Regulation of Inducible and Tissue-Specific Gene Expression

Maniatis, Tom; Goodbourn, Stephen; Fischer, Janice A.

doi:10.1126/science.3296191

Cited by 1,582 publications

(685 citation statements)

References 147 publications

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“…Out of the conserved sequence set of typical eukaryotic promoters consisting of UPEs and a TATA-box flanked by GC-rich stretches [31] only the TATA-box element is present in 3 of the 7 genes. The other 4 genes contain at corresponding positions TATA-box-like sequences with GC-flanks on either side.…”

Section: Discussionmentioning

confidence: 99%

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Regulatory structures of gene expression, DNA-replication and DNA-rearrangement in macronuclear genes of Stylonychia lemnae, a hypotrichous ciliate

Helftenbein

Conzelmann

Becker

et al. 1989

European Journal of Protistology

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Section: Discussionmentioning

confidence: 99%

“…Two types of nucleotide sequences involved in regulation of transcription initiation have been identified: promoters and enhancers [31]. Promoters are necessary for accurate and efficient initiation of transcription and are located within the 100 bp immediately upstream of the transcription start sites.…”

Section: Introductionmentioning

confidence: 99%

Regulatory structures of gene expression, DNA-replication and DNA-rearrangement in macronuclear genes of Stylonychia lemnae, a hypotrichous ciliate

Helftenbein

Conzelmann

Becker

et al. 1989

European Journal of Protistology

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“…Presumably these elements are important in regulating the efficiency and accuracy of transcription initiation. In contrast to the conserved position of such basal promoter sequences, enhancer elements can function at a considerable distance from the promoter and can be located 5′ or 3′ of the transcription start site (for review see Maniatis et al, 1987). Before searching for such elements via sequence comparisons, we narrowed down the region required for Mhc expression by examining transgenic lines containing Mhc sequences linked to a reporter gene.…”

Section: The 5′ End Of Mhc Is Sufficient For Muscle-specific Expressimentioning

confidence: 99%

Transcriptional regulation of the Drosophila melanogaster muscle myosin heavy-chain gene

Hess

Singer

Trinh

et al. 2007

Gene Expression Patterns

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We show that a 2.6 kb fragment of the muscle myosin heavy-chain gene (Mhc) of Drosophila melanogaster (containing 458 base pairs of upstream sequence, the first exon, the first intron and the beginning of the second exon) drives expression in all muscles. Comparison of the minimal promoter to Mhc genes of ten Drosophila species identified putative regulatory elements in the upstream region and in the first intron. The first intron is required for expression in four small cells of the tergal depressor of the trochanter (jump) muscle and in the indirect flight muscle. The 3′ end of this intron is important for Mhc transcription in embryonic body wall muscle and contains AT-rich elements that are protected from DNase I digestion by nuclear proteins of Drosophila embryos. Sequences responsible for expression in embryonic, adult body wall and adult head muscles are present both within and outside the intron. Elements important for expression in leg muscles and in the large cells of the jump muscle flank the intron. We conclude that multiple transcriptional regulatory elements are responsible for Mhc expression in specific sets of Drosophila muscles. KeywordsDrosophila melanogaster; muscle; myosin heavy chain; transcription; enhancer; gene regulation Drosophila melanogaster is unusual in having a single muscle myosin heavy-chain gene (Mhc) (Bernstein et al., 1983;Rozek and Davidson, 1983), rather than a Mhc multigene family (see Emerson and Bernstein, 1987 for review). Alternative splicing of the primary transcripts from this gene yields multiple isoforms of the protein (Collier et al., 1990;George et al., 1989;Hastings and Emerson, 1991;Kazzaz and Rozek, 1989;Kronert et al., 1991;Zhang and Bernstein, 2001). Drosophila Mhc is expressed in all muscles at embryonic, larval, pupal and adult stages. Thus several stage-or muscle-specific enhancer elements may regulate its transcription.Transcriptional regulatory regions for a number of Drosophila muscle genes have been mapped, resulting in the identification of both unique and shared cis-acting elements (Arredondo et al., 2001;Kelly et al., 2002;Marin et al., 2004;Mas et al., 2004;Meredith and Storti, 1993). The latter includes E-boxes that bind helix-loop-helix transcription factors and MEF2 sites that bind MEF2 protein (Bour et al., 1995; Lilly et al., 1995). E-boxes, MEF2 sites and their binding factors positively regulate vertebrate muscle gene expression as well (see Molkentin and Olson, 1996 for review).* Corresponding author. Tel.: +1-619-594-5629; fax: +1-619-594-5676; E-mail address: sbernst@sunstroke.sdsu.edu. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal dis...

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“…The region -120 involves hexanucleotide GGGCCG, which binds factor Spl [79]. The putative regulatory region of the a2 gene contains many oligo-G blocks 1421.…”

Section: On Regulation Of Expression Of Genes For Na+k+-atpasementioning

confidence: 99%

Advances in Na⁺,K⁺‐ATPase studies: from protein to gene and back to protein

Broude¹,

Modyanov²,

Monastyrskaya³

1989

FEBS Letters

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Complete primary stators of both submit of Na',K+-ATPase from various sources have been ~~biisbed by a ~mbination of the methods for molecular cloning and protein chemistry. The gene family homologo~ to the a-subunit cDNA of animal Na+,K*-ATPases has been found in the human genome. Some genes of this family encode the known isoforms (aI and aI1) of the Na+,K+-ATPase catalytic subunit. The proteins coded by other genes can be either new isoforms of the Na+,K+-ATPase catalytic subunit or other ion-transporting ATPases. Expression of the genes of this family proceeds in a tissue-specific manner and changes during the postnatal development and neoplastic transformation. The complete exon-intron structure of one of the genes of this family has been established. This gene codes for the form of the catalytic subunit, the existence of which has been unknown. Apparently, all the genes of the discovered family have a similar intron-exon structure. There is certain correlation between the gene structure and the proposed domain a~angement of the a-subunit. The results obtained have become the basis for the experiments which prove the existence of the earlier unknown afI1 isoform of the Na+,K+-ATPase catalytic subunit and have made possible the study of its function.

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Regulation of Inducible and Tissue-Specific Gene Expression

Cited by 1,582 publications

References 147 publications

Regulatory structures of gene expression, DNA-replication and DNA-rearrangement in macronuclear genes of Stylonychia lemnae, a hypotrichous ciliate

Regulatory structures of gene expression, DNA-replication and DNA-rearrangement in macronuclear genes of Stylonychia lemnae, a hypotrichous ciliate

Transcriptional regulation of the Drosophila melanogaster muscle myosin heavy-chain gene

Advances in Na⁺,K⁺‐ATPase studies: from protein to gene and back to protein

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Regulation of Inducible and Tissue-Specific Gene Expression

Cited by 1,582 publications

References 147 publications

Regulatory structures of gene expression, DNA-replication and DNA-rearrangement in macronuclear genes of Stylonychia lemnae, a hypotrichous ciliate

Regulatory structures of gene expression, DNA-replication and DNA-rearrangement in macronuclear genes of Stylonychia lemnae, a hypotrichous ciliate

Transcriptional regulation of the Drosophila melanogaster muscle myosin heavy-chain gene

Advances in Na+,K+‐ATPase studies: from protein to gene and back to protein

Contact Info

Product

Resources

About

Advances in Na⁺,K⁺‐ATPase studies: from protein to gene and back to protein