Regulation of cytosolic calcium levels in vascular smooth muscle

Orallo, Francisco

doi:10.1016/0163-7258(95)02042-x

Cited by 80 publications

(94 citation statements)

References 234 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thereafter, the process includes the diffusion of NO into the smooth muscle cells of the media and the stimulation of the soluble (cytosolic) guanylate cyclase, leading to a higher ac- molpharm.aspetjournals.org cumulation of cGMP, which could relax vascular smooth muscle via different mechanisms (Kuriyama et al, 1995;Orallo, 1996). Therefore, to provide new data to clarify this enhancement of the L-arginine-NO-cGMP pathway by t-RESV, different series of experiments were designed.…”

Section: Discussionmentioning

confidence: 99%

The Possible Implication oftrans-Resveratrol in the Cardioprotective Effects of Long-Term Moderate Wine Consumption

Orallo¹,

Álvarez²,

Camiña³

et al. 2002

Mol Pharmacol

Self Cite

235

156

View full text Add to dashboard Cite

trans-Resveratrol (t-RESV; 1-10 M), a phenolic component of wines, had no effect on phenylephrine-(PE; 1 M) and high KCl-(60 mM) induced contractions in endothelium-denuded rat aortic rings. However, it relaxed the contractile response produced by these vasoconstrictor agents in intact rat aorta. The vasorelaxing effects of t-RESV were completely inhibited by N G -nitro-L-arginine (L-NOARG; 0.1 mM) and methylene blue (10 M), but they were unaffected by atropine (10 M) and yohimbine (1 M). The reversal effect produced by L-NOARG was antagonized by L-arginine but not by D-arginine (0.1 mM). t-RESV (1-10 M) did not significantly modify rat aorta constitutive nitric-oxide synthase activity. However, this natural compound decreased NADH/NADPH oxidase activity in rat aortic homogenates. In addition, t-RESV (1-10 M) was ineffective in scavenging superoxide anions (O 2 . ) generated enzymatically by a hypoxanthine/xanthine oxidase (HX/XO) system and/or to inhibit XO. The above data demonstrate that the characteristic endothelium-dependent vasorelaxant effect of t-RESV in rat aorta seems to be caused by the inhibition of vascular NADH/ NADPH oxidase and the subsequent decrease of basal cellular O 2 . generation and, therefore, of NO biotransformation. Under the assumption that t-RESV exhibits a similar behavior in human blood vessels and bearing in mind that an overactivity of NADH/NADPH oxidase has been found in a number of cardiovascular pathologies, the results obtained in this work suggest that t-RESV could play an important role in the cardioprotective effects induced by the long-term moderate wine consumption.

show abstract

Section: Discussionmentioning

confidence: 99%

The Possible Implication oftrans-Resveratrol in the Cardioprotective Effects of Long-Term Moderate Wine Consumption

Orallo¹,

Álvarez²,

Camiña³

et al. 2002

Mol Pharmacol

Self Cite

235

156

View full text Add to dashboard Cite

show abstract

“…Calcium maintains the vitality of organs by contraction and relaxation in smooth muscles and endothelial tissues [Orallo, 1996]. Fluctuations in the intracellular free calcium ion concentration control a number of diverse cellular processes (vasa vasorum flow, endothelial contraction, collagen, elastins, proteoglycans synthesis and platelet activity) affecting various proteases and calcium dependent phospholipase enzymes [Henry, 1990].…”

Section: Iso Mediated Calcium Overloadmentioning

confidence: 99%

Exploration of pharmacological interventions to prevent isoproterenol-induced myocardial infarction in experimental models

Garg

Kumari

2014

Therapeutic Advances in Cardiovascular Disease

View full text Add to dashboard Cite

High incidences of myocardial infarction associated with high morbidity and mortality, are a major concern and economic burden on industrialized nations. Persistent β-adrenergic receptor stimulation with isoproterenol leads to the development of oxidative stress, myocardial inflammation, thrombosis, platelet aggregation and calcium overload, which ultimately cause myocardial infarction. Therapeutic agents that are presