Regulation of immunophenotype modulation of monocytes-macrophages from M1 into M2 by prostate cancer cell-culture supernatant via transcription factor STAT3

Solís-Martínez, Raúl; Marentes, Martha Edith Cancino; Hernández‐Flores, Georgina; Ortiz‐Lazareno, Pablo César; Mandujano-Álvarez, G.; Cruz-Gálvez, Claudia Carolina; Sierra-Díaz, Erick; Rodríguez‐Padilla, Cristina; Jave‐Suárez, Luis Felipe; Aguilar‐Lemarroy, Adriana; Bravo-Cuéllar, Alejandro

doi:10.1016/j.imlet.2018.02.009

Cited by 36 publications

(31 citation statements)

References 41 publications

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“…Monocyte-to-macrophage differentiation and polarization, mainly to tumor-associated macrophages (TAM), is a clinical trait of prostate cancer associated with poor prognosis and tumor recurrence [74,75]. Mechanistically, tumors constantly attract myeloid cells from the bloodstream via several secreted factors, such as VEGF, which in turn drives pro-tumorigenic macrophage polarization [76,77]. Plasma treatment decreased VEGF levels in prostate cancer cell supernatants and co-cultures with myeloid cells.…”

Section: Discussionmentioning

confidence: 99%

Gas Plasma-Treated Prostate Cancer Cells Augment Myeloid Cell Activity and Cytotoxicity

et al. 2020

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Despite recent improvements in cancer treatment, with many of them being related to foster antitumor immunity, tumor-related deaths continue to be high. Novel avenues are needed to complement existing therapeutic strategies in oncology. Medical gas plasma technology recently gained attention due to its antitumor activity. Gas plasmas act via the local deposition of a plethora of reactive oxygen species (ROS) that promote the oxidative cancer cell death. The immunological consequences of plasma-mediated tumor cell death are only poorly understood, however. To this end, we exposed two prostate cancer cell lines (LNCaP, PC3) to gas plasma in vitro, and investigated the immunomodulatory effects of the supernatants in as well as of direct co-culturing with two human myeloid cell lines (THP-1, HL-60). After identifying the cytotoxic action of the kINPen plasma jet, the supernatants of plasma-treated prostate cancer cells modulated myeloid cell-related mitochondrial ROS production and their metabolic activity, proliferation, surface marker expression, and cytokine release. Direct co-culture amplified differentiation-like surface marker expression in myeloid cells and promoted their antitumor-toxicity in the gas plasma over the untreated control conditions. The results suggest that gas plasma-derived ROS not only promote prostate cancer cell death but also augment myeloid cell activity and cytotoxicity.

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Section: Discussionmentioning

confidence: 99%

Gas Plasma-Treated Prostate Cancer Cells Augment Myeloid Cell Activity and Cytotoxicity

et al. 2020

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“…Prostate cancer is the most commonly diagnosed non-cutaneous cancer and the second leading cancer-related cause of death for men. In 2016 over 200,000 new cases of prostate cancer were diagnosed in the United States (1). Approximately 307,000 deaths were recorded globally in 2012.…”

Section: Introductionmentioning

confidence: 99%

“…The tumor microenvironment is a chronic site of infl ammation (2). The cancer microenvironment is known to comprise of tumor and stromal cells and involves a complex network of reciprocal interaction between tumor cells and infl ammatory cells (1).…”

Section: Introductionmentioning

confidence: 99%

“…They promote the formation of metastases, angiogenesis (secrete angiogenic factors), tumor growth (producing growth hormones), enhance the migration and invasiveness of tumor cells (6). This effect can be understood via the functional plasticity of macrophages, which can be classifi ed as M1 or M2 (1). Macrophages are recruited to tumors by growth factors and chemokines, which are often produced by the cancer cells and stroma cells in tumor.…”

Section: Introductionmentioning

confidence: 99%

“…Can it be expected that prostatic cancer cells secrete factors, which modulate phenotypic manifestations in macrophages? Modulation of the macrophages immunophenotype from M1 into M2 by the microenvironment is not well understood (1). In this study we aimed on how supernatant from prostate cancer cell line PC-3 effects functional polarization in macrophages.…”

Section: Introductionmentioning

confidence: 99%

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Effect of prostate cancer cell line supernatant on functional polarization in macrophages

Mazalova¹,

Sládek²,

Raudenská³

et al. 2018

BLL

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MiR‐29b regulates Th2 cell differentiation in asthma by targeting inducible B7‐H3 and STAT3

Zhang

et al. 2022

Clinical & Translational All

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Background: MicroRNAs play an important role in T cell responses. However, how microRNAs regulate Th cells in asthma remains poorly defined.Objective: In this study, we investigated the mechanism and pathways of miR-29b regulating Th cells in asthma, in order to find new targets for asthma. Methods:We detected miR-29b, B7-H3 and STAT3 in the peripheral blood of children with asthma, explored the relationship between these molecules and their effects on T cells through in vitro cell culture, and verified it by animal model. Results:MiR-29b levels were decreased in the peripheral blood mononuclear cells from children with asthma. Vitro studies found that the expression of miR-29b in macrophages was decreased and the expression of B7-H3 and STAT3 was increased after house dust mite (HDM) stimulation. After down-regulation of miR-29b in macrophages, the expressions of B7-H3 and STAT3 in macrophages were increased and T cells differentiate into Th2 cells. After the addition of B7-H3 or STAT3 antibodies, the differentiation of naive T cells into Th2 cells was reduced. In OVA induced mice asthmatic model, after the up-regulation of miR-29b in lung, the expression of B7-H3 and STAT3 decreased in the lung tissues of mice, and the expression of Th2 cells and type II cytokine decreased simultaneously. The pathological changes of lung tissues were also alleviated. Conclusion:The expression of miR-29b is decreased in asthmatic children. MiR-29b can inhibit Th2 cell differentiation by inhibiting B7-H3 and STAT3 pathways at the same time, and reduce asthmatic immune inflammation.

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Regulation of immunophenotype modulation of monocytes-macrophages from M1 into M2 by prostate cancer cell-culture supernatant via transcription factor STAT3

Cited by 36 publications

References 41 publications

Gas Plasma-Treated Prostate Cancer Cells Augment Myeloid Cell Activity and Cytotoxicity

Gas Plasma-Treated Prostate Cancer Cells Augment Myeloid Cell Activity and Cytotoxicity

Effect of prostate cancer cell line supernatant on functional polarization in macrophages

MiR‐29b regulates Th2 cell differentiation in asthma by targeting inducible B7‐H3 and STAT3

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