Regulation of epidermal stratification and development by basal keratinocytes

Yin, Hanxiao; Hu, Mingzheng; Li, Dengwen

doi:10.1002/jcp.30978

Cited by 6 publications

(4 citation statements)

References 55 publications

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“…In spheroids, HaCaT cells were also found to exhibit a stratification with more differentiated cells towards the spheroid rim [20] . Furthermore, during epidermal differentiation, keratinocytes become postmitotic before they go into apoptosis [48] . Thus, as for many epithelial cell types, there is an inverse correlation between proliferation and differentiation of epidermal keratinocytes.…”

Section: Discussionmentioning

confidence: 99%

A Multiparametric Analysis Reveals Differential Behavior of Spheroid Cultures on Distinct Ultra-Low Attachment Plates Types

Vitacolonna,

Bruch,

Agaçi

et al. 2024

Preprint

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Spheroids have become principal three-dimensional biological models to study cancer, developmental processes, and drug efficacy. For spheroid generation, ultra-low attachment plates are noteworthy due to their simplicity, compatibility with automation, and experimental and commercial accessibility. Nonetheless, it is unknown whether and to what degree the plate type impacts spheroid formation and biology. This study employed automated brightfield microscopy to systematically compare the size and eccentricity of spheroids formed in six different plate types using four distinct human cell lines, i.e., CCD-1137Sk fibroblasts, HaCaT keratinocytes, and MDA-MB-231 and HT-29 cancer cells. Results showed that all plate types exhibited similar spheroid-forming capabilities, and the gross patterns of growth or shrinkage during four days after seeding were comparable. Yet, size and eccentricity varied systematically among specific cell lines and plate types. A confocal wholemount analysis by a novel pipeline of AI-based 3D-image analysis procedures revealed changes in cell proliferation, cell number, nuclear volume, and keratinocyte differentiation, which were accompanied by altered YAP1-signals. The findings show that the plate type may influence the outcome of experimental campaigns. It is advisable to scan different plate types for the optimal configuration for a specific investigation instead of using one standard plate for all kinds of applications.

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Section: Discussionmentioning

confidence: 99%

A Multiparametric Analysis Reveals Differential Behavior of Spheroid Cultures on Distinct Ultra-Low Attachment Plates Types

Vitacolonna,

Bruch,

Agaçi

et al. 2024

Preprint

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“…Basal keratinocytes are associated with skin erosion by differential expression. These cells they are known to possess properties of stem cells and are essential in maintaining the integrity of skin and damage recovery (Yin et al, 2023). And respiratory ciliated cells from bronchus are associated with recurrent bronchitis according to co-expression analysis.…”

Section: Literature Validationmentioning

confidence: 99%

Differential expression and co-expression reveal cell types relevant to rare disease phenotypes

Alías-Segura,

Pazos,

Chagoyen

2024

Preprint

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Disease phenotypes, serving as valuable descriptors for delineating the spectrum of human pathologies, play a critical role in understanding disease mechanisms. Integration of these phenotypes with single-cell RNA sequencing (scRNA-seq) data facilitates the elucidation of potential associations between phenotypes and specific cell types underlying them, which sheds light on the underlying physiological processes related to these phenotypes. In this study, we utilized scRNA-seq data to infer potential associations between rare disease phenotypes and cell types. Differential expression and co-expression analyses of genes linked to abnormal phenotypes were employed as metrics to identify the involved cell types. Comparative assessments were made against existing phenotype-cell type associations documented in the literature. Our findings underscore the utility of differential expression and co-expression analyses in identifying significant relationships. Moreover, co-expression analysis unveils cell types potentially linked to abnormal phenotypes not extensively characterized in prior studies.Key points-Cell types underling rare disease phenotypes remain largely unknown-Single-cell RNA-seq data from healthy tissues can be analyzed to reveal these cell types-We employed differential expression and co-expression analysis to identify cell types associated with rare disease phenotypes-We validated our results with known relations described in the literature

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“…The Notch signaling pathway will trigger K1 expression in these cells, and in the mature postnatal spinous layer' keratinocytes (after the intermediate cell layer's dissolution in the embryo's development). Depending on the variation of the Ca 2+ concentrations on both sides of the cytoplasmic membrane, mature spinous keratinocytes will further begin the terminal differentiation process and migrate towards the surface, generating the granular and the cornified layers [7].…”

Section:  Keratinocytesmentioning

confidence: 99%

Untitled

2023

Rom J Morphol Embryol

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The paper provides an overview of the current understanding of different cells' biology (e.g., keratinocytes, Paneth cells, myoepithelial cells, myofibroblasts, chondroclasts, monocytes, atrial cardiomyocytes), including their origin, structure, function, and role in disease pathogenesis, and of the latest findings in the medical literature concerning the brown adipose tissue and the juxtaoral organ of Chievitz.

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Regulation of epidermal stratification and development by basal keratinocytes

Cited by 6 publications

References 55 publications

A Multiparametric Analysis Reveals Differential Behavior of Spheroid Cultures on Distinct Ultra-Low Attachment Plates Types

A Multiparametric Analysis Reveals Differential Behavior of Spheroid Cultures on Distinct Ultra-Low Attachment Plates Types

Differential expression and co-expression reveal cell types relevant to rare disease phenotypes

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