Regulation of in vitro and in vivo transcription of early-region IV of adenovirus type 5 by multiple cis-acting elements.

Hanaka, Satoko; Nishigaki, Toshinori; Sharp, Phillip A.; Handa, Hiroshi

doi:10.1128/mcb.7.7.2578

Cited by 35 publications

(46 citation statements)

References 39 publications

(61 reference statements)

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“…25 bp upstream from the normal initiation site. The extra transcripts from the ID-20 promoter were also observed in in vitro transcription with HeLa cell extracts (14). DISCUSSION We constructed a set of mutations in the adenovirus EIV promoter and identified several cis-acting regulatory dermains.…”

Section: Stimulation Of Peiv-dhfr Fusion Genes By the Ela Regionmentioning

confidence: 99%

“…The EIV promoter of adenovirus is active in human cells in the absence of the Ela gene products (9,14). To characterize the ability of the Ela gene products to stimulate this activity, we mnade use of a stable cotransfection protocol that was previously used to characterize regulation of the adenovirus ElI promoter (23).…”

Section: Stimulation Of Peiv-dhfr Fusion Genes By the Ela Regionmentioning

confidence: 99%

“…The fourth, most upstream region is located between -260 and -307 and contains nuclear factor I-binding sites (30). These domains are also necessary for full expression in vivo (14).…”

mentioning

confidence: 99%

“…We constructed a series of mutations in the EIV promoter and identified several cis-acting domains necessary for accurate and efficient transcription of this promoter in vitro (14,16). Four domains were identified.…”

mentioning

confidence: 99%

“…1. To construct pEIVWT-, E-, S-, ID-DHFR plasmids, pSmaIL (16) contains the EII promoter region of Ad2 fused cDNA, as described previously (14). pEIVWT (27), electrophoresed on 1.0% agarose gels, and transferred to nitrocellulose.…”

mentioning

confidence: 99%

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A specific domain of the adenovirus EIV promoter is necessary to maintain susceptibility of the integrated promoter to EIA transactivation.

Nishigaki¹,

Hanaka²,

Kingston³

et al. 1988

Mol. Cell. Biol.

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We constructed a series of mutations that delete sequences in the promoter region of the early-region IV (EIV) promoter of adenovirus type 5. We fused these promoter mutations to the coding sequences of either the chloramphenicol acetyltransferase or the dihydrofolate reductase (DHFR) gene and tested the ability of a cotransfected EIa gene to stimulate EIV expression. All of the mutations tested were stimulated in these assays, implying that no specific sequence is required for stimulation. Two mutant promoters, deleted for either the TATA box or the region residing between -39 and -177 upstream from the cap site of EIV mRNA, did show a reduced level of stimulation by the EIa products. To assess the effects of the EIA gene products on expression from an EIV promoter integrated into the chromosome, we isolated CHO cell lines containing EIV-DHFR chimeric genes. After introduction of the EIa gene with a second selectable marker, expression from all mutant EIV-DHFR genes was increased. Surprisingly, one mutant promoter, deleted for sequences between -39 and -177, lost the ability to respond to the EIa region on passage of cells, although deletions in any part of the region still retained this ability. These results demonstrate that multiple elements residing between -39 and -177 in the EIV promoter are necessary to maintain susceptibility of the integrated promoter to regulation.

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Section: Stimulation Of Peiv-dhfr Fusion Genes By the Ela Regionmentioning

confidence: 99%

Section: Stimulation Of Peiv-dhfr Fusion Genes By the Ela Regionmentioning

confidence: 99%

“…The fourth, most upstream region is located between -260 and -307 and contains nuclear factor I-binding sites (30). These domains are also necessary for full expression in vivo (14).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A specific domain of the adenovirus EIV promoter is necessary to maintain susceptibility of the integrated promoter to EIA transactivation.

Nishigaki¹,

Hanaka²,

Kingston³

et al. 1988

Mol. Cell. Biol.

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Bovine leukemia virus trans-activator p38tax activates heterologous promoters with a common sequence known as a cAMP-responsive element or the binding site of a cellular transcription factor ATF.

Katoh¹,

Yoshinaka²,

Ikawa³

1989

The EMBO Journal

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The genome of bovine leukemia virus (BLV) encodes a transcriptional trans‐activator p38tax (also referred to as pXBL‐I) which amplifies the virus gene expression driven by its long terminal repeat (LTR). It was proposed that activation of cellular gene expression by p38tax might be involved in the mechanism of B‐cell transformation caused in vivo by BLV infection. Here, we report that the U3 region of BLV LTR contains multiple regulatory elements responsive to p38tax. A core element composing the p38tax‐inducible U3 structure is suggested to be a heptanucleotide motif of 5′TGACGTCA3′, the consensus sequence proposed for a cAMP‐responsive element (CRE) and for the binding sites of a cellular transcription factor (ATF). Adenovirus‐5 E3 and E4, c‐fos and somatostatin regulatory regions containing CRE/ATF‐element exhibited responsiveness to p38tax in a chloramphenicol acetyltransferase transient expression assay. These suggest that in BLV‐infected cells, cellular gene expression might be induced abnormally by the virus trans‐activator through ATF or ATF‐like factors.

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Transcription in the reverse orientation at either terminus of the adenovirus type 5 genome.

et al. 1989

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The right terminus of the adenovirus type 5 (Ad5) genome, which contained the early‐region 4 (E4) promoter, was capable of initiating transcription in the reverse orientation in in vitro and in vivo assays. Multiple cis‐acting elements, required for original‐oriented transcription, were also important for reverse‐oriented transcription except for the original TATA box. We constructed a plasmid in which the E4 promoter region was linked to the chloramphenicol acetyltransferase gene in the original orientation and linked to the lacZ gene in the reverse orientation and tested the ability of a co‐transfected E1A gene to stimulate transcription in both orientations. Activities of transcription in both orientations were stimulated 6‐ to 10‐fold in this assay. More than one cis‐acting element was necessary for the stimulation. The region between −39 and −177 was necessary for the E1A‐mediated trans‐activation of transcription in both orientations, indicating that the region functioned in a bidirectional manner. The activity of transcription in the reverse orientation was similarly detected at the left terminus of the Ad genome and the activity was stimulated several‐fold by E1A gene products.

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Regulation of in vitro and in vivo transcription of early-region IV of adenovirus type 5 by multiple cis-acting elements.

Cited by 35 publications

References 39 publications

A specific domain of the adenovirus EIV promoter is necessary to maintain susceptibility of the integrated promoter to EIA transactivation.

A specific domain of the adenovirus EIV promoter is necessary to maintain susceptibility of the integrated promoter to EIA transactivation.

Bovine leukemia virus trans-activator p38tax activates heterologous promoters with a common sequence known as a cAMP-responsive element or the binding site of a cellular transcription factor ATF.

Transcription in the reverse orientation at either terminus of the adenovirus type 5 genome.

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