiological relevance of FGF18 was determined using an in vivo model: injection of FGF18 directly into growing bovine dominant follicles caused cessation of follicle growth by 24 h after injection. Collectively, these data demonstrate that FGF18 is proapoptotic in vivo and may act through a mechanism involving the BBC3-MDM2 pathway.

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“…Results are expressed relative to DAPI. Cleaved caspase-3 abundance was consistent with flow cytometric assessment of apoptosis in granulosa cells in a previous study [35].…”

Section: Assessment Of Apoptosissupporting

confidence: 88%

Section: Real-time Rt-pcrmentioning

confidence: 99%

The Role of Fibroblast Growth Factor-18 in Follicular Atresia in Cattle1

Portela

Dirandeh

Guerrero-Netro

et al. 2015

Biology of Reproduction

Self Cite

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“…Similarly, RNS influence ovarian function and the development at puberty [17]. Several studies suggest that NO alters reproductive physiology [17][18][19] by regulating pulsatile hypothalamic GnRH secretion, thereby also mediating sexual behavior in female rats [18], and indirectly inhibiting human granulosa-luteal cell steroidogenesis [19].…”

Section: Reactive Species and Melatoninmentioning

confidence: 99%

Essential actions of melatonin in protecting the ovary from oxidative damage

Cruz¹,

Leal²,

Cruz

et al. 2014

Theriogenology

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“…Data supporting NO antiapoptotic effects have been documented by Zamberlam et al (2011), who hypothesized that increased NO levels in the bovine dominant follicle would inhibit FasL-mediated apoptosis.…”

Section: Regulation Of No Productionmentioning

confidence: 90%

“…In addition, Moonmanee et al (2013) pointed out a relationship among eNOS expression, vascularization, and mitotic activity in the first follicular wave, thus suggesting a role for eNOS in selection of nonovulatory dominant follicles. Herath et al (2007) did not detect iNOS mRNA in ruminant granulosa cells, while Zamberlam et al (2011) documented the expression of iNOS in the same cells and its regulation by FSH and insulin-like growth factor 1, probably mediated by estradiol. These observations would support the hypothesis that, in this species, endogenous NO production could be involved in follicle selection.…”

Section: Regulation Of No Productionmentioning

confidence: 92%

Nitric oxide in follicle development and oocyte competence

Basini

Grasselli

2015

Reproduction

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Apart from its well-known role in regulating endothelial function, in mammals, nitric oxide (NO) is an important signaling molecule involved in many processes, regulating different biological functions. It has been demonstrated that NO plays a role in the physiology of the reproductive system, where it acts in controlling the activity of reproductive organs in both sexes. In the female of several animal species, experimental data suggest the presence of an intraovarian NO-generating system, which could be involved in the control of follicular development. The role of NO in regulating follicular atresia by apoptosis is still controversial, as a dual action depending mostly on its concentration has been documented. NO also displays positive effects on follicle development and selection related to angiogenic events and it could also play a modulatory role in steroidogenesis in ovarian cells. Both in monovulatory and poliovulatory species, the increase in PGE 2 production induced by NO via a stimulatory effect on COX-2 activity appears to be a common ovulatory mechanism. Considerable evidence also exists to support an involvement of the NO/NO synthase system in the control of meiotic maturation of cumulus-oocyte complexes.Reproduction (2015) 150 R1-R9

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Regulation of inducible nitric oxide synthase expression in bovine ovarian granulosa cells

Cited by 25 publications

References 48 publications

The Role of Fibroblast Growth Factor-18 in Follicular Atresia in Cattle1

The Role of Fibroblast Growth Factor-18 in Follicular Atresia in Cattle1

Essential actions of melatonin in protecting the ovary from oxidative damage

Nitric oxide in follicle development and oocyte competence

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