Regulation of hormonal secretion in primary cell cultures of human somatotrophinomas

Bulatov, A. A.; Komolov, I. S.; Smirnova, N B; Trunin, Yu. K.; Serpukhovitin, S. Yu.; Dzeranova, Larisa; Марова, Е И; Дедов, И И

doi:10.1007/bf00840946

Cited by 4 publications

(3 citation statements)

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“…SS and SDS inhibited secretion of both STH and PRL in a dose-dependent manner in the cells of mixed PRL-and STH-secreting pituitary adenoma (patient B). We also earlier demonstrated a high sensitivity of lactotrophs to SS in STH-secreting adenomas comparable to the reaction of somatotrophs [2].…”

Section: Resultsmentioning

confidence: 89%

“…Earlier studies [2] on lactotroph-containing somatotropinomas showed that TRH stimulated PRL secretion in cell cultures in a dose-dependdnt manner one hour after addition to the m e d i u m , while the p r e s e n t study on lactotrophic adenomas showed that TRH only once brought about a reliable, though small, elevation of the PRL level, and only at a maximal concentration of 10 ng/ml in the incubation m e d i u m ( Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…Earlier studies [2,5] on monolayer cultures of pituitary adenoma cells in patients with acromegaly described morphological and functional characteristics of somatotropic adenoma cells, including characteristic features of secretion of somatotropic hormone (STH), as well as PRL, which is synthesized in abundance by adenoma cells. vestigations on lactotrophic adenoma cells, we were confronted with the need to search for other ways of their long-term preservation in vitro due to insufficient adhesive ability of these cells and their low survival rate under conditions similar to those used earlier during culturing with somatotropin.…”

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Morphological and functional characteristics of human pituitary lactotrophic adenomas in cell cultures

et al. 1994

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Some features of the morphological cellular structure of prolactin secreting human pituitary adenomas and their secretion of prolactin and somatotropic hormone in primary suspension cultures were investigated. A possible in vitro proliferation of lactotrophs was established. The inhibitory effect of somatostatin and its synthetic analog sandostatin, on prolactin secretion in prolactinomas was found to be less than in somatotropic hormone-secreting pituitary tumors.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Morphological and functional characteristics of human pituitary lactotrophic adenomas in cell cultures

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Secretory reactions of cultured hypophyseal growth hormone-producing adenoma cells to somatoliberin and somatostatin

et al. 1997

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Somatoliberin stimulates secretion of growth hormone and has no effect on secretion of prolactin in primary cultures of hypophyseal adenoma cells obtained from acromegalic patients. A short-term contact of the cells with somatostatin inhibits secretion of growth hormone, while a long-term contact with this hormone inhibits prolactin production. Somatoliberin abolishes the inhibitory effect of somatostatin on the growth hormone secretion and at high concentrations stimulates it.Key Words: hypophyseal adenoma; growth hormone; prolactin; somatoliberin; somatostatin; cell cultures Generally, hypophyseal tumors producing growth hormone (GH) are benign neoplasms. They consist of adenomatous glandular cells secreting considerable amounts of GH in the circulation, which results in acromegaly, homeostatic disturbances, and serious chronic disease.Secretion of GH is often accompanied by secretion of prolactin (PL). Growth hormone and prolactin are produced by individual cells types (somatotrophs and mammotrophs, respectively) or by the same cells (somatomammotrophs [9]).Phylogenetically, GH and PL originate from the same precursor gene [6], which suggests common mechanisms of production and secretion of these hormones and their interaction at the paracrine and autocrine levels.Increased hormonal production and alterations of receptors associated with lowered inhibitory effect Endocrinology Research Center, Russian Academy of Medical Sciences, Moscow of hypothalamus or formation of abnormal relationships should be accompanied by pathological shifts in hormonal interactions and in the regulation of hormone production.In order to test this hypothesis we studied secretion of GH and PL by hypophyseal adenoma cells from acromegalic patients and the effects of somatoliberin (GH releasing factor) and somatostatin (GH inhibiting factor) and compared them with the effect of thyroliberin, a hypothalamic prolactotropic and thyrotropic regulator. MATERIALS AND METHODSHypophyseal tumor specimens were obtained upon surgery from 6 patients with acromegaly, treated with 0.25% trypsin, and homogenized. The procedure was described in detail elsewhere [3]. Cells were seeded (10 s cells/well) into 96-well plates (Flow), and cultures were grown until confluency in medium 199 containing 10% fetal calf serum and antibiotics at

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Activity of growth hormone-releasing peptide-2 in cultured human pituitary adenoma cells and its interaction with growth hormone-releasing hormone and somatostatin

et al. 1998

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Experiments on primary cultures of human pituitary adenoma cells producing growth hormone (GH) or GH and prolactin showed that similarly to GH-releasing hormone (GHRH) synthetic hexapeptide GH-releasing peptide-2 (GHRP) directly enhance secretion of GH but not of prolactin by human pituitary cells. The effect of various doses of GHRP and GHRH applied in combination was additive or slightly .synergistic in nature. Somatostatin inhibits secretion of GH induced by GHRP, GHRH, or their combination. A dissociation is found between the inhibitory effects of somatostatin on basal and stimulated GH secretion.

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Regulation of hormonal secretion in primary cell cultures of human somatotrophinomas

Cited by 4 publications

References 9 publications

Morphological and functional characteristics of human pituitary lactotrophic adenomas in cell cultures

Morphological and functional characteristics of human pituitary lactotrophic adenomas in cell cultures

Secretory reactions of cultured hypophyseal growth hormone-producing adenoma cells to somatoliberin and somatostatin

Activity of growth hormone-releasing peptide-2 in cultured human pituitary adenoma cells and its interaction with growth hormone-releasing hormone and somatostatin

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