Regulation of heat shock genes in isolated hepatocytes from an Antarctic fish,<i>Trematomus bernacchii</i>

Buckley, Bradley A.; Place, Sean P.; Hofmann, Gretchen E.

doi:10.1242/jeb.01219

Cited by 120 publications

(71 citation statements)

References 53 publications

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“…1B and 2Bi) in D. mawsoni strongly indicates a constitutive system-wide need for mitigating cold denaturation of proteins (23) in the face of chronic cold. It supports the reported constitutive HSP70 expression and loss of HSP70 inducibility in some notothenioids (24). The augmented need for cellular protein homeostasis is also indicated in the upregulation of genes in ubiquitin-dependent protein degradation pathways (Fig.…”

Section: Resultssupporting

confidence: 86%

Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish

Chen

Cheng

Zhang

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

225

214

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The antifreeze glycoprotein-fortified Antarctic notothenioid fishes comprise the predominant fish suborder in the isolated frigid Southern Ocean. Their ecological success undoubtedly entailed evolutionary acquisition of a full suite of cold-stable functions besides antifreeze protection. Prior studies of adaptive changes in these teleost fishes generally examined a single genotype or phenotype. We report here the genome-wide investigations of transcriptional and genomic changes associated with Antarctic notothenioid cold adaptation. We sequenced and characterized 33,560 ESTs from four tissues of the Antarctic notothenioid Dissostichus mawsoni and derived 3,114 nonredundant protein gene families and their expression profiles. Through comparative analyses of same-tissue transcriptome profiles of D. mawsoni and temperate/tropical teleost fishes, we identified 177 notothenioid protein families that were expressed many fold over the latter, indicating cold-related up-regulation. These up-regulated gene families operate in protein biosynthesis, protein folding and degradation, lipid metabolism, antioxidation, antiapoptosis, innate immunity, choriongenesis, and others, all of recognizable functional importance in mitigating stresses in freezing temperatures during notothenioid life histories. We further examined the genomic and evolutionary bases for this expressional up-regulation by comparative genomic hybridization of DNA from four pairs of Antarctic and basal non-Antarctic notothenioids to 10,700 D. mawsoni cDNA probes and discovered significant to astounding (3-to >300-fold, P < 0.05) Antarctic-specific duplications of 118 protein-coding genes, many of which correspond to the up-regulated gene families. Results of our integrative tripartite study strongly suggest that evolution under constant cold has resulted in dramatic genomic expansions of specific protein gene families, augmenting gene expression and gene functions contributing to physiological fitness of Antarctic notothenioids in freezing polar conditions. cold adaptation ͉ comparative genomics ͉ gene duplication ͉ genome evolution ͉ retrotransposon

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Section: Resultssupporting

confidence: 86%

Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish

Chen

Cheng

Zhang

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

225

214

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“…Hsp expression is strongly up-regulated during dormancies ranging from encystment in the brine shrimp, Artemia franciscana (33), and the quiescent stages of the freshwater sponge, Spongilla lacustris (34), to mammalian hibernation (35,36), and small Hsps are up-regulated during dormancy in a number of plant tissues including seeds (37-39), parenchyma cells (40), and bark tissue (41). Additionally, polar organisms including larvae of the terrestrial Antarctic midge, Belgica antarctica (42), an Antarctic fish, Trematomus bernacchii, (43), and an Antarctic marine ciliate, Euplotes focardii (44), continuously express Hsps. Thus, Hsps appear to be widely used by organisms subjected to cold environments.…”

Section: Resultsmentioning

confidence: 99%

Up-regulation of heat shock proteins is essential for cold survival during insect diapause

Rinehart

Yocum

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

463

400

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Diapause, the dormancy common to overwintering insects, evokes a unique pattern of gene expression. In the flesh fly, most, but not all, of the fly's heat shock proteins (Hsps) are up-regulated. The diapause up-regulated Hsps include two members of the Hsp70 family, one member of the Hsp60 family (TCP-1), at least four members of the small Hsp family, and a small Hsp pseudogene. Expression of an Hsp70 cognate, Hsc70, is uninfluenced by diapause, and Hsp90 is actually down-regulated during diapause, thus diapause differs from common stress responses that elicit synchronous up-regulation of all Hsps. Up-regulation of the Hsps begins at the onset of diapause, persists throughout the overwintering period, and ceases within hours after the fly receives the signal to reinitiate development. The up-regulation of Hsps appears to be common to diapause in species representing diverse insect orders including Diptera, Lepidoptera, Coleoptera, and Hymenoptera as well as in diapauses that occur in different developmental stages (embryo, larva, pupa, adult). Suppressing expression of Hsp23 and Hsp70 in flies by using RNAi did not alter the decision to enter diapause or the duration of diapause, but it had a profound effect on the pupa's ability to survive low temperatures. We thus propose that up-regulation of Hsps during diapause is a major factor contributing to cold-hardiness of overwintering insects.cold tolerance ͉ overwintering ͉ stress proteins ͉ RNAi

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“…In our study, the mean values ranged from 0.41 to 0.48 in both sexes (Table 1). The lower values in our samples may reflect a poorer physiological condition of the sharks evaluated in the present study, due to stress factors such as the physiological attrition derived from being fished and captured, nutritional status, and the time elapsed after digesting the last prey (Mendoza-Alfaro et al, 2002;Buckley et al, 2004). Kieffer (2000) reported that an inevitable consequence of shark fishing is the extreme muscular fatigue of the fish.…”

Section: __________________mentioning

confidence: 75%

Oxidative stress and RNA DNA ratio following longline capture in the silky shark Carchahrinus falciformis (Muller and Henle, 1839)

Cruz‐Ramírez

Liñán‐Cabello

Tavares

et al. 2017

lajar

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ABSTRACT. This study used transcriptomics tracers and measurements of oxidative stress to examine the effect of capture by longline on the silky shark Carcharhinus falciformis. From February 2010 to May 2012, C. falciformis individuals captured in the eastern Pacific Ocean were initially examined for sex, degree of maturity, and morphometric data. Samples were then taken from muscle tissue to determine RNA, DNA, and protein concentrations as well as RNA/DNA and protein/DNA ratios. The levels of three oxidative stress indicators [superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA)] were determined in muscle. The analysis of the 159 specimens (93 juveniles and 66 adults) showed low RNA/DNA ratios at the different stages of maturity. This basal level of transcriptomics capacity may have been caused by the physiological stress resulting from capture. In juveniles, the low RNA/DNA and protein/DNA ratios were associated with low levels of cellular damage related to oxidative stress, whereas in adults the level of cell damage due to oxidative stress was high, especially in older females. This pattern indicated decreased antioxidant response capacity with increasing age in elasmobranchs.

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Regulation of heat shock genes in isolated hepatocytes from an Antarctic fish,Trematomus bernacchii

Cited by 120 publications

References 53 publications

Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish

Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish

Up-regulation of heat shock proteins is essential for cold survival during insect diapause

Oxidative stress and RNA DNA ratio following longline capture in the silky shark Carchahrinus falciformis (Muller and Henle, 1839)

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