Regulation of<i>FUSCA3</i>Expression During Seed Development in Arabidopsis

Roscoe, Thomas; Vaissayre, Virginie; Paszkiewicz, Gaël; Clavijo, Fernando; Kelemen, Zsolt; Michaud, Caroline; Lepiniec, Loïc; Dubreucq, Bertrand; Zhou, Dao‐Xiu; Devic, Martine

doi:10.1093/pcp/pcy224

Cited by 26 publications

(12 citation statements)

References 76 publications

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“…Furthermore, ectopic FUS3 in bpc1/2, bpc1 pFUS3:FUS3-GFP or pML1:FUS3-GFP leads to similar phenotypes, including reduced internode elongation and defective flowers (arrested flower bud development, flowers with a protruding carpel and shorter floral organs), suggesting FUS3 inhibits the elongation of the stem and floral organs during flowering. Recently, deletion of a small region in the FUS3 promoter near the BPC binding sites and corresponding to the PRC2 recruitment region, lead to ectopic FUS3 expression in vegetative tissues (Roscoe et al, 2019). Thus, we propose that class I BPCs recruit VRN/EMF-PRC2 to repress FUS3 postembryonically, more specifically in germinating seeds, in vegetative and reproductive organs (Figure 10).…”

Section: Class I Bpcsmentioning

confidence: 83%

Spatiotemporal Restriction of FUSCA3 Expression by Class I BPCs Promotes Ovule Development and Coordinates Embryo and Endosperm Growth

Mohamed

Dowhanik

et al. 2020

Plant Cell

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Spatiotemporal regulation of gene expression plays an important role in developmental timing in plants and animals. FUSCA3 regulates the transition between different phases of development by acting as a link between different hormonal pathways in Arabidopsis.However, the mechanisms governing its spatiotemporal expression patterns are poorly understood. Here, we show that FUS3 is expressed in the chalaza and funiculus of the mature ovule and seed, but is repressed in the embryo sac, integuments and endosperm. FUS3 repression requires class I BASIC PENTACYSTEINE (BPC) proteins, which directly bind to the FUS3 locus and restrict its expression pattern. During vegetative and reproductive development, derepression of FUS3 in bpc1/2 or pML1:FUS3 misexpression lines results in dwarf plants carrying defective flowers and aborted ovules. Post-fertilization, ectopic FUS3 expression in the endosperm increases endosperm nuclei proliferation and seed size and delays or arrests embryo development. These phenotypes are rescued in bpc1/2 fus3-3. Lastly, class I BPCs interact with FIS-PRC2 (FERTILIZATION-INDEPENDENT SEED-Polycomb Repressive Complex 2), which represses FUS3 in the endosperm. We propose that BPC1/2 promotes the transition from reproductive to seed development by repressing FUS3 in ovule integuments. After fertilization, BPC1/2 and FIS-PRC2 repress FUS3 in the endosperm to coordinate endosperm and embryo growth.

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Section: Class I Bpcsmentioning

confidence: 83%

Spatiotemporal Restriction of FUSCA3 Expression by Class I BPCs Promotes Ovule Development and Coordinates Embryo and Endosperm Growth

Mohamed

Dowhanik

et al. 2020

Plant Cell

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“…This is the first stage at which all the gene products described as important for the expression of maturation genes are present at the same time in the embryo proper. While LEC1 , L1L , ABI3 , bZIP10 and 53 , and NF‐YC2 are expressed from early in development (Parcy et al ., ; Lotan et al ., ; Kwong et al ., ; Belmonte et al ., ), LEC2 does not appear in the embryo proper at detectable levels until the early‐ to mid‐globular stages (Stone et al ., ; Kroj et al ., ) (but possibly earlier, see (Hofmann et al ., )), and FUS3 not until the late globular to early heart stages (Kroj et al ., ; Tsuchiya et al ., ) (but see (Roscoe et al ., ) for a suggested earlier onset). The key, then, is likely to be in the regulation of the LAFL genes, of which not much is known.…”

Section: Discussionmentioning

confidence: 94%

The onset of embryo maturation in Arabidopsis is determined by its developmental stage and does not depend on endosperm cellularization

2019

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Seeds are dormant and desiccated structures, filled with storage products to be used after germination. These properties are determined by the maturation program, which starts, in Arabidopsis thaliana, midembryogenesis, at about the same time and developmental stage in all the seeds in a fruit. The two factors, chronological and developmental time, are closely entangled during seed development, so their relative contribution to the transition to maturation is not well understood. It is also unclear whether that transition is determined autonomously by each seed or whether it depends on signals from the fruit. The onset of maturation follows the cellularization of the endosperm, and it has been proposed that there exists a causal relationship between both processes. We explored all these issues by analyzing markers for maturation in Arabidopsis mutant seeds that develop at a slower pace, or where endosperm cellularization happens too early, too late, or not at all. Our data show that the developmental stage of the embryo is the key determinant of the initiation of maturation, and that each seed makes that transition autonomously. We also found that, in contrast with previous models, endosperm cellularization is not required for the onset of maturation, suggesting that this transition is independent of the hexose/sucrose ratio in the seed. Our observations indicate that the mechanisms that control endosperm cellularization, embryo growth, and embryo maturation act independently of each other.

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“…Previous work has shown that transient activation or ectopic expression of one or several transcription factors can trigger the transition from NEC to EC or increase the frequency of EC formation. These include WOX5 [73], WUSHEL [74], WRKY2 [75], GRD/RKD4 [76], BBM [40], LEC1 [54], FUS3 [77], ABI3 [78], and AGL15 [79]. For example, the AP2/ERF TF BBM in plants induced plentiful cell regeneration and was used to produce a large number of somatic embryos that could developed to seedlings [80].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)

et al. 2020

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Background Genotype independent transformation and whole plant regeneration through somatic embryogenesis relies heavily on the intrinsic ability of a genotype to regenerate. The critical genetic architecture of non-embryogenic callus (NEC) cells and embryogenic callus (EC) cells in a highly regenerable cotton genotype is unknown. Results In this study, gene expression profiles of a highly regenerable Gossypium hirsutum L. cultivar, Jin668, were analyzed at two critical developmental stages during somatic embryogenesis, non-embryogenic callus (NEC) cells and embryogenic callus (EC) cells. The rate of EC formation in Jin668 is 96%. Differential gene expression analysis revealed a total of 5333 differentially expressed genes (DEG) with 2534 genes upregulated and 2799 genes downregulated in EC. A total of 144 genes were unique to NEC cells and 174 genes were unique to EC. Clustering and enrichment analysis identified genes upregulated in EC that function as transcription factors/DNA binding, phytohormone response, oxidative reduction, and regulators of transcription; while genes categorized in methylation pathways were downregulated. Four key transcription factors were identified based on their sharp upregulation in EC tissue; LEAFY COTYLEDON 1 (LEC1), BABY BOOM (BBM), FUSCA (FUS3) and AGAMOUS-LIKE15 with distinguishable subgenome expression bias. Conclusions This comparative analysis of NEC and EC transcriptomes gives new insights into the genes involved in somatic embryogenesis in cotton.

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Regulation ofFUSCA3Expression During Seed Development in Arabidopsis

Cited by 26 publications

References 76 publications

Spatiotemporal Restriction of FUSCA3 Expression by Class I BPCs Promotes Ovule Development and Coordinates Embryo and Endosperm Growth

Spatiotemporal Restriction of FUSCA3 Expression by Class I BPCs Promotes Ovule Development and Coordinates Embryo and Endosperm Growth

The onset of embryo maturation in Arabidopsis is determined by its developmental stage and does not depend on endosperm cellularization

Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)

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