Regulation of Gap Junction Intercellular Communication in Primary Canine Lens Epithelial Cells: Role of Protein Kinase C

Long, Amy C.; Colitz, Carmen M. H.; Bomser, Joshua A.

doi:10.1080/02713680601186714

Cited by 11 publications

(7 citation statements)

References 39 publications

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“…As in these other systems, treatment of epithelial cells with phorbol esters (such as TPA) leads to a decrease in intercellular communication (81,131,159) associated with increased Cx43 phosphorylation (detectable by 32 P incorporation) (131). Several mechanisms have been proposed for the TPAinduced decrease in intercellular communication.…”

Section: Effects Of Pkc Activationmentioning

confidence: 96%

Oxidative Stress, Lens Gap Junctions, and Cataracts

Berthoud

Beyer

2009

Antioxidants & Redox Signaling

213

141

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The eye lens is constantly subjected to oxidative stress from radiation and other sources. The lens has several mechanisms to protect its components from oxidative stress and to maintain its redox state, including enzymatic pathways and high concentrations of ascorbate and reduced glutathione. With aging, accumulation of oxidized lens components and decreased efficiency of repair mechanisms can contribute to the development of lens opacities or cataracts. Maintenance of transparency and homeostasis of the avascular lens depend on an extensive network of gap junctions. Communication through gap junction channels allows intercellular passage of molecules (up to 1 kDa) including antioxidants. Lens gap junctions and their constituent proteins, connexins (Cx43, Cx46, and Cx50), are also subject to the effects of oxidative stress. These observations suggest that oxidative stress-induced damage to connexins (and consequent altered intercellular communication) may contribute to cataract formation. Antioxid. Redox Signal. 11, 339-353. 339The Eye Lens

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Section: Effects Of Pkc Activationmentioning

confidence: 96%

Oxidative Stress, Lens Gap Junctions, and Cataracts

Berthoud

Beyer

2009

Antioxidants & Redox Signaling

213

141

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“…20) We had previously reported that treatment of PMA (10 nM) augmented MMP-9 activity in the human lens epithelial cell line, SRA01/04. 17) Therefore, we examined the effect of KPMFs on the PMA-mediated activities of gelatinase through the analysis of conditioned media from KPMFs-treated cells by gelatin zymography. As shown in Fig.…”

Section: Effects Of Polymethoxylated Flavones Isolated From K Parvifmentioning

confidence: 99%

Potential Therapeutic Agents, Polymethoxylated Flavones Isolated from <i>Kaempferia parviflora</i> for Cataract Prevention through Inhibition of Matrix Metalloproteinase-9 in Lens Epithelial Cells

Miyata

Tatsuzaki²,

Yang³

et al. 2019

Biological & Pharmaceutical Bulletin

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Natural flavonoids have powerful antioxidant activity and have been reported to show promising protective effects against cataracts. The plant Kaempferia parviflora (K. parviflora) is indigenous to southeast Asia, including Thailand, and typically contains polymethoxylated flavones. The flavones in K. parviflora are reported to have various biological properties. Recently, polymethoxylated flavones of K. parviflora (KPMFs) were shown to have potent Sirtuin 1 enzyme-stimulating and anti-glycation activities that led to the suppression of cataract formation. Matrix metalloproteinases (MMPs) are upregulated in several pathologic ocular diseases, including cataracts, and have been established as an attractive target for the prevention and/or treatment of specific cataract phenotypes, such as anterior subcapsular cataract (ASC) and posterior capsular opacification (PCO). In the present study, we investigated the effect of KPMFs on MMP (gelatinase) activity in the human lens epithelial cell line, SRA01/04. We demonstrated that KPMFs inhibited the phorbol ester-induced MMP-9 activity and the mRNA expression through the suppression of mitogen-activated protein kinases (MAPKs) phosphorylation in human lens epithelial cells; 5,7-dimethoxyflavone was found to exert the most potent inhibition, but 3,5,7,4′-tetramethoxyflavone and 3,5,7,3′,4′-pentamethoxyflavone also resulted in considerable inhibition. Our results suggested that the consumption of PMFs isolated from K. parviflora, may be an effective strategy to delay the development of cataracts, such as ASC and PCO.

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“…In lens epithelial cells, Cx43 is phosphorylated by PKC [198]. In a rabbit lens epithelail cell line [N/N1003A] it has been reported that Cx43 phosphorylation by PKC-gamma is important to prevent the spread of apoptosis in lenses under oxidative stress [199] due to a disassembly of gap junction plaques [200,201].…”

Section: Connexins and Cataractsmentioning

confidence: 99%

“…In a rabbit lens epithelail cell line [N/N1003A] it has been reported that Cx43 phosphorylation by PKC-gamma is important to prevent the spread of apoptosis in lenses under oxidative stress [199] due to a disassembly of gap junction plaques [200,201]. A decrease of gap junction communication induced by TPA [a PKC activator] was also observed in bovine [202] and canine [203] lens epithelial cells, where the zonula occludens protein-1 [ZO-1] appears to be essential for gap junction disassembly induced by PKC-gamma [204]. This TPA induced-phosphorylation was associated with resistance to degradation by a proteosome dependent pathway [205], and stimulate the interactions PKC-gamma with Cx43 and Cav-1 [206].…”

Section: Connexins and Cataractsmentioning

confidence: 99%