Regulation of
            <i>PPARGC1A</i>
            gene expression in trained and untrained human skeletal muscle

Popov, D. V.; Лысенко, Е. А.; Makhnovskii, Pavel A.; Kurochkina, Nadia S; Виноградова, О. Л.

doi:10.14814/phy2.13543

Cited by 15 publications

(19 citation statements)

References 51 publications

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“…RNA extraction, library construction, and sequencing. RNA was extracted from the frozen samples (~20 mg) using an RNeasy mini kit (Qiagen, Hilden, Germany) with DNase treatment (Fermentas, Vilnius, Lithuania), as described elsewhere (32). The RNA concentration was measured in a fluorimeter (Qubit 3.0; Thermo Scientific, Vilnius, Lithuania), and RNA integrity was checked by capillary electrophoresis using a Bioanalyzer 2100 (Agilent, Santa Clara, CA); all samples were at least 7 in RIN.…”

Section: Methodsmentioning

confidence: 99%

Contractile activity-specific transcriptome response to acute endurance exercise and training in human skeletal muscle

Popov

Makhnovskii

Shagimardanova

et al. 2019

American Journal of Physiology-Endocrinology and Metabolism

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Reduction in daily activity leads to dramatic metabolic disorders, while regular aerobic exercise training is effective for preventing this problem. The purpose of this study was to identify genes that are directly related to contractile activity in human skeletal muscle, regardless of the level of fitness. Transcriptome changes after the one-legged knee extension exercise in exercised and contralateral nonexercised vastus lateralis muscle of seven men were evaluated by RNA-seq. Transcriptome change at baseline after 2 mo of aerobic training (5/wk, 1 h/day) was evaluated as well. Postexercise changes in the transcriptome of exercised muscle were associated with different factors, including circadian oscillations. To reveal transcriptome response specific for endurance-like contractile activity, differentially expressed genes between exercised and nonexercised muscle were evaluated at 1 and 4 h after the one-legged exercise. The contractile activity-specific transcriptome responses were associated only with an increase in gene expression and were regulated mainly by CREB/ATF/AP1-, MYC/MAX-, and E2F-related transcription factors. Endurance training-induced changes (an increase or decrease) in the transcriptome at baseline were more pronounced than transcriptome responses specific for acute contractile activity. Changes after training were associated with widely different biological processes than those after acute exercise and were regulated by different transcription factors (IRF- and STAT-related factors). In conclusion, adaptation to regular exercise is associated not only with a transient (over several hours) increase in expression of many contractile activity-specific genes, but also with a pronounced change (an increase or decrease) in expression of a large number of genes under baseline conditions.

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Section: Methodsmentioning

confidence: 99%

Contractile activity-specific transcriptome response to acute endurance exercise and training in human skeletal muscle

Popov

Makhnovskii

Shagimardanova

et al. 2019

American Journal of Physiology-Endocrinology and Metabolism

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“…The anaerobic threshold (AT) of the m. vastus lateralis (measured by electromyography and changes in deoxyhemoglobin content) were evaluated as described previously (Popov et al. ). The initial load, load increment, and knee extension rate were 0 W, 2.5 W/min, and 60 cycles/min, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…All participants provided written informed consent to participate. The study design has been described elsewhere (Popov et al 2017). Briefly, 10 untrained males (median age, 22 years [interquartile range, 21-26 years]; weight, 74 kg [72-79 kg]; and body mass index, 23 kg/m 2 [22-25 kg/m 2 ]) performed a onelegged continuous knee extension exercise at the same relative intensity before and after 2 months of aerobic training.…”

Section: Methodsmentioning

confidence: 99%

“…Each participant performed an incremental one-legged ramp test on a modified electromagnetic ergometer (Ergometric 900S, Ergoline) until exhaustion. The anaerobic threshold (AT) of the m. vastus lateralis (measured by electromyography and changes in deoxyhemoglobin content) were evaluated as described previously (Popov et al 2017). The initial load, load increment, and knee extension rate were 0 W, 2.5 W/min, and 60 cycles/min, respectively.…”

