Regulation of immune responses to Mycobacterium tuberculosis secretory antigens by dendritic cells

Latchumanan, Vinoth K.; Balkhi, Mumtaz Yaseen; Sinha, Aprajita; Singh, Balwan; Sharma, Pawan; Natarajan, Krishnamurthy

doi:10.1016/j.tube.2005.08.007

Cited by 15 publications

(14 citation statements)

References 50 publications

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“…We observed consistently reduced modulation of immature MoDC by S7 and Rv infection. Though not significant, there was slight upregulation of MHC-II molecule expression on all infected MoDC, imparting the fact that there was shift from the immature stage with all the strains [26]. It was found that Rv and S7 infected MoDC had greatly reduced CD83 levels and the expression of B7.1 (CD 80) and B7.2 (CD86) levels were also not enhanced efficiently after the infection with these strains, depicting that the expression of these molecules were differentially regulated on the cells depending upon the type of M.tb strain [27].…”

Section: Discussionmentioning

confidence: 77%

Infection with prevalent clinical strains of Mycobacterium tuberculosis leads to differential maturation of monocyte derived dendritic cells

Rajashree

Das

2008

Immunology Letters

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Section: Discussionmentioning

confidence: 77%

Infection with prevalent clinical strains of Mycobacterium tuberculosis leads to differential maturation of monocyte derived dendritic cells

Rajashree

Das

2008

Immunology Letters

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“…We have shown that MTSA and many other secretory Ags activate DCs by inducing their differentiation and maturation (reviewed in Refs. [13][14][15]. However, a challenge of these DCs with either M. tuberculosis cell extract (CE) (16) or live M. bovis BCG (our unpublished data) down-regulates primary and recall Th1 responses.…”

Section: Impaired Generation Of Reactive Oxygen Species During Differmentioning

confidence: 79%

“…DCs were differentiated with either GM-CSF or MTSA as described before (12)(13)(14). Briefly, bone marrow from the tibias and femurs of BALB/c mice were flushed out and lymphocytes and I-A ϩ cells were depleted following MACS.…”

Section: Enrichment Of DC Precursors From Bone Marrow and Generation mentioning

confidence: 99%

Impaired Generation of Reactive Oxygen Species during Differentiation of Dendritic Cells (DCs) byMycobacterium tuberculosisSecretory Antigen (MTSA) and Subsequent Activation of MTSA-DCs by Mycobacteria Results in Increased Intracellular Survival

Sinha

Singh

Satchidanandam

et al. 2006

The Journal of Immunology

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We investigated the role of reactive oxygen species (ROS) in dendritic cell (DC) differentiation by 10-kDa Mycobacterium tuberculosis secretory Ag (MTSA) and survival of mycobacteria therein. Compared with GM-CSF, MTSA induced lower ROS production during DC differentiation from precursors. This result correlated with higher superoxide dismutase 1 expression in MTSA stimulated precursors as compared with GM-CSF stimulation. Furthermore, a negative regulation of protein kinase C (PKC) activation by ROS was observed during DC differentiation. ROS inhibited the rapid and increased phosphorylation of PKCα observed during DC differentiation by MTSA. In contrast, ROS inhibition increased the weak and delayed PKCα phosphorylation by GM-CSF. Similar to DC differentiation, upon activation with either M. tuberculosis cell extract (CE) or live Mycobacterium bovis bacillus Calmette-Guérin (BCG), DCs differentiated with MTSA (MTSA-DCs) generated lower ROS levels when compared with DCs differentiated with GM-CSF (GM-CSF-DCs). Likewise, a negative regulation of PKCα phosphorylation by ROS was once again observed in DCs activated with either M. tuberculosis CE or live M. bovis BCG. However, a reciprocal positive regulation between ROS and calcium was observed. Compared with MTSA-DCs, stimulation of GM-CSF-DCs with M. tuberculosis CE induced a 2-fold higher ROS-dependent calcium influx. However, pretreatment of MTSA-DCs with H2O2 increased calcium mobilization. Finally, lower ROS levels in MTSA-DCs correlated with increased intracellular survival of M. bovis BCG when compared with survival in GM-CSF-DCs. Although inhibiting ROS in GM-CSF-DCs increased M. bovis BCG survival, H2O2 treatment of MTSA-DCs decreased survival of M. bovis BCG. Overall our results suggest that DCs differentiated with Ags such as MTSA may provide a niche for survival and/or growth of mycobacteria following sequestration of ROS.

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“…TGF-β is secreted by a large number of cell types including monocytes, macrophages, DCs, and CD4+ regulatory T cells (Aung et al, 2005; Latchumanan et al, 2005). TGF-β is shown to synergize with IL-10 to promote immune tolerance and limit pathological inflammation (Oswald et al, 1992; Zeller et al, 1999; Clegg and Hughes, 2002; Chen et al, 2003).…”

Section: Immunomodulatory Cytokinesmentioning

confidence: 99%

Mycobacterium Tuberculosis Infection and Inflammation: what is Beneficial for the Host and for the Bacterium?

Sasindran¹,

Torrelles²

2011

Front. Microbio.

212

186

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Tuberculosis is still a major health problem in the world. Initial interactions between Mycobacterium tuberculosis and the host mark the pathway of infection and the subsequent host inflammatory response. This inflammatory response is tightly regulated by both the host and the bacterium during different stages of infection. As infection progresses, the initial intense pro-inflammatory response observed is regulated by suppressive mediators balancing inflammation. In this environment, M. tuberculosis battles to survive interfering with the host inflammatory response. In this review we discuss the major effector molecules involved in inflammation in relation to the different stages of M. tuberculosis infection.

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Regulation of immune responses to Mycobacterium tuberculosis secretory antigens by dendritic cells

Cited by 15 publications

References 50 publications

Infection with prevalent clinical strains of Mycobacterium tuberculosis leads to differential maturation of monocyte derived dendritic cells

Infection with prevalent clinical strains of Mycobacterium tuberculosis leads to differential maturation of monocyte derived dendritic cells

Impaired Generation of Reactive Oxygen Species during Differentiation of Dendritic Cells (DCs) byMycobacterium tuberculosisSecretory Antigen (MTSA) and Subsequent Activation of MTSA-DCs by Mycobacteria Results in Increased Intracellular Survival

Mycobacterium Tuberculosis Infection and Inflammation: what is Beneficial for the Host and for the Bacterium?

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