Regulation of early Xenopus development by ErbB signaling

Nie, Shuyi; Chang, Chenbei

doi:10.1002/dvdy.20623

Cited by 17 publications

(21 citation statements)

References 83 publications

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“…Neuregulins signal through the EGF receptor ErbB tyrosine kinase, and this signaling pathway has been shown to contribute to convergent and extension movements during gastrulation. However, a knock-down of ErbB3 or ErbB4 did not inhibit Brachyury expression in the notochord, suggesting that ADAM19 function may act through a different pathway (Nie and Chang, 2006, 2007a,b). On the other hand, we found that ADAM19 knock-down decrease pAKT a known target of the EGF signaling pathway.…”

Section: Discussionmentioning

confidence: 98%

Xenopus ADAM19 is involved in neural, neural crest and muscle development

Neuner

Cousin

McCusker

et al. 2009

Mechanisms of Development

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ADAM19 is a member of the meltrin subfamily of ADAM metalloproteases. In Xenopus, ADAM19 is present as a maternal transcript. Zygotic expression starts during gastrulation and is apparent in the dorsal blastopore lip. ADAM19 expression through neurulation and tailbud formation becomes enriched in dorsal structures such as the neural tube, the notochord and the somites. Using morpholino knock-down, we show that a reduction of ADAM19 protein in gastrula stage embryos results in a decrease of Brachyury expression in the notochord concomitant with an increase in the dorsal markers, Goosecoid and Chordin. These changes in gene expression are accompanied by a decrease in phosphorylated AKT, a downstream target of the EGF signaling pathway, and occur while the blastopore closes at the same rate as the control embryos. During neurulation and tailbud formation, ADAM19 knock-down induces a reduction of the neural markers N-tubulin and NRP1 but not Sox2. In the somitic mesoderm, the expression of MLC is also decreased while MyoD is not. ADAM19 knockdown also reduces neural crest markers prior to cell migration. Neural crest induction is also decreased in embryos treated with an EGF receptor inhibitor suggesting that this pathway is necessary for neural crest cell induction. Using targeted knockdown of ADAM19 we show that the reduction of neural and neural crest markers is cell autonomous and that the migration if the cranial neural crest is perturbed. We further show that ADAM19 protein reduction affects somite organization, reduces 12–101 expression and perturbs fibronectin localization at the intersomitic boundary.

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Section: Discussionmentioning

confidence: 98%

Xenopus ADAM19 is involved in neural, neural crest and muscle development

Neuner

Cousin

McCusker

et al. 2009

Mechanisms of Development

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“…Overexpression in Frog Embryos-Embryos were obtained and maintained as described previously (27). All experiments involving X. laevis were approved by University of Alabama at Birmingham Institutional Animal Care and Use Committee.…”

Section: Methodsmentioning

confidence: 99%

Short Chain Dehydrogenase/Reductase Rdhe2 Is a Novel Retinol Dehydrogenase Essential for Frog Embryonic Development

Belyaeva

Lee

Adams

et al. 2012

Journal of Biological Chemistry

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Background: Retinoic acid regulates expression of numerous genes, but the enzymes required for its biosynthesis are not fully defined. Results: Rdhe2 oxidizes retinol for retinoic acid biosynthesis, and its down-regulation is lethal for frog embryos. Conclusion: Rdhe2 is a previously unrecognized retinol dehydrogenase required for retinoic acid biosynthesis. Significance: Enzymes homologous to frog rdhe2 may carry out similar functions in other species.

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“…The distribution of activated PI3K signaling components during early Xenopus development has not been described. ErbB receptors, as well as the neuregulin 1 (NRG1) ligand, are expressed maternally and throughout early frog embryogenesis (Yang et al, 1998(Yang et al, , 1999Nie and Chang, 2006). Since inhibition of ErbB signaling does not block mesoderm formation and patterning but interferes with gastrulation Chang, 2006, 2007), we propose that ErbB signaling mainly regulates the second phase of PI3K and MAPK activation during gastrulation, but is dispensable for PI3K-and MAPKdependent mesodermal induction.…”

Section: Pi3k and Mapk In Mesoderm Induction And Gastrulationmentioning

confidence: 96%

PI3K and Erk MAPK mediate ErbB signaling in Xenopus gastrulation

Nie

Chang

2007

Mechanisms of Development

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ErbB signaling regulates cell adhesion and movements during Xenopus gastrulation, but the downstream pathways involved have not been elucidated. In this study, we show that phosphatidylinositol-3 kinase (PI3K) and Erk mitogen-activated protein kinase (MAPK) mediate ErbB signaling to regulate gastrulation. Both PI3K and MAPK function sequentially in mesoderm specification and movements, and ErbB signaling is important only for the late phase activation of these pathways to control cell behaviors. Activation of either PI3K or Erk MAPK rescues gastrulation defects in ErbB4 morphant embryos, and restores convergent extension in the trunk mesoderm as well as coherent cell migration in the head mesoderm. The two signals preferentially regulate different aspects of cell behaviors, with PI3K more efficient in rescuing cell adhesion and spreading and MAPK more effective in stimulating the formation of filopodia. PI3K and MAPK also weakly activate each other, and together they modulate gastrulation movements. Our results reveal that PI3K and Erk MAPK, which have previously been considered as mesodermal inducing signals, also act downstream of ErbB signaling to participate in regulation of gastrulation morphogenesis.

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Regulation of early Xenopus development by ErbB signaling

Cited by 17 publications

References 83 publications

Xenopus ADAM19 is involved in neural, neural crest and muscle development

Xenopus ADAM19 is involved in neural, neural crest and muscle development

Short Chain Dehydrogenase/Reductase Rdhe2 Is a Novel Retinol Dehydrogenase Essential for Frog Embryonic Development

PI3K and Erk MAPK mediate ErbB signaling in Xenopus gastrulation

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