Regulation of Hippo pathway components by FSH in testis

Sharma, Souvik Sen; Vats, Amandeep; Majumdar, Subeer S.

doi:10.1016/j.repbio.2019.01.003

Cited by 18 publications

(15 citation statements)

References 30 publications

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“…Nevertheless, the inverse correlation of vgll3a with yap1 in our data implies that these two cofactors could have somewhat opposite roles during development in different stages of cell differentiation processes, such as adipogenesis. And while Yap upregulation has been detected during mammalian puberty (Sen Sharma et al 2019;Zhang et al 2019), it is intriguing that vgll3a, not yap1, genetic variation is tightly associated with maturation timing in Atlantic salmon (Ayllon et al 2015;Barson et al 2015). Moreover, we observed that the expression levels of vgll3a and akap11a correlate positively, and that expression of akap11a further correlates negatively with yap1.…”

Section: Discussionmentioning

confidence: 59%

“…These genes encode Mst2 kinase needed to inactivate Yap and a transcription factor partner for Vgll3 and Yap, respectively. Accordingly, MST1/2 kinase genes, tead3 and also other major Hippo pathway members have been linked with maturation process both in Atlantic salmon (Christensen et al 2017;Kjaerner-Semb et al 2018) and other species (Lyu et al 2016;Sen Sharma et al 2019;Zhang et al 2019). Additionally, six6a correlated with three PKA regulatory subunit genes.…”

Section: Discussionmentioning

confidence: 93%

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Transcription Profiles of Age-at-Maturity-Associated Genes Suggest Cell Fate Commitment Regulation as a Key Factor in the Atlantic Salmon Maturation Process

Kurko

Debes

House

et al. 2020

G3 Genes|Genomes|Genetics

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Despite recent taxonomic diversification in studies linking genotype with phenotype, follow-up studies aimed at understanding the molecular processes of such genotype-phenotype associations remain rare. The age at which an individual reaches sexual maturity is an important fitness trait in many wild species. However, the molecular mechanisms regulating maturation timing processes remain obscure. A recent genome-wide association study in Atlantic salmon (Salmo salar) identified large-effect age-at-maturity-associated chromosomal regions including genes vgll3, akap11 and six6, which have roles in adipogenesis, spermatogenesis and the hypothalamic-pituitary-gonadal (HPG) axis, respectively. Here, we determine expression patterns of these genes during salmon development and their potential molecular partners and pathways. Using Nanostring transcription profiling technology, we show development- and tissue-specific mRNA expression patterns for vgll3, akap11 and six6. Correlated expression levels of vgll3 and akap11, which have adjacent chromosomal location, suggests they may have shared regulation. Further, vgll3 correlating with arhgap6 and yap1, and akap11 with lats1 and yap1 suggests that Vgll3 and Akap11 take part in actin cytoskeleton regulation. Tissue-specific expression results indicate that vgll3 and akap11 paralogs have sex-dependent expression patterns in gonads. Moreover, six6 correlating with slc38a6 and rtn1, and Hippo signaling genes suggests that Six6 could have a broader role in the HPG neuroendrocrine and cell fate commitment regulation, respectively. We conclude that Vgll3, Akap11 and Six6 may influence Atlantic salmon maturation timing via affecting adipogenesis and gametogenesis by regulating cell fate commitment and the HPG axis. These results may help to unravel general molecular mechanisms behind maturation.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 93%

Transcription Profiles of Age-at-Maturity-Associated Genes Suggest Cell Fate Commitment Regulation as a Key Factor in the Atlantic Salmon Maturation Process

Kurko

Debes

House

et al. 2020

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

show abstract

“…Nevertheless, the inverse correlation of vgll3a with yap1 in our data implies that these two cofactors could have somewhat opposite roles during development in different stages of cell differentiation processes, such as adipogenesis. And while Yap upregulation has been detected during mammalian puberty (36,37), it is intriguing that vgll3, not yap1, genetic variation is tightly associated with maturation timing in Atlantic salmon (6,66). Moreover, we observed that the expression levels of vgll3a and akap11a correlate positively, and that expression of akap11a further correlates negatively with yap1.…”

Section: Discussionmentioning

confidence: 60%

“…In line with cell fate regulation, it has been demonstrated earlier that Vgll3 may also have a role in tumor suppression in ovarian cancer (31). Furthermore, one of the Tead genes, tead3, and other major Hippo pathway members have been linked with maturation in Atlantic salmon (29,34) and other species (35)(36)(37) emphasizing the role of the Hippo pathway in the maturation process.…”

Section: Introductionmentioning

confidence: 78%

Transcription profiles of age-at-maturity-associated genes suggest cell fate commitment regulation as a key factor in the Atlantic salmon maturation process

