Regulation of hyaluronate metabolism by progesterone in cultured fibroblasts from the human uterine cervix

Nakamura, Toshiya; Takagaki, Keiichi; Funahashi, Masaru; Saito, Yoshiharu; Endo, Masahiko

doi:10.1016/s0014-5793(96)01538-4

Cited by 30 publications

(18 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…P inhibits matrix metalloproteinase (MMP) activities (Imada et al, 1997;Sato et al, 1991) and stimulates tissue inhibitor of metalloproteinase (TIMP)-2 in rabbit uterine cervical fibroblasts ; thus, it is considered to also exert a potent inhibitory action on cervical ripening. Our previous results have shown that P suppresses hyaluronate synthesis in human uterine cervical fibroblasts (Tanaka et al, 1994(Tanaka et al, , 1997. One of the principle components of the uterine cervix is PGs.…”

Section: Discussionmentioning

confidence: 93%

“…Although further studies are needed to evaluate the effectiveness of each method, it has been reported that P is promising because the hormone can inhibit prostaglandin production and reduce myometrial contractility, thus maintaining uterine quiescence. We have shown that P suppresses hyaluronate synthesis in human uterine cervical fibroblasts (Tanaka et al, 1994(Tanaka et al, , 1997. Hyaluronate is an extracellular matrix and has been shown to increase prior to parturition in the human uterine cervix, and is thus considered to play an important role in cervical ripening (Osmers et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anti-inflammatory effect of proteoglycan and progesterone on human uterine cervical fibroblasts

et al. 2012

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory effect of proteoglycan and progesterone on human uterine cervical fibroblasts

et al. 2012

Self Cite

View full text Add to dashboard Cite

“…Gel-filtration HPLC of hyaluronic acid was carried out as described previously (5). The reaction product of cell-free synthesis was applied to a Shodex OHpak KB-805 column (8.0 mm inner diameter x 30 cm; Showa Denko, Tokyo, Japan).…”

Section: Introductionmentioning

confidence: 99%

Effect of 4‐methylumbelliferone on cell‐free synthesis of hyaluronic acid

et al. 1997

Self Cite

View full text Add to dashboard Cite

Summary: Human skin fibroblasts were cultured in the presence of 0.5 mM 4-methylumbelliferone for 12 h, and cell-free synthesis of hyaluronic acid was performed using membrane-rich fraction from the ceils. The preincubation of the cells with 4-mcthylumbelliferone reduced hyaluronic acid synthesis to 15% of that of non-preincubated cells, although its chain length was not changed. On the other hand, without preincubation of the cells with 4-methylumbelliferone, hyaluronic acid synthesis was not changed even when 4-methylumbelliferone was added directly to the reaction mixture. These results suggest that 4-methylumbelliferone represses the expression of hyaluronic acid synthase on the cell surface.

show abstract

“…P 4 is essential in pregnancy, and high levels of this hormone are maintained during gestation (Hardy et al 2006). Cervical remodelling during pregnancy depends on P 4 because it can modulate the expression of some components of the extracellular matrix (ECM), thus inhibiting hyaluronate expression (Tanaka et al 1994(Tanaka et al , 1997 and increasing collagenase, elastase and metalloproteinase levels (Andersson et al 2008). Because the cervical tissue mechanical properties are related to the ECM (Myers et al 2008) and because the ECM is the major component of cervical tissue (Leppert 1995), cervical softening is associated with decreased collagen organisation, which is characterised by increased collagen solubility and decreased collagen concentration.…”

Section: Discussionmentioning

confidence: 99%

KCNH1 potassium channels are expressed in cervical cytologies from pregnant patients and are regulated by progesterone

et al. 2013

View full text Add to dashboard Cite

Potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1) potassium channels are potential tumour markers and cancer therapeutic targets and are up-regulated by oestrogens and human papilloma virus (HPV) oncogenes. However, the role of KCNH1 in normal tissues is poorly understood, and its expression in pregnancy is unknown. We wondered whether KCNH1 channels are expressed in cervical cells from pregnant patients and whether progesterone (P 4 ) regulates KCNH1. The association with HPV was also investigated. KCNH1 protein expression was studied by immunocytochemistry in liquid-based cervical cytologies; 93 samples were obtained from pregnant patients at different trimesters, and 15 samples were obtained from non-pregnant women (controls). The presence of HPV was studied by PCR with direct sequencing and nested multiplex PCR. HeLa cervical cancer cells were transfected with human progesterone receptor-B (PR-B) and treated with P 4 . KCNH1 mRNA expression in these cultures was studied by real-time PCR. KCNH1 protein was detected in 100% of the pregnancy samples and in 26% of the controls. We found 18 pregnant patients infected with HPV and detected 14 types of HPV. There was no association between the percentage of cells expressing KCNH1 and either the presence or type of HPV. P 4 induced KCNH1 mRNA and protein expression in cells transfected with human PR-B. No regulation of KCNH1 by P 4 was observed in non-transfected cells. We show for the first time the expression of an ion channel during human pregnancy at different trimesters and KCNH1 regulation by P 4 in human cells. These data raise a new research field for KCNH1 channels in human tissues.

show abstract

Regulation of hyaluronate metabolism by progesterone in cultured fibroblasts from the human uterine cervix

Abstract: These results suggest that in cultured fibroblasts from the human uterine cervix progesterone converts hyaluronate metabolism from the synthesis phase to the degradation phase.

Cited by 30 publications

References 12 publications

Anti-inflammatory effect of proteoglycan and progesterone on human uterine cervical fibroblasts

Anti-inflammatory effect of proteoglycan and progesterone on human uterine cervical fibroblasts

Effect of 4‐methylumbelliferone on cell‐free synthesis of hyaluronic acid

KCNH1 potassium channels are expressed in cervical cytologies from pregnant patients and are regulated by progesterone

Contact Info

Product

Resources

About