Regulation of filamentation in the human fungal pathogen <scp><i>C</i></scp><i>andida tropicalis</i>

Zhang, Qiuyu; Li, Tao; Guan, Guijian; Yue, Huizhen; Liang, Weihong; Cao, Chengjun; Dai, Yu; Huang, Guanghua

doi:10.1111/mmi.13247

Cited by 38 publications

(62 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a recently published study, approximately 40 transcription factors regulating filamentous growth were identified in C . tropicalis under a series of inducing condition [31]. Transcription regulator, Tec1 , was also found in our study, which demonstrate a conserved role of Tec1 in regulation of filamentation, in consistent with their homologues in C .…”

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

A Genome-Wide Transcriptional Analysis of Yeast-Hyphal Transition in Candida tropicalis by RNA-Seq

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

Candida tropicalis is considered as the leading pathogen in nosocomial fungemia and hepatosplenic fungal infections in patients with cancer, particularly in leukemia. The yeast-filament transition is required for virulent infection by Candida. Several studies have explored the genome-wide transcription profile of Candida, however, no report on the transcriptional profile of C. tropicalis under yeast-filament transition has been published.In this study, the transcriptomes of three C. tropicalis isolates with different adhesion and biofilm formation abilities, identified in our previous studies, were analyzed in both the yeast and filament states using RNA-Seq. Differentially expressed genes were found for each isolate during the transition. A total of 115 genes were up- or down- regulated in the two hyphal-producing isolates (ZRCT 4 and ZRCT 45). Among these differentially expressed genes, only two were down-regulated during the yeast-filament transition. Furthermore, six filament-associated genes were up-regulated in the hyphae-producing isolates. According to Candida Hypha Growth Database established in this study, 331 hyphae- related genes were discovered in C. tropicalis. ALS1 and ALS3 were down-regulated and up-regulated, respectively, during filamentous growth of C. tropicalis. These findings proved a better understanding of gene expression dynamics during the yeast-filament transition in C. tropicalis.

show abstract

Section: Discussionsupporting

confidence: 87%

“…In a previous study, 38 regulators of filamentous growth were identified by screening an overexpression library of 156 transcription factors of C . tropicalis [31], most of which demonstrated a conserved role in the regulation of filamentation, similar to their homolog in C . albicans or Saccharomyces cerevisiae .…”

Section: Resultsmentioning

confidence: 99%

A Genome-Wide Transcriptional Analysis of Yeast-Hyphal Transition in Candida tropicalis by RNA-Seq

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Consistently, we have reported that the Ras/cAMP/PKA pathway plays important roles in filamentous growth and white‐opaque transition, but none of the pathway components is essential for cell viability in C. tropicalis . We successfully generated a tpk1/tpk1 tpk2/tpk2 double mutant in C. tropicalis (Zhang et al ., ; Zheng et al ., ). A more recent study has verified that the PKA catalytic subunit is not required for cell growth in C. tropicalis but is important for virulence (Lin et al ., ).…”

Section: Roles Of the Ras/camp/pka Pathway In The Regulation Of Cell mentioning

confidence: 97%

“…However, the null mutant of CYR1 grows extremely slowly compared to the wild-type strain and ras1/ras1 mutant, implying that the basal activity of Cyr1 required for cell growth is independent of Ras1. We recently found that the deletion of either RAS1 or CYR1 in C. tropicalis, a species closely related to C. albicans, dramatically decreased the growth rate, suggesting that both Ras1 and its effector Cyr1 are critical for cell growth in this species (Zhang et al, 2016). It had long been thought that the catalytic subunit of PKA was essential for cell viability in C. albicans .…”

Section: Roles Of the Ras/camp/pka Pathway In The Regulation Of Cell mentioning

confidence: 99%

Multiple roles and diverse regulation of the Ras/cAMP/protein kinase A pathway in Candida albicans

Huang

Wei

et al. 2018

Molecular Microbiology

Self Cite

View full text Add to dashboard Cite

Summary Candida albicans is a major fungal pathogen of humans, causing both superficial and life‐threatening systemic infections in immunocompromised people. The conserved Ras/cAMP/PKA pathway plays a key role in regulating multiple traits important for the virulence of C. albicans such as cell growth, yeast‐hyphal transition, white‐opaque switching, sexual reproduction and biofilm development. Diverse external signals influence cell physiology by activating this signaling pathway. The key components of the Ras/cAMP/PKA pathway include two Ras GTPases (Ras1 and Ras2), an adenylyl cyclase (Cyr1, also known as Cdc35), two cyclic nucleotide phosphodiesterases (Pde1 and Pde2) and the catalytic (Tpk1 and Tpk2) and regulatory (Bcy1) subunits of PKA kinase. Activation of this pathway dramatically alters the gene expression profile via several transcription factors, leading to the activation of specific biological processes. Here, we review the progress made in the past two decades to elucidate the molecular mechanisms by which the Ras/cAMP/PKA pathway senses diverse environmental cues and controls specific cellular responses and its connection with other signaling pathways in C. albicans.

show abstract

“…For the thermally dimorphic human fungal pathogens, Histoplasma capsulatum and Blastomyces dermatitidis, which grow as yeast at 37 • C in the host and as filamentous hyphae at lower temperatures in the environment, GlcNAc promotes a more rapid return to hyphal growth after a shift to lower temperature [25]. In contrast, GlcNAc has been shown to inhibit hyphal growth in Candida tropicalis, although GlcNAc was still able to induce the White to Opaque switch [102,103], and it was not able to induce true hyphae in Candida dubliniensis [104]. It is also interesting that glucosamine, but not GlcNAc, can stimulate filamentous growth of Cryptococcus neoformans [105].…”

Section: Glcnac Can Stimulate or Inhibit Hyphal Morphogenesis In Othementioning

confidence: 99%

N-Acetylglucosamine Regulates Morphogenesis and Virulence Pathways in Fungi

Min

Naseem

Konopka

2019

JoF

View full text Add to dashboard Cite

N-acetylglucosamine (GlcNAc) is being increasingly recognized for its ability to stimulate cell signaling. This amino sugar is best known as a component of cell wall peptidoglycan in bacteria, cell wall chitin in fungi and parasites, exoskeletons of arthropods, and the extracellular matrix of animal cells. In addition to these structural roles, GlcNAc is now known to stimulate morphological and stress responses in a wide range of organisms. In fungi, the model organisms Saccharomyces cerevisiae and Schizosaccharomyces pombe lack the ability to respond to GlcNAc or catabolize it, so studies with the human pathogen Candida albicans have been providing new insights into the ability of GlcNAc to stimulate cellular responses. GlcNAc potently induces C. albicans to transition from budding to filamentous hyphal growth. It also promotes an epigenetic switch from White to Opaque cells, which differ in morphology, metabolism, and virulence properties. These studies have led to new discoveries, such as the identification of the first eukaryotic GlcNAc transporter. Other results have shown that GlcNAc can induce signaling in C. albicans in two ways. One is to act as a signaling molecule independent of its catabolism, and the other is that its catabolism can cause the alkalinization of the extracellular environment, which provides an additional stimulus to form hyphae. GlcNAc also induces the expression of virulence genes in the C. albicans, indicating it can influence pathogenesis. Therefore, this review will describe the recent advances in understanding the role of GlcNAc signaling pathways in regulating C. albicans morphogenesis and virulence.

show abstract

Regulation of filamentation in the human fungal pathogen Candida tropicalis

Cited by 38 publications

References 89 publications

A Genome-Wide Transcriptional Analysis of Yeast-Hyphal Transition in Candida tropicalis by RNA-Seq

A Genome-Wide Transcriptional Analysis of Yeast-Hyphal Transition in Candida tropicalis by RNA-Seq

Multiple roles and diverse regulation of the Ras/cAMP/protein kinase A pathway in Candida albicans

N-Acetylglucosamine Regulates Morphogenesis and Virulence Pathways in Fungi

Contact Info

Product

Resources

About