Regulation of extracellular protease production in Streptomyces clavuligerus

Abstract

The production of extracellular protease by Streptomyces clavuligerus was strongly influenced by the nature of the nitrogen source. Production took place in batch cultures during growth at low or intermediate growth rates, but was delayed to the post-exponential phase in media supporting high growth rates, Protease formation could be initiated by a nutritional shift-down induced by casamino acids deprivation. Under both types of conditions maximal production was related to the growth rates of the cultures and was stimulated by low concentrations of casamino acids or yeast extract. Some purine compounds also influenced production in shift-down .conditions. A m m o n i u m interfered with protease formation whenever it was added to the medium. Some mutants with altered nitrogen control of primary metabolism were also affected in the production of protease. A partial characterization of the activity indicated that it was due to a single metalloprotease with an apparent molecular mass of 41,700 Da.

Partial Characterization of Proteases From Streptomyces Clavuligerus Using an Inexpensive Medium

“…Both figures show that the activity keeps going up, but the biomass decreases after 50 hours of growth. These results are in agreement with Bascaran et al (1), who showed that synthesis of protease by Streptomyces clavuligerus starts in the post-exponential phase of growth. As we can see from Fig.…”

Purification and Properties of an Extracellular Aminopeptidase from Streptomyces Lividans 1326

“…Taken altogether, the cumulative data indicate that S. lividans probably does not contain serine proteinases, or at least not significant serine proteinase activity, contrary to what was previously indicated (Aretz et al, 1989). Similarly, Streptomyces clavuligerus apparently has a neutral proteinase as its only major extracellular proteinase activity (Bascaran et al, 1990). This is rather unusual, however, since most streptomycetes produce multiple types of proteinases, including serine and metalloproteinase endopeptidases and amino-and carboxypeptidases (Narahashi, 1970 ;Pokorny et al, 1979;Chandrasekaran & Dhar, 1987).…”

Secretion of an alkaline protease from a salt- tolerant and alkaliphilic, Streptomyces clavuligerus strain Mit-1

“…Similarly, Streptomyces pristinaespiralis has also been reported to use alanine and glutamate as the sole source of nitrogen (9). Bascaran et al, (3) stated that better protease production could be obtained with nitrogen-free medium or poorly utilized amino acids.…”