Regulation of Drosophila courtship behavior by the Tlx/tailless-like nuclear receptor, dissatisfaction

Duckhorn, Julia C.; Cande, Jessica; Metkus, Mary C.; Song, Hyeop; Altamirano, Sofia; Stern, David L.; Shirangi, Troy R.

doi:10.1016/j.cub.2022.02.031

Cited by 9 publications

(18 citation statements)

References 45 publications

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“…The DDAG neurons are anatomically diverse Drosophila (D.) melanogaster females and males have eleven and three dsf-and dsx-co-expressing abdominal ganglion (DDAG) neurons, respectively, which contribute to several female-and male-specific mating behaviors (Figure 1A) 9 . As a first step toward associating specific courtship functions to specific DDAG neurons, we employed a stochastic labeling method to determine the anatomy of individual DDAG neurons in females and males.…”

Section: Resultsmentioning

confidence: 99%

“…The DDAG neurons are labeled by a genetic intersectional strategy whereby a Flp recombinase, driven in dsx -expressing cells by dsx LexA::p65 10 , excises a transcriptional stop casseae from an upstream activating sequence (UAS)-regulated myr::gfp transgene. Expression of myr::gfp is activated in dsf -co-expressing cells by the dsf Gal4 allele 9 . To stochastically label individual DDAG neurons using a similar intersectional strategy, we utilized FlpSwitch 11 , a Flp recombinase fused to the ligand binding domain of the human Progesterone Receptor (hPR).…”

Section: Resultsmentioning

confidence: 99%

“…Photoactivation of all DDAG neurons induces vaginal plate opening in unmated females and ovipositor extrusion in mated females 9 . Activating the DDAG_B-D neurons, however, induces ovipositor extrusion regardless of mating status (Figure 2D).…”

Section: Discussionmentioning

confidence: 99%

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Developmental remodeling repurposes larval neurons for sexual behaviors in adultDrosophila

Diamandi,

Duckhorn,

Miller

et al. 2023

Preprint

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SummaryMost larval neurons inDrosophilaare repurposed during metamorphosis for functions in adult life, but their contribution to the neural circuits for sexually dimorphic behaviors is unknown. Here, we identify two interneurons in the nerve cord of adultDrosophilafemales that control ovipositor extrusion, a courtship rejection behavior performed by mated females. We show that these two neurons are present in the nerve cord of larvae as mature, sexually monomorphic interneurons. During pupal development, they acquire the expression of the sexual differentiation gene,doublesex, undergodoublesex-dependent programmed cell death in males, and are remodeled in females for functions in female mating behavior. Our results demonstrate that the neural circuits for courtship inDrosophilaare built in part using neurons that are sexually reprogrammed from former sex-shared activities in larval life.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Developmental remodeling repurposes larval neurons for sexual behaviors in adultDrosophila

Diamandi,

Duckhorn,

Miller

et al. 2023

Preprint

Self Cite

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“…From these findings emerges the concept that a delicate tuning exists between sex‐specific behaviours and experience, partly executed by the role of fru/dsx‐ expressing neurons. In line with this, studies in abdominal courtship behaviours of the fly have revealed sex‐specific circuit changes dictated by a combination of the transcription factors dsx and dsf [85]. One question remains: Could environmental cues alter synaptic/circuit states independent of the sex‐determination pathway to trigger sex‐specific responses?…”

Section: Experience‐dependent Sexual Dimorphic Behaviours In Drosophi...mentioning

confidence: 98%

Environmental experiences shape sexually dimorphic neuronal circuits and behaviour

2023

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Dimorphic traits, shaped by both natural and sexual selection, ensure optimal fitness and survival of the organism. This includes neuronal circuits that are largely affected by different experiences and environmental conditions. Recent evidence suggests that sexual dimorphism of neuronal circuits extends to different levels such as neuronal activity, connectivity and molecular topography that manifest in response to various experiences, including chemical exposures, starvation and stress. In this review, we propose some common principles that govern experience‐dependent sexually dimorphic circuits in both vertebrate and invertebrate organisms. While sexually dimorphic neuronal circuits are predetermined, they have to maintain a certain level of fluidity to be adaptive to different experiences. The first layer of dimorphism is at the level of the neuronal circuit, which appears to be dictated by sex‐biased transcription factors. This could subsequently lead to differences in the second layer of regulation namely connectivity and synaptic properties. The third regulator of experience‐dependent responses is the receptor level, where dimorphic expression patterns determine the primary sensory encoding. We also highlight missing pieces in this field and propose future directions that can shed light onto novel aspects of sexual dimorphism with potential benefits to sex‐specific therapeutic approaches. Thus, sexual identity and experience simultaneously determine behaviours that ultimately result in the maximal survival success.

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“…In situ hybridization chain reaction (HCR) HCR was carried out as previously described in (Duckhorn et al, 2022). 20 pairs of probes were designed, tiled along the eya transcripts and excluding regions of high similarity with other genes, with initiator sequences corresponding to amplifier B3 for amplification (Choi et al, 2018).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Niche signalling regulates eIF3d1 phosphorylation to promote distinct modes of translation initiation in stem and differentiating cells

Wang,

Roiuk,

Storer

et al. 2023

Preprint

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Stem cells have the unique ability among adult cells to give rise to cells of different identities. To do so, they must change gene expression in response to environmental signals. Much work has focused on how transcription is regulated to achieve these changes, however in many cell types, transcripts and proteins correlate poorly, indicating that post-transcriptional regulation is important. To assess how translational control can influence stem cell fate, we use the Drosophila testis as a model. The testis niche secretes a ligand to activate the JAK/STAT pathway in two stem cell populations, germline stem cells (GSCs) and somatic cyst stem cells (CySCs). We find that global translation rates are high in CySCs and decrease during differentiation, and that JAK/STAT signalling regulates translation. To determine how translation was regulated, we knocked down translation initiation factors and found that the cap binding complex, eIF4F, is dispensable in differentiating cells, but is specifically required in CySCs for self-renewal, acting downstream of JAK/STAT activity. Moreover, we identify eIF3d1 as a key regulator of CySC fate, and show that its phosphorylation is critical to maintain CySC self-renewal. We further show that Casein Kinase II, which controls eIF3d1 phosphorylation, is sufficient to restore CySC function in the absence of JAK/STAT. We propose a model in which niche signals regulate a specific translation programme in which only some mRNAs are translated, through regulation of eIF3d phosphorylation. The mechanism we identify allows stem cells to switch between modes of translation, adding a layer of regulation on top of transcription and providing cells with the ability to rapidly change gene expression upon receiving external stimuli.

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Regulation of Drosophila courtship behavior by the Tlx/tailless-like nuclear receptor, dissatisfaction

Cited by 9 publications

References 45 publications

Developmental remodeling repurposes larval neurons for sexual behaviors in adultDrosophila

Developmental remodeling repurposes larval neurons for sexual behaviors in adultDrosophila

Environmental experiences shape sexually dimorphic neuronal circuits and behaviour

Niche signalling regulates eIF3d1 phosphorylation to promote distinct modes of translation initiation in stem and differentiating cells

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