Regulation of flowering time in chrysanthemum by the R2R3 MYB transcription factor CmMYB2 is associated with changes in gibberellin metabolism

Zhu, Li; Guan, Yunxiao; Liu, Yanan; Zhang, Zhaohe; Jaffar, Muhammad Abuzar; Song, Aiping; Chen, Sumei; Jiang, Jiafu; Chen, Fadi

doi:10.1038/s41438-020-0317-1

Cited by 34 publications

(17 citation statements)

References 43 publications

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“…Transcription factors (TFs) regulate plant growth and development through various signal transduction pathways [ 34 ]. Combined with previous studies, multiple flowering-related TFs were detected in the DEGs, such as bHLH, MADS-box, MYB and WRKY [ 35 , 36 , 37 ]. Noteworthy, ERF and NAC TFs were marked in top three in the three comparisons ( Figure 3 b), which used to be involved into the flowering process especially the regulation of floral organ development [ 38 , 39 ].…”

Section: Resultssupporting

confidence: 57%

Genetic and Transcriptomic Analysis Reveal the Molecular Basis of Photoperiod-Regulated Flowering in Xishuangbanna Cucumber (Cucumis sativus L. var. xishuangbannesis Qi et Yuan)

Tian

Jahn²,

Qin

et al. 2021

Genes

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Xishuangbanna (XIS) cucumber (Cucumis sativus L. var. xishuangbannesis Qi et Yuan), is a botanical variety of cucumber cultivars native to southwest China that possesses excellent agronomic traits for cucumber improvement. However, breeding utilization of XIS cucumber is limited due to the current poor understanding of its photoperiod-sensitive flowering characteristics. In this study, genetic and transcriptomic analysis were conducted to reveal the molecular basis of photoperiod-regulated flowering in XIS cucumber. A major-effect QTL locus DFF1.1 was identified that controls the days to first flowering (DFF) of XIS cucumbers with a span of 1.38 Mb. Whole-genome re-sequencing data of 9 cucumber varieties with different flowering characteristics in response to photoperiod suggested that CsaNFYA1 was the candidate gene of DFF1.1, which harbored a single non-synonymous mutation in its fifth exon. Transcriptomic analysis revealed the positive roles of auxin and ethylene in accelerating flowering under short-day (SD) light-dark cycles when compared with equal-day/night treatment. Carbohydrate storage and high expression levels of related genes were important reasons explaining early flowering of XIS cucumber under SD conditions. By combining with the RNA-Seq data, the co-expression network suggested that CsaNFYA1 integrated multiple types of genes to regulate the flowering of XIS cucumber. Our findings explain the internal regulatory mechanisms of a photoperiodic flowering pathway. These findings may guide the use of photoperiod shifts to promote flowering of photoperiod-sensitive crops.

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Section: Resultssupporting

confidence: 57%

Genetic and Transcriptomic Analysis Reveal the Molecular Basis of Photoperiod-Regulated Flowering in Xishuangbanna Cucumber (Cucumis sativus L. var. xishuangbannesis Qi et Yuan)

Tian

Jahn²,

Qin

et al. 2021

Genes

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“…In Arabidopsis , ectopic expression of the full-length JAZ1 cDNA did not lead to an early flowering phenotype; however, 35Spro:SlJAZ2 transgenic plants flowered one week earlier than the wild type 27 . Furthermore, with overexpression of the R2R3 MYB transcription factor CmMYB2 in Arabidopsis and chrysanthemum, the transgenic plants exhibited an opposite phenotype in terms of flowering time 50 . These phenomena probably result from the differences in the sequence and structure of proteins in different species, which generate diverse protein characteristics and lead to the formation of different dimers with different proteins, which further participate in diverse regulatory pathways.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of the CmJAZ1-like gene delays flowering in Chrysanthemum morifolium

