Regulation of glucose catabolism in Thiobacillus A2 grown in the chemostat under dual limitation by succinate and glucose

Wood, Ann P.; Kelly, Donovan P.

doi:10.1007/bf00422311

Cited by 8 publications

(3 citation statements)

References 17 publications

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“…Growth yields of around 100 g dry wt (mol glucose)-' , 1980bSmith et al, 1980) for Thiobacillus A2 indicate that up to 60% of available glucose-carbon may be assimilated in heterotrophic cultures. Using medium containing [U-14C ]glucose, steady state incorporation of glucose-carbon was found to be dependent on growth rate.…”

Section: Contribution Of Glucose To Carbon Metabolism In Mixotrophic mentioning

confidence: 99%

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Mixotrophic Growth of Thiobacillus A2 in Chemostat Culture on Formate and Glucose

Wood

Kelly

1981

Microbiology

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In aerobic chemostat culture Thiobacillus A2-GFI grew autotrophically on formate and heterotrophically on glucose with maximum specific growth rates (,urn,,) of 0.21 and 0.33 h-l, respectively. At dilution rates of 0.1 and 0.18 h-l, it grew mixotrophically on formate + glucose mixtures, completely consuming both substrates. Ribulose-1,5-bisphosphate carboxylase and formate dehydrogenase were present at high specific activity in autotrophic and mixotrophic cultures, but were repressed in cultures on glucose alone. A greater proportion of added glucose was assimilated in mixotrophic culture than in heterotrophic culture. Raising the dilution rate of a mixotrophic culture from 0.1 or 0.18 to 0.3 h-' resulted in washout (with an apparent p,,, for mixotrophic growth of 0.25 h-l) and the establishment of a culture dependent on glucose for growth. Growth yields on formate and glucose were, respectively, 3 -3 and 100 g dry wt (mol substrate consumed)-'. Steady state biomass production in mixotrophic culture indicated additive growth yields. The biomass produced in cultures on formate + glucose at a dilution rate of 0-3 h-l suggested that growth only occurred on glucose, but organisms still contained high activities of ribulose-1,5-bisphosphate carboxylase and formate dehydrogenase. At a formate :glucose ratio (mM) of 100 : 1, some formate was oxidized and CO, was fixed, but formate was not used when this ratio was 5 0 : 5 . Formate-glucose mixotrophy benefits Thiobacillus A2-GFI when substrates are limited at low growth rates (<,urnax for formate), but is characterized by a pmax below that possible on glucose. Physiological behaviour at high growth rates was influenced by the formate :glucose ratio, resulting under some conditions, at least, in loss of mixotrophy and the establishment of heterotrophic growth.

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Section: Contribution Of Glucose To Carbon Metabolism In Mixotrophic mentioning

confidence: 99%

“…Cultures (750 ml) were grown at 30 OC in an LH modular type series 500 fermenter (LH Engineering, Slough, Bucks) as described previously (Wood & Kelly, 1980b), with air flow rates of 150 or 250 ml min-' and an impeller speed of 750 rev. min-'.…”

Section: E T H O D Smentioning

confidence: 99%

Mixotrophic Growth of Thiobacillus A2 in Chemostat Culture on Formate and Glucose

Wood

Kelly

1981

Microbiology

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“…Cultures (750 ml) were grown at 30°C in an LH modular types series 500 fermenter (LH Engineering, Slough, Bucks) as described previously [9] with an impeller speed of 750 rev./min and gas flow rates as stated in RESULTS. The culture was maintained at pH 7.8 by automatic titration with 5 M NaOH, and alkali consumption recorded throughout.…”

Section: Continuous Culturementioning

confidence: 99%

Autotrophic, mixotrophic and heterotrophic growth with denitrification by Thiobacillus A2 under anaerobic conditions

Wood¹

1983

FEMS Microbiology Letters

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Sulfur-oxidizing Bacteria: A Novel Bioinoculant for Sulfur Nutrition and Crop Production

Anandham

Gandhi

Senthilkumar

et al. 2011

Bacteria in Agrobiology: Plant Nutrient Management

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Regulation of glucose catabolism in Thiobacillus A2 grown in the chemostat under dual limitation by succinate and glucose

Cited by 8 publications

References 17 publications

Mixotrophic Growth of Thiobacillus A2 in Chemostat Culture on Formate and Glucose

Mixotrophic Growth of Thiobacillus A2 in Chemostat Culture on Formate and Glucose

Autotrophic, mixotrophic and heterotrophic growth with denitrification by Thiobacillus A2 under anaerobic conditions

Sulfur-oxidizing Bacteria: A Novel Bioinoculant for Sulfur Nutrition and Crop Production

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