Kurko

Debes

House

et al. 2019

Preprint

View full text Add to dashboard Cite

Despite recent taxonomic diversification in studies linking genotype with phenotype, follow-up studies aimed at understanding the molecular processes of such genotype-phenotype associations remain rare. The age at which an individual reaches sexual maturity is an important fitness trait in many wild species. However, the molecular mechanisms regulating maturation timing processes remain obscure. A recent genome-wide association study in Atlantic salmon (Salmo salar) identified large-effect age-at-maturity-associated chromosomal regions including genes vgll3, akap11 and six6, which have roles in adipogenesis, spermatogenesis and the hypothalamic-pituitary-gonadal (HPG) axis, respectively. Here, we determine expression patterns of these genes during salmon development and their potential molecular partners and pathways. Using Nanostring transcription profiling technology, we show development-and tissue-specific mRNA expression patterns for vgll3, akap11 and six6. Correlated expression levels of vgll3 and akap11, which have adjacent chromosomal location, suggests they may have shared regulation. Further, vgll3 correlating with arhgap6 and yap1, and akap11 with lats1 and yap1 suggests that Vgll3 and Akap11 take part in actin cytoskeleton regulation. Tissue-specific expression results indicate that vgll3 and akap11 paralogs have sexdependent expression patterns in gonads. Moreover, six6 correlating with slc38a6 and rtn1, and Hippo signaling genes suggests that Six6 could have a broader role in the HPG neuroendrocrine and cell fate commitment regulation, respectively. We conclude that Vgll3, Akap11 and Six6 may influence Atlantic salmon maturation timing via affecting on adipogenesis and gametogenesis by regulating cell fate commitment and the HPG axis. These results may help to unravel general molecular mechanisms behind maturation.

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“…Accumulating studies have indicated that various signaling pathways in SCs are implicated with spermatogenesis 27,28 . Until now, numerous signaling pathways have been found in Sertoli cells, including the androgensignaling pathway 29,30 , the AMP-activated protein kinase (AMPK) signaling pathway 31 , the follicle stimulating hormone (FSH)/adenylate cyclase/cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) signaling pathway 32 , the Hippo signaling pathway 33,34 , the intergrin mediated signaling pathway 4,35 , the Janus kinase/signal transducer and activator of transcription signaling pathway 36,37 , the mitogen-activated protein kinases (MAPK) signaling pathway [38][39][40][41][42][43][44] , the nuclear factor kappa B signaling pathway 45,46 , the nitric oxide/soluble guanylyl cyclase/cyclic guanosine monophosphate/ protein kinase G signaling pathway 47,48 , the Notch signaling pathway 49 , the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3k)/AKT serine/threonine kinase (Akt) signaling pathway 50,51 , the Sonic Hedgehog signaling pathway 52,53 , the transforming growth factor-β (TGF-β)/ Smad signaling pathway 54 , and the Wnt signaling pathway 55,56 (Table 1). Among all these signaling pathways, the TGF-β/Smad, AMPK, and MAPK signaling pathways have attracted much more attentions in the past decade.…”

Section: Introductionmentioning

confidence: 99%

Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis

2019

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The functions of Sertoli cells in spermatogenesis have attracted much more attention recently. Normal spermatogenesis depends on Sertoli cells, mainly due to their influence on nutrient supply, maintenance of cell junctions, and support for germ cells’ mitosis and meiosis. Accumulating evidence in the past decade has highlighted the dominant functions of the MAPK, AMPK, and TGF-β/Smad signaling pathways during spermatogenesis. Among these pathways, the MAPK signaling pathway regulates dynamics of tight junctions and adherens junctions, proliferation and meiosis of germ cells, proliferation and lactate production of Sertoli cells; the AMPK and the TGF-β/Smad signaling pathways both affect dynamics of tight junctions and adherens junctions, as well as the proliferation of Sertoli cells. The AMPK signaling pathway also regulates lactate supply. These signaling pathways combine to form a complex regulatory network for spermatogenesis. In testicular tumors or infertile patients, the activities of these signaling pathways in Sertoli cells are abnormal. Clarifying the mechanisms of signaling pathways in Sertoli cells on spermatogenesis provides new insights into the physiological functions of Sertoli cells in male reproduction, and also serves as a pre-requisite to identify potential therapeutic targets in abnormal spermatogenesis including testicular tumor and male infertility.

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Regulation of Hippo pathway components by FSH in testis

Cited by 18 publications

References 30 publications

Transcription Profiles of Age-at-Maturity-Associated Genes Suggest Cell Fate Commitment Regulation as a Key Factor in the Atlantic Salmon Maturation Process

Transcription Profiles of Age-at-Maturity-Associated Genes Suggest Cell Fate Commitment Regulation as a Key Factor in the Atlantic Salmon Maturation Process

Transcription profiles of age-at-maturity-associated genes suggest cell fate commitment regulation as a key factor in the Atlantic salmon maturation process

Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis

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