Regulation of expression of the amino acid transporter gene <i>BAP3</i> in <i>Saccharomyces cerevisiae</i>

Boer, M.H. de; Bebelman, Jan Paul; Gonçalves, Paula; Maat, Jaap; Heerikhuizen, Harm van; Planta, Rudi J.

doi:10.1046/j.1365-2958.1998.01094.x

Cited by 48 publications

(54 citation statements)

References 27 publications

(37 reference statements)

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“…This pathway appears to be strikingly simple. Despite extensive genetic screens carried out in several independent laboratories (Ljungdahl et al 1992;Grauslund et al 1995;Didion et al 1996;de Boer et al 1998;Jør-gensen et al 1998;Bernard and André 2001a;Forsberg et al 2001), additional components have not previously been identified. A potential explanation for the lack of additional components being identified is the redundant and overlapping activities of Stp1p and Stp2p.…”

Section: Discussionmentioning

confidence: 99%

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Receptor-mediated endoproteolytic activation of two transcription factors in yeast

Andréasson¹,

Ljungdahl²

2002

Genes Dev.

113

165

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Yeast possess a plasma membrane sensor of external amino acids that functions as a ligand-activated receptor. This multimeric sensor, dubbed the SPS sensor, initiates signals that regulate the expression of genes required for proper amino acid uptake. Stp1p and Stp2p are transcription factors that bind to specific sequences within the promoters of SPS-sensor-regulated genes. These factors exhibit redundant and overlapping abilities to activate transcription. We have found that Stp1p and Stp2p are synthesized as latent cytoplasmic precursors. In response to extracellular amino acids, the SPS sensor induces the rapid endoproteolytic processing of Stp1p and Stp2p. The processing of Stp1p/Stp2p occurs independently of proteasome function and without the apparent involvement of additional components. The shorter forms of these transcription factors, lacking N-terminal inhibitory domains, are targeted to the nucleus, where they transactivate SPS-sensor target genes. These results define a completely unique and streamline metabolic control pathway that directly routes environmental signals initiated at the plasma membrane to transcriptional activation in the nucleus of yeast.

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Section: Discussionmentioning

confidence: 99%

“…In detailed studies focused on the promoters of BAP2 and BAP3, an aminoacid-dependent upstream activating sequence (UAS aa ) was identified (de Boer et al 1998). Both Stp1p and Stp2p are able to bind DNA fragments containing the UAS aa (de Boer et al 2000;Nielsen et al 2001).…”

mentioning

confidence: 99%

Receptor-mediated endoproteolytic activation of two transcription factors in yeast

Andréasson¹,

Ljungdahl²

2002

Genes Dev.

113

165

View full text Add to dashboard Cite

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“…The region bound by the Cup9p repressor has been narrowed to the region spanning À897 to À448 [34], relative to the PTR2 start codon. In addition, a predicted amino acid-dependent upstream activator sequence (UAS AA ) is located between À777 and À760 [69]. Genomic DNA was prepared using the ''smash and grab'' technique [70].…”

Section: Methodsmentioning

confidence: 99%

Harnessing Natural Diversity to Probe Metabolic Pathways

Homann¹,

Cai²,

Becker³

et al. 2005

PLoS Genet

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Analyses of cellular processes in the yeast Saccharomyces cerevisiae rely primarily upon a small number of highly domesticated laboratory strains, leaving the extensive natural genetic diversity of the model organism largely unexplored and unexploited. We asked if this diversity could be used to enrich our understanding of basic biological processes. As a test case, we examined a simple trait: the utilization of di/tripeptides as nitrogen sources. The capacity to import small peptides is likely to be under opposing selective pressures (nutrient utilization versus toxin vulnerability) and may therefore be sculpted by diverse pathways and strategies. Hitherto, dipeptide utilization in S. cerevisiae was solely ascribed to the activity of a single protein, the Ptr2p transporter. Using high-throughput phenotyping and several genetically diverse strains, we identified previously unknown cellular activities that contribute to this trait. We find that the Dal5p allantoate/ureidosuccinate permease is also capable of facilitating di/ tripeptide transport. Moreover, even in the absence of Dal5p and Ptr2p, an additional activity-almost certainly the periplasmic asparaginase II Asp3p-facilitates the utilization of dipeptides with C-terminal asparagine residues by a different strategy. Another, as-yet-unidentified activity enables the utilization of dipeptides with C-terminal arginine residues. The relative contributions of these activities to the utilization of di/tripeptides vary among the strains analyzed, as does the vulnerability of these strains to a toxic dipeptide. Only by sampling the genetic diversity of multiple strains were we able to uncover several previously unrecognized layers of complexity in this metabolic pathway. High-throughput phenotyping facilitates the rapid exploration of the molecular basis of biological complexity, allowing for future detailed investigation of the selective pressures that drive microbial evolution.Citation: Homann OR, Cai H, Becker JM, Lindquist SL (2005) Harnessing natural diversity to probe metabolic pathways. PLoS Genet 1(6): e80.

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“…Upon induction by extracellular amino acids, the SPS sensor catalyzes an endoproteolytic processing event that cleaves the regulatory N-terminal domains. The shorter forms of Stp1 and Stp2 efficiently target to the nucleus where they bind promoters of a limited set of genes, including a subset of broad-specificity amino acid permeases (cluster 1, Table 4) and the peptide transporter Ptr2 (Didion et al 1996(Didion et al , 1998de Boer et al 1998de Boer et al , 2000Iraqui et al 1999;Klasson et al 1999;Wielemans et al 2010;Tumusiime et al 2011).…”

Section: Pathway Genesmentioning

confidence: 99%

Regulation of Amino Acid, Nucleotide, and Phosphate Metabolism in Saccharomyces cerevisiae

2012

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Ever since the beginning of biochemical analysis, yeast has been a pioneering model for studying the regulation of eukaryotic metabolism. During the last three decades, the combination of powerful yeast genetics and genome-wide approaches has led to a more integrated view of metabolic regulation. Multiple layers of regulation, from suprapathway control to individual gene responses, have been discovered. Constitutive and dedicated systems that are critical in sensing of the intra- and extracellular environment have been identified, and there is a growing awareness of their involvement in the highly regulated intracellular compartmentalization of proteins and metabolites. This review focuses on recent developments in the field of amino acid, nucleotide, and phosphate metabolism and provides illustrative examples of how yeast cells combine a variety of mechanisms to achieve coordinated regulation of multiple metabolic pathways. Importantly, common schemes have emerged, which reveal mechanisms conserved among various pathways, such as those involved in metabolite sensing and transcriptional regulation by noncoding RNAs or by metabolic intermediates. Thanks to the remarkable sophistication offered by the yeast experimental system, a picture of the intimate connections between the metabolomic and the transcriptome is becoming clear.

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Regulation of expression of the amino acid transporter gene BAP3 in Saccharomyces cerevisiae

Cited by 48 publications

References 27 publications

Receptor-mediated endoproteolytic activation of two transcription factors in yeast

Receptor-mediated endoproteolytic activation of two transcription factors in yeast

Harnessing Natural Diversity to Probe Metabolic Pathways

Regulation of Amino Acid, Nucleotide, and Phosphate Metabolism in Saccharomyces cerevisiae

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