Regulation of gene expression directed by the long terminal repeat of the feline immunodeficiency virus

Sparger, Ellen E.; Shacklett, Barbara L.; Renshaw-Gegg, Lisa; Barry, Peter A.; Pedersen, Niels C; Elder, John H.; Luciw, Paul A.

doi:10.1016/0042-6822(92)90305-9

Cited by 93 publications

(124 citation statements)

References 46 publications

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“…In accord with previous studies (Sparger et al, 1992;Kawaguchi et al, 1992), we found high basal expression but no significant trans-activation of reporter genes linked to the full-length FIV LTR. However, truncated promoter constructs with low basal expression showed significant restoration of activity by FIV in trans, suggesting that trans-activation of the FIV promoter may be operative in some cellular environments.…”

Section: Introductionsupporting

confidence: 81%

“…Previous studies have shown that the full-length LTR of FIV is only very weakly trans-activated, if at all, by cotransfection with FIV clones or subgenomic components (Sparger et al, 1992). However, the possibility remained that trans-activation might be masked by the high basal activity of the FIV LTR in established cell lines.…”

Section: Trans-activation Of the Fiv-uk8 Ltr In Crfk Cellsmentioning

confidence: 72%

“…The effect was slight in AH927 and CrFK cells but much more marked in HeLa cells. FIV 5' deletion mutants of Japanese and U.S. isolates showed a similar slight increase in fcwfo4 (Kawaguchi et al, 1992) and CrFK cells but a much larger effect in G355-5 brain-derived cells (Sparger et al, 1992). It is interesting to note that the relevant area of the LTR includes a purine-rich sequence (-135/-155) and that negative regulatory activity has been mapped to similar purine-rich binding sites in the human immunodeficiency virus and interleukin 2 gene promoters (Rosen et al, 1985;Siekevitz et al, 1987;Shaw et al, 1988).…”

Section: Discussionmentioning

confidence: 86%

“…The mutations appeared to have some selective phenotypic consequences since the AP-1 mutation had a greater effect on protein kinase C-mediated reponses but the ATF mutation was more deleterious to cAMP responses mediated by protein kinase A (Sparger et al, 1992). In view of the cross-competition between AP-1 and ATF motifs observed here, and the recognition of AP-1 and ATF by an overlapping set of factors (Hai et al, 1988), it might be considered that one intact site could mediate both responses, albeit less efficiently.…”

Section: Discussionmentioning

confidence: 99%

“…Previous reports have highlighted a number of motifs in the FIV LTR that are potential sites for protein binding (Olmsted et al, 1989b;Talbott et al, 1989;Phillips et al, 1990;Miyazawa et al, 1991) and mutational analysis has shown that putative AP-1 and ATF sites are required for full basal promoter function (Sparger et al, 1992). However, there has until now been no direct analysis of nuclear proteins interacting with the FIV LTR.…”

Section: Introductionmentioning

confidence: 99%

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Cis- and trans-regulation of feline immunodeficiency virus: identification of functional binding sites in the long terminal repeat

Thompson

Elder

Neil

1994

Journal of General Virology

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Section: Introductionsupporting

confidence: 81%

Section: Trans-activation Of the Fiv-uk8 Ltr In Crfk Cellsmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cis- and trans-regulation of feline immunodeficiency virus: identification of functional binding sites in the long terminal repeat

Thompson

Elder

Neil

1994

Journal of General Virology

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Efficient Transduction of Nondividing Cells by Optimized Feline Immunodeficiency Virus Vectors

et al. 2000

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Second- and third-generation three-plasmid vector systems, termed FELIX, were constructed from feline immunodeficiency virus (FIV). To enhance vector production, the weak FIV long terminal repeat promoter was replaced with the human cytomegalovirus enhancer/promoter. To construct a minimal system in which Gag-Pol was the only viral protein present, the cytoplasmic transport element was used in place of the FIV Rev-RRE system to facilitate nuclear export of Gag-Pol and the transfer vector. Unconcentrated vector titers routinely exceeded 1 x 10(6) IU/mL for most constructs tested. Second- and optimized third-generation vectors were capable of efficiently infecting G1/S- and G2/M-arrested cells. FIV-based FELIX vectors transduced human dendritic cells, hepatocytes, and aortic smooth muscle with efficiencies similar to that of a control 3T3 cell line. All three of these primary cell types were transducible by both the second- and third-generation FELIX vectors, demonstrating that FIV Gag-Pol alone contains the determinants necessary for transduction of primary cells. In cross-packaging tests, we observed that HIV Gag-Pol does not substantially package FIV vectors; consequently, use of such vectors in human immunodeficiency virus-infected cells should not lead to efficient mobilization of the inserted gene. Thus, this FIV-based vector system offers high efficiency and stable delivery of genes to numerous nondividing and primary cell types, opening new avenues for biological inquiry into normal human cells.

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Proviral Organization and Sequence Analysis of Feline Immunodeficiency Virus Isolated from a Pallas' Cat

et al. 1997

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The nucleotide sequence and genomic organization have been determined for a highly cytopathic feline immunodeficiency virus (FIV) isolated from a Pallas' cat. The 9747-bp provirus of this virus, FIV-Oma, has typical lentivirus organization with LTRs, gag, pol, and env open reading frames (ORFs), putative vif and rev ORFs, and an ORF similar to ORF2/ORFA of domestic cat FIV isolates. Although the FIV-Oma provirus is 300 to 600 bp longer than other FIV proviruses, these additional bases are distributed throughout the genome. Phylogenetic analysis of a conserved region of the pol gene suggests that FIV-Oma is more closely related to some of the puma and lion lentiviruses than it is to domestic cat FIV isolates; however, many regions of the genome exhibit extensive nucleotide sequence divergence. None of the eight molecular proviral clones isolated from a genomic library are infectious, but we have constructed an infectious, cytopathic clone of FIV-Oma from subcloned and PCR-amplified fragments of these proviral clones. This clone will be useful for identifying the genetic determinants of FIV-Oma's biological activities.

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Regulation of gene expression directed by the long terminal repeat of the feline immunodeficiency virus

Cited by 93 publications

References 46 publications

Cis- and trans-regulation of feline immunodeficiency virus: identification of functional binding sites in the long terminal repeat

Cis- and trans-regulation of feline immunodeficiency virus: identification of functional binding sites in the long terminal repeat

Efficient Transduction of Nondividing Cells by Optimized Feline Immunodeficiency Virus Vectors

Proviral Organization and Sequence Analysis of Feline Immunodeficiency Virus Isolated from a Pallas' Cat

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