Regulation of heat shock factor in Schizosaccharomyces pombe more closely resembles regulation in mammals than in Saccharomyces cerevisiae.

Gallo, G J; Schuetz, Thomas; Kingston, Robert E.

doi:10.1128/mcb.11.1.281

Cited by 63 publications

(48 citation statements)

References 32 publications

(45 reference statements)

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“…In addition, HA-SpHSF was able to bind various HSE types in a heat-inducible manner. This is consistent with the previous observation that, unlike ScHsf1, SpHSF needs heat shock to binds to the HSE (34,44).…”

Section: Binding Of Schsf1 To Target Genes That Lack An Apparentsupporting

confidence: 82%

Interaction between Heat Shock Transcription Factors (HSFs) and Divergent Binding Sequences

Sakurai

Takemori

2007

Journal of Biological Chemistry

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The target genes of the heat shock transcription factor (HSF) contain a cis-acting sequence, the heat shock element (HSE), which consists of multiple inverted repeats of the sequence 5-nGAAn-3. Using data acquired in this and a previous study, we have identified the HSEs in 59 of 62 target genes of Saccharomyces cerevisiae Hsf1. The Hsf1 protein recognizes continuous and discontinuous repeats of the nGAAn unit; the nucleotide sequences and configuration of the units diverge slightly among functional HSEs. When Schizosaccharomyces pombe HSF was expressed in S. cerevisiae cells, heat shock induced S. pombe HSF to bind to various HSE types, which properly activated transcription from almost all target genes, suggesting that the S. pombe genome also contains divergent HSEs. Human HSF1 induced the heat shock response via HSEs with continuous units in S. cerevisiae cells but failed to do so via HSEs with discontinuous units. Binding of human HSF1 to the discontinuous type of HSE was observed in vitro but was significantly inhibited in vivo. These results show that human HSF1 recognizes HSEs in a slightly different way than yeast HSFs and suggest that the configuration of the unit is an important determinant for HSF-HSE interactions.

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Section: Binding Of Schsf1 To Target Genes That Lack An Apparentsupporting

confidence: 82%

Interaction between Heat Shock Transcription Factors (HSFs) and Divergent Binding Sequences

Sakurai

Takemori

2007

Journal of Biological Chemistry

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“…Approximately 1.8 x 1010 S3 HeLa cells (30 liters of culture) growing in Joklik's MEM (with 5% horse serum) were heat shocked at 430C for 1 hr and nuclear extracts were prepared (14). HSF was purified essentially as described (15) with the following differences: Nonidet P-40 was 0.1% in all buffers, n-octyl glucoside was 5 mM in load buffer B, and affinity columns were washed with load buffer B containing 0.8 M KCl and eluted with load buffer B adjusted to 2.0 M KCI and 0.1 M MgCl2.…”

Section: Methodsmentioning

confidence: 99%

Isolation of a cDNA for HSF2: evidence for two heat shock factor genes in humans.

Schuetz

Gallo

Sheldon

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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“…The ability of S. pombe HSF to bind to the HSE significantly increases following heat shock, and this increase occurs in the presence of cycloheximide, suggesting that the regulation is posttranslational (10). In addition to this inducible binding activity, an activity that constitutively binds to the HSE has been reported in both humans and S. pombe (26,46).…”

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confidence: 96%

Heat shock factor is required for growth at normal temperatures in the fission yeast Schizosaccharomyces pombe.

1993

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Schizosaccharomyces pombe is becoming an increasingly useful organism for the study of cellular processes, since in certain respects, such as the cell cycle and splicing, it is similar to metazoans. Previous biochemical studies have shown that the DNA binding ability of S. pombe heat shock factor (HSF) is fully induced only under stressed conditions, in a manner similar to that of Drosophila melanogaster and humans but differing from the constitutive binding by HSF in the budding yeasts. We report the isolation of the cDNA and gene for the HSF from S. pombe. S. pombe HSF has a domain structure that is more closely related to the structure of human and D. The mechanism by which eukaryotic cells respond to heat shock is highly conserved (reviewed in references 21 and 24). This response, which also protects cells from numerous other forms of stress, is presumed to have been conserved throughout evolution because the ability of a cell to survive stress is critical to its viability in a natural setting. A major aspect of the heat shock response is the transcriptional induction of the genes that encode the heat shock proteins. This induction is regulated by heat shock factor (HSF), a transcription factor whose DNA binding site (the heat shock element [HSE]) is similar in all studied eukaryotes. This binding site is found in the promoter regions of heat shock genes and consists of inverted repeats of the sequence nGAAn (3,27,45). Binding of HSF to this site results in 20-to 1,000-fold transcriptional stimulation, depending on the specific promoter and cell type. The potent transcriptional stimulatory properties of HSF, the conserved nature of the heat shock response, and the importance of this response to cellular physiology have caused HSF to be an intensively studied factor.The regulation of HSF following stress has been compared in several eukaryotic organisms. In the budding yeast Saccharomyces cerevisiae, HSF is constitutively bound to the HSE, and heat shock increases the degree of transcriptional stimulation attributed to HSF (14,37,39,40). This increase in transcriptional stimulatory ability is believed to be regulated by posttranslational modification of S. cerevisiae HSF (37, 40). There is a correlation between increased phosphorylation of the molecule and increased transcription of the heat shock genes, and it has therefore been proposed that phosphorylation might regulate transcriptional stimulation * Corresponding author. t Present address: Immunogen, Cambridge, MA 02139.by this HSF. Study of a related budding yeast, Kluyveromyces lactis, identified a conserved seven-amino-acid sequence in HSF that is necessary for appropriate regulation of the transcriptional stimulatory properties of S. cerevisiae and K lactis HSFs (15). The precise role that this seven-amino-acid sequence plays in regulating HSF has not been elucidated. Budding yeast HSF is one of the factors responsible for maintaining basal (nonstressed) expression from certain heat shock promoters, in part because of its ability to constitutively bin...

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Regulation of heat shock factor in Schizosaccharomyces pombe more closely resembles regulation in mammals than in Saccharomyces cerevisiae.

Cited by 63 publications

References 32 publications

Interaction between Heat Shock Transcription Factors (HSFs) and Divergent Binding Sequences

Interaction between Heat Shock Transcription Factors (HSFs) and Divergent Binding Sequences

Isolation of a cDNA for HSF2: evidence for two heat shock factor genes in humans.

Heat shock factor is required for growth at normal temperatures in the fission yeast Schizosaccharomyces pombe.

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