Regulation of human head and neck squamous cell carcinoma growth in tissue culture by opioid growth factor.

McLaughlin, Patricia J.; Levin, Roger J.; Zagon, Ian S.

doi:10.3892/ijo.14.5.991

Cited by 39 publications

(81 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With respect to human squamous cell carcinoma of the head and neck (SCCHN), the OGF-OGFr axis has been shown to be present (4,7,(16)(17)(18)(19) and functional (4,7,16,20,21). Clinical studies assessing the level of OGFr in surgical specimens of SCCHN (19) indicated that OGFr binding sites and protein levels were 9-fold and 5-fold, respectively, less in tissues of patients with SCCHN than in normal epithelium from patients undergoing uvulapalatoplasty or tonsillectomy.…”

Section: Introductionmentioning

confidence: 99%

“…The mechanism of action appears to be that OGF upregulates the cyclindependent kinase inhibitor pathway in order to delay the G 0 /G 1 phase of the cell cycle (14). OGF and OGFr have been demonstrated by immunohistochemistry, radioimmunoassay, and/or receptor binding to be present in a variety of human and animal tumors and cancer cell lines (1)(2)(3)(4)(5)8,(15)(16)(17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

“…The opioid growth factor (OGF) ([Met 5 ]-enkephalin) and its receptor, OGFr, have been well documented in the regulation of growth of a variety of human cancer cells in culture (1)(2)(3)(4)(5) and tumors in nude mice (6)(7)(8)(9). OGF serves as an autocrineproduced, endogenous pentapeptide that inhibits cell proliferation in a receptor-mediated manner (1)(2)(3)(4)(5)10).…”

Section: Introductionmentioning

confidence: 99%

“…OGF serves as an autocrineproduced, endogenous pentapeptide that inhibits cell proliferation in a receptor-mediated manner (1)(2)(3)(4)(5)10). OGF activity is not related to apoptosis/necrosis (11) or differentiation (12), and acts in an anchorage-independent manner (13).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Progression of squamous cell carcinoma of the head and neck is associated with down-regulation of the opioid growth factor receptor

McLaughlin

Zagon

2006

Int J Oncol

View full text Add to dashboard Cite

Abstract. Opioid growth factor (OGF) is a native opioid peptide ([Met 5 ]-enkephalin) that interacts with the OGF receptor (OGFr). OGF serves as a tonically active negative growth factor in neoplasia, and the OGF-OGFr axis contributes to the maintenance of an equilibrium in cell proliferation by targeting the cyclin-dependent inhibitory kinase pathway. To inquire whether the expression of OGFr is related to tumor progression, cell lines of human squamous cell carcinoma of the head and neck (SCCHN) were transplanted into nude mice, and small, medium, and large tumors were assessed for OGFr by receptor binding assays and quantitative immunohistochemistry, and for gene expression of OGFr mRNA. Large tumors had a reduction of 3-to 7-fold in OGFr binding sites relative to small tumors, and medium size tumors showed a progressive diminishment in OGF receptors that ranged between that of small and large neoplasias. Tumors with xenografts of three different cell lines of SCCHN, representing poorly-and well-differentiated cancers, exhibited similar results. Quantitative densitometric immunohistochemistry revealed data comparable to receptor binding assays. Receptor affinity and the gene expression of OGFr mRNA were unchanged in tumors of different sizes. These data demonstrate that OGFr is reduced in SCCHN with tumor progression and that translation/ posttranslation of OGFr protein, but not transcriptional levels of the OGFr gene, is (are) involved. The attenuated levels of OGFr binding capacity may serve as a marker of SCCHN. These subnormal levels of OGFr may diminish the efficacy of the OGF-OGFr axis in maintaining cell proliferative activity, and contribute to more active cell replication. Gene therapy to reinstate more OGFr, and thus enhance OGFr function, could serve as a useful treatment for inhibiting tumor progression.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Progression of squamous cell carcinoma of the head and neck is associated with down-regulation of the opioid growth factor receptor

McLaughlin

Zagon

2006

Int J Oncol

View full text Add to dashboard Cite

show abstract

“…OGF interacts with the OGF receptor (OGFr) to delay the G 1 /S interface of the cell cycle by modulating cyclin-dependent kinase inhibitory pathways (4 -6). Attenuation of the OGF-OGFr axis in cancer cells through: 1) disruption of OGF-OGFr interfacing by continuous exposure to opioid antagonists [e.g., naltrexone (NTX)] (2, 15, 34), 2) neutralization of OGF by antibodies to the peptide (15), or 3) a decrease in OGFr by antisense cDNA or siRNA for OGFr (26,37), stimulates cell proliferation. An increase in OGF-OGFr activity in cancer cells by 1) addition of exogenous OGF (2,15,34); 2) treatment with imidazoquinoline compounds, such as imiquimod and resiquimod (26); or 3) transfection of sense cDNA for OGFr (18,35), depresses cell proliferation.…”

mentioning

confidence: 99%

The opioid growth factor-opioid growth factor receptor axis regulates cell proliferation of human hepatocellular cancer

Avella¹,

Kimchi²,

Donahue

et al. 2010

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

Nucleocytoplasmic distribution of opioid growth factor and its receptor in tongue epithelium

2004

View full text Add to dashboard Cite

The subcellular distributions of the opioid growth factor (OGF), [Met 5 ]-enkephalin, and opioid growth factor receptor (OGFr) in the epithelium of the rat tongue were determined in order to reveal structure-function relationships. Laser scanning confocal microscopic analysis showed that both OGF and OGFr were colocalized in the paranuclear cytoplasm and in the nuclei of keratinocytes in the stratum basale. Using immunoelectron microscopy and postembedding techniques, double labeling experiments disclosed that complexes of OGF-OGFr were colocalized on the outer nuclear envelope, in the paranuclear cytoplasm, perpendicular to the nuclear envelope in a putative nuclear pore complex, and in the nucleus adjacent to heterochromatin. Anti-OGF IgG alone was detected in the cytoplasm, and anti-OGFr IgG alone was associated with the outer nuclear envelope. Study of chronic treatment with the opioid antagonist, naltrexone (NTX), which blocks opioid-receptor binding, revealed the presence of OGFr immunoreactivity alone in the cytoplasm and the nucleus; some OGF-OGFr complexes were also observed. Colocalization of OGFr and karyopherin (importin) ␤ was recorded in the cytoplasm and nucleus. These results in tongue epithelium are the first to suggest that OGFr resides on the outer nuclear envelope, where OGF interacts with OGFr; that the OGF-OGFr complex translocates between cytoplasm and nucleus at the nuclear pore; and that the nuclear localization signal of OGFr interacts with karyopherin ␤ for nuclear transport. These novel data also indicate that signal transduction for cell proliferation appears to involve an OGF-OGFr complex that interfaces with chromatin in the nucleus. Moreover, the unique finding that OGFr was found in the cytoplasm and nucleus in NTXtreated specimens may suggest that NTX-OGFr complexes have the same pathway as OGF-OGFr.

show abstract

Regulation of human head and neck squamous cell carcinoma growth in tissue culture by opioid growth factor.

Cited by 39 publications

References 0 publications

Progression of squamous cell carcinoma of the head and neck is associated with down-regulation of the opioid growth factor receptor

Progression of squamous cell carcinoma of the head and neck is associated with down-regulation of the opioid growth factor receptor

The opioid growth factor-opioid growth factor receptor axis regulates cell proliferation of human hepatocellular cancer

Nucleocytoplasmic distribution of opioid growth factor and its receptor in tongue epithelium

Contact Info

Product

Resources

About