Regulation of heparin-binding epidermal growth factor-like growth factor mRNA levels by hypertrophic stimuli in neonatal and adult rat cardiac myocytes.

Perrella, Mark A.; Mäki, Tiina; Prasad, Sanjay; Pimental, David R.; Singh, Krishna; Takahashi, Nobuyuki; Yoshizumi, Masao; Alali, A; Higashiyama, Shigeki; Kelly, Ralph A.

doi:10.1016/s0021-9258(18)47123-2

Cited by 43 publications

(1 citation statement)

References 34 publications

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“…In our studies, the steady-state levels of 18S rRNA remained constant in response to various hormonal manipulations. Also, this is in keeping with recent reports recommending the use of rRNA gene expression as a preferred RNA-loading (internal) control for Northern blot analysis in which total RNA is used (Barbu & Dauty, 1989;Perrella et al, 1994;Yang & Tashjian, 1993;DeLeeuw et al, 1989;Bhatia et al, 1994). More widely used internal controls such as glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin were not employed in the current studies, since the steady-state levels of these mRNAs are known to be greatly influenced by hormones and growth factors (Alexander et al, 1988;Wadsworth et al, 1990;Bray et al, 1991;Sabath et al, 1990;Skinner et al, 1985;Amsterdam & Rotmensch, 1987;Amsterdam et al, 1989;Reaven et al, 1994).…”

Section: Methodssupporting

confidence: 81%

Regulation of Cholesterol Responsive Genes in Ovary Cells: Impact of Cholesterol Delivery Systems

et al. 1996

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The "selective" cholesterol uptake pathway represents a bulk pathway by which many steroidogenic cells internalize lipoprotein-delivered cholesteryl esters. In the current report, we question whether cholesteryl esters entering cells via this pathway are capable of governing standard cholesterol end product feedback repression mechanisms. Cultured rat ovary granulosa cells which utilize both the "selective" and "endocytic" pathways to internalize lipoprotein-derived cholesteryl esters were used as a model system. ApoE-free hHDL3 was used to deliver cholesteryl esters to the cells exclusively by the selective pathway; hLDL was used as a control ligand which when internalized by the endocytic pathway releases cholesteryl esters which subsequently regulate the expression of the B/E (LDL)-receptor, HMG CoA reductase, and acyl-CoA:cholesterol acyltransferase (ACAT). Whereas trophic hormone (Bt2cAMP) stimulation by itself increased the activity, mRNA, and protein levels of both B/E-receptor and HMG CoA reductase, pretreatment with either lipoprotein (adjusted for equal cholesterol ester content) down-regulated this expression. Linked with these lipoprotein-related changes was an increase in activity (though not gene expression) of ACAT. The level of change in mRNA levels, protein content, and activity for the examined regulatory proteins was essentially equivalent whether the lipoprotein provided to the cells was hLDL or hHDL3. Thus, similar signals appear to have been received by the cells despite differences in the uptake and processing of the ligand-derived cholesteryl esters, and these signals resulted in identical homeostatic responses by the cells.

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Section: Methodssupporting

confidence: 81%

Regulation of Cholesterol Responsive Genes in Ovary Cells: Impact of Cholesterol Delivery Systems

et al. 1996

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Rapid Signaling Pathways

Marı́n-Garcı́a

2011

Signaling in the Heart

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Contractile and cytoskeletal proteins in urinary bladder smooth muscle from rats treated with epidermal growth factor

et al. 1996

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Systemic treatment with epidermal growth factor (EGF) induces growth of all wall layers in the urinary tract of pigs and rats. The present study was initiated to describe morphological and biochemical changes in the bladder smooth muscle from rats treated with EGF for 4 weeks. Eight-week-old female Wistar rats were treated with subcutaneous injections of vehicle (n = 16) or EGF (n = 8, 150 micrograms/kg per day) for 4 weeks. After EGF treatment the bladders were increased in weight [74.4 +/- 0.4 vs 122.1 +/- 0.5 mg, P < 0.001 (mean +/- SEM)]. Sodium dedecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analyses of six bladders from each group revealed that the total amounts of actin, myosin and desmin were statistically significantly increased by 62%, 61% and 154%, respectively. The relative amounts of actin and myosin were unchanged whereas the desmin to actin ratio was significantly increased - as previously described in rat bladder smooth muscle hypertrophy. Light and electron microscopy of two bladders from each group revealed increased wall thickness involving all wall layers. The smooth muscle fibres at a midventral bladder location seemed only slightly hypertrophic-some degree of hyperplasia was therefore suspected. In conclusion, EGF treatment for 4 weeks induced a net synthesis of contractile and cytoskeletal proteins in the urinary bladder smooth muscle.

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Regulation of heparin-binding epidermal growth factor-like growth factor mRNA levels by hypertrophic stimuli in neonatal and adult rat cardiac myocytes.

Cited by 43 publications

References 34 publications

Regulation of Cholesterol Responsive Genes in Ovary Cells: Impact of Cholesterol Delivery Systems

Regulation of Cholesterol Responsive Genes in Ovary Cells: Impact of Cholesterol Delivery Systems

Rapid Signaling Pathways

Contractile and cytoskeletal proteins in urinary bladder smooth muscle from rats treated with epidermal growth factor

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