Section: One-legged Knee Extension Ramp Testmentioning

confidence: 99%

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Effect of aerobic training on baseline expression of signaling and respiratory proteins in human skeletal muscle

et al. 2018

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Most studies examining the molecular mechanisms underlying adaptation of human skeletal muscles to aerobic exercise focused on the response to acute exercise. Here, we examined the effect of a 2‐month aerobic training program on baseline parameters in human muscle. Ten untrained males performed a one‐legged knee extension exercise for 1 h with the same relative intensity before and after a 2‐month aerobic training program. Biopsy samples were taken from vastus lateralis muscle at rest before and after the 2 month training program (baseline samples). Additionally, biopsy samples were taken from the exercised leg 1 and 4 h after the one‐legged continuous knee extension exercise. Aerobic training decreases baseline phosphorylation of FOXO1Ser256, increases that of CaMKIIT hr286, CREB1Ser133, increases baseline expression of mitochondrial proteins in respiratory complexes I–V, and some regulators of mitochondrial biogenesis (TFAM, NR4A3, and CRTC2). An increase in the baseline content of these proteins was not associated with a change in baseline expression of their genes. The increase in the baseline content of regulators of mitochondrial biogenesis (TFAM and NR4A3) was associated with a transient increase in transcription after acute exercise. Contrariwise, the increase in the baseline content of respiratory proteins does not seem to be regulated at the transcriptional level; rather, it is associated with other mechanisms. Adaptation of human skeletal muscle to regular aerobic exercise is associated not only with transient molecular responses to exercise, but also with changes in baseline phosphorylation and expression of regulatory proteins.

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“…It is also important to note that our results are likely to be specific for the participants recruited and the exercise employed. It has previously been reported that both training and fitness level can affect exercise-induced gene expression 44,45 . Thus, the time courses we have reported cannot be assumed for other populations.…”

Section: Compared 19mentioning

confidence: 99%

Interpretation of exercise-induced changes in human skeletal muscle mRNA expression depends on the timing of the post-exercise biopsies

Kuang

McGinley

Lee

et al. 2020

Preprint

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AimExercise elicits a range of adaptive responses in skeletal muscle that include changes in mRNA expression. To better understand the health benefits of exercise training, it is essential to investigate the underlying molecular mechanisms of skeletal muscle adaptations to exercise. However, most studies have assessed the molecular events at a few convenient time points within a short time frame post exercise, and the variations of gene expression kinetics have not been addressed systematically.MethodMuscle biopsies were collected from nine participants at baseline and six time points (0, 3, 9, 24, 48, and 72 h) following a session of high-intensity interval exercise. We assessed the mRNA content of 23 gene isoforms from the muscle samples.ResultThe temporal patterns of target gene expression were highly variable and the mRNA contents detected were largely dependent on the muscle sample timing. The maximal levels of mRNA content of all tested target genes were observed between 3 to 48 h post exercise.ConclusionOur findings highlight a critical gap in knowledge regarding the molecular response to exercise, where the use of a few time points within a short period after exercise has led to an incomplete understanding of the molecular responses to exercise. The timing of muscle sampling for individual studies needs to be carefully chosen based on existing literature and preliminary analysis of the molecular targets of interest. We propose that a comprehensive time-course analysis on the exercise-induced transcriptional response in humans will significantly benefit the field of exercise molecular biology.

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Regulation of PPARGC1A gene expression in trained and untrained human skeletal muscle

Cited by 15 publications

References 51 publications

Contractile activity-specific transcriptome response to acute endurance exercise and training in human skeletal muscle

Contractile activity-specific transcriptome response to acute endurance exercise and training in human skeletal muscle

Effect of aerobic training on baseline expression of signaling and respiratory proteins in human skeletal muscle

Interpretation of exercise-induced changes in human skeletal muscle mRNA expression depends on the timing of the post-exercise biopsies

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