Guan

Ding

Jiang

et al. 2021

Horticulture Research

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Chrysanthemum (Chrysanthemum morifolium) is one of the four major cut-flower plants worldwide and possesses both high ornamental value and cultural connotation. As most chrysanthemum varieties flower in autumn, it is costly to achieve annual production. JAZ genes in the TIFY family are core components of the jasmonic acid (JA) signaling pathway; in addition to playing a pivotal role in plant responses to defense, they are also widely implicated in regulating plant development processes. Here, we characterized the TIFY family gene CmJAZ1-like from the chrysanthemum cultivar ‘Jinba’. CmJAZ1-like localizes in the nucleus and has no transcriptional activity in yeast. Tissue expression pattern analysis indicated that CmJAZ1-like was most active in the root and shoot apex. Overexpressing CmJAZ1-like with Jas domain deletion in chrysanthemum resulted in late flowering. RNA-Seq analysis of the overexpression lines revealed some differentially expressed genes (DEGs) involved in flowering, such as the homologs of the flowering integrators FT and SOC1, an FUL homolog involved in flower meristem identity, AP2 domain-containing transcription factors, MADS box genes, and autonomous pathway-related genes. Based on KEGG pathway enrichment analysis, the differentially transcribed genes were enriched in carbohydrate metabolic and fatty acid-related pathways, which are notable for their role in flowering in plants. This study preliminarily verified the function of CmJAZ1-like in chrysanthemum flowering, and the results can be used in molecular breeding programs aimed at flowering time regulation of chrysanthemum.

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“…GRE was abundant in ClWRKY , an important hormone associated with flowering induction in chrysanthemum ( Dong et al, 2017 ). CmBBX24 regulates flowering in chrysanthemum in conjunction with gibberellin synthesis ( Zhu et al, 2020 ). In addition, the key recognition site for the WRKY gene, the W-box containing the TGAC sequence, was observed in most ClWRKY members.…”

Section: Discussionmentioning

confidence: 99%

Characterization of WRKY Gene Family in Whole-Genome and Exploration of Flowering Improvement Genes in Chrysanthemum lavandulifolium

Khan

Kang

et al. 2022

Front. Plant Sci.

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Chrysanthemum is a well-known ornamental plant with numerous uses. WRKY is a large family of transcription factors known for a variety of functions ranging from stress resistance to plant growth and development. Due to the limited research on the WRKY family in chrysanthemums, we examined them for the first time in Chrysanthemum lavandulifolium. A total of 138 ClWRKY genes were identified, which were classified into three groups. Group III in C. lavandulifolium contains 53 members, which is larger than group III of Arabidopsis. The number of introns varied from one to nine in the ClWRKY gene family. The “WRKYGQK” motif is conserved in 118 members, while other members showed slight variations. AuR and GRE responsive cis-acting elements were located in the promoter region of WRKY members, which are important for plant development and flowering induction. In addition, the W box was present in most genes; the recognition site for the WRKY gene may play a role in autoregulation and cross-regulation. The expression of the most variable 19 genes in terms of different parameters was observed at different stages. Among them, 10 genes were selected due to the presence of CpG islands, while nine genes were selected based on their close association with important Arabidopsis genes related to floral traits. ClWRKY36 and ClWRKY45 exhibit differential expression at flowering stages in the capitulum, while methylation is detected in three genes, including ClWRKY31, ClWRKY100, and ClWRKY129. Our results provide a basis for further exploration of WRKY members to find their functions in plant growth and development, especially in flowering traits.

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Regulation of flowering time in chrysanthemum by the R2R3 MYB transcription factor CmMYB2 is associated with changes in gibberellin metabolism

Cited by 34 publications

References 43 publications

Genetic and Transcriptomic Analysis Reveal the Molecular Basis of Photoperiod-Regulated Flowering in Xishuangbanna Cucumber (Cucumis sativus L. var. xishuangbannesis Qi et Yuan)

Genetic and Transcriptomic Analysis Reveal the Molecular Basis of Photoperiod-Regulated Flowering in Xishuangbanna Cucumber (Cucumis sativus L. var. xishuangbannesis Qi et Yuan)

Overexpression of the CmJAZ1-like gene delays flowering in Chrysanthemum morifolium

Characterization of WRKY Gene Family in Whole-Genome and Exploration of Flowering Improvement Genes in Chrysanthemum lavandulifolium

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