Regulation of Fatty Acids Degradation in Bacteria

Jiménez-Díaz, Lorena; Caballero, Antonio; Segura, Ana

doi:10.1007/978-3-319-39782-5_44-1

Cited by 10 publications

(10 citation statements)

References 83 publications

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“…The ratio of dietary fat to starch has been shown to influence the microbiome composition and function in dogs [ 98 ]. Dietary fat that escapes digestion is available in the colon as a nutrient source for resident bacteria [ 99 ], and gut commensals harbor capacity for degradation of fatty acids [ 100 ] via β-oxidation [ 101 ]. Gut microbial β-oxidation converts longer chain fatty acids to SCFA (e.g., butyrate) and medium chain fatty acids (MCFA; e.g., caproate).…”

Section: Discussionmentioning

confidence: 99%

Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat

Jackson¹,

Waldy²,

Jewell³

2020

PLoS ONE

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Dietary digestion-resistant starch (RS) provides health benefits to the host via gut microbiome-mediated metabolism. The degree to which cats manifest beneficial changes in response to RS intake was examined. Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively). Fecal samples collected after 3 and 6 weeks' feeding were assayed for stool firmness score, short-chain fatty acids, ammonia, and changes to the global metabolome and microbiome; fecal immunoglobulin A (IgA) was analyzed at week 6. Few differences were seen in proximate analyses of the foods; stool firmness scores did not differ. In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats. Fecal IgA concentrations were significantly higher at week 6 with HRS food. RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased. Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors. At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities. Microbiome taxa richness and Shannon and Simpson alpha diversity were significantly higher in the HRS group at both weeks. These data show that feline consumption of grain-derived RS produces potentially beneficial shifts in microbiota-mediated metabolism and increases IgA production.

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Section: Discussionmentioning

confidence: 99%

Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat

Jackson¹,

Waldy²,

Jewell³

2020

PLoS ONE

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“…Since stlB is annotated as a FadD homolog, a fatty acyl-CoA synthetase, converting fatty acids to acyl-CoAs for further fatty acid degradation 24 , its influence on the fatty acid degradation profile was studied. Supplementation of TT01 cultures with azido-labelled palmitic acid (C 16 -fatty acid) and application of click-chemistry showed no difference between WT and stlB mutant since both showed similar degradation profiles in the HPLC-MS analysis(Fig.…”

Section: Resultsmentioning

confidence: 99%

Biosynthesis of the Multifunctional Isopropylstilbene inPhotorhabdus laumondiiInvolves Cross-Talk between Specialized and Primary Metabolism

Kavakli

Grammbitter

Bode

2022

Preprint

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Isopropylstilbene (IPS) derived from the entomopathogenic bacteriumPhotorhabdusrepresents the only known stilbene which is not produced by a plant stilbene synthase but a bacterial PKS II synthase. While the exclusive cyclization reaction, responsible for the formation of the characteristic iso-branched side-chain of the molecule, was studied in the past, some parts of the biosynthetic route remained elusive. In this study, we revealed the role of StlB that is able to produce CoA-derivatives and demonstrated the elongation of cinnamoyl-CoA with enzymes from the bacterial fatty acid biosynthesis pathway. Thus, we deciphered cross-talk between the enzymes from primary and specialized metabolism. These insights led, for the first time, to the production of IPS in a heterologous host.

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“… (a) Illustration to show the bacterial FOG catabolism pathway (adapted from [ 92 ]). FadL, long-chain fatty acid transport protein; FadD, fatty acid CoA ligase; FadE, acyl-CoA dehydrogenase; FadA, 2-ketoacyl-CoA thiolase; FadB, enoyl-coA hydratase; LCFA, long-chain fatty acid.…”

Section: Resultsmentioning

confidence: 99%

Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site

et al. 2021

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Sewer systems are complex physical, chemical and microbial ecosystems where fats, oils and grease (FOG) present a major problem for sewer management. Their accumulation can lead to blockages (‘Fatbergs’), sewer overflows and disruption of downstream wastewater treatment. Further advancements of biological FOG treatments need to be tailored to degrade the FOG, and operate successfully within the sewer environment. In this study we developed a pipeline for isolation of lipolytic strains directly from two FOG blockage sites in the UK, and isolated a range of highly lipolytic bacteria. We selected the five most lipolytic strains using Rhodamine B agar plates and pNP-Fatty acid substrates, with two Serratia spp., two Klebsiella spp. and an environmental Acinetobacter strain that all have the capacity to grow on FOG-based carbon sources. Their genome sequences identified the genetic capacity for fatty acid harvesting (lipases), catabolism and utilization (Fad genes). Furthermore, we performed a preliminary molecular characterization of the microbial community at these sites, showing a diverse community of environmental bacteria at each site, but which did include evidence of sequences related to our isolates. This study provides proof of concept to isolation strategies targeting Fatberg sites to yield candidate strains with bioremediation potential for FOG in the wastewater network. Our work sets the foundation for development of novel bioadditions tailored to the environment with non-pathogenic Acinetobacter identified as a candidate for this purpose.

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Regulation of Fatty Acids Degradation in Bacteria

Cited by 10 publications

References 83 publications

Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat

Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat

Biosynthesis of the Multifunctional Isopropylstilbene inPhotorhabdus laumondiiInvolves Cross-Talk between Specialized and Primary Metabolism

Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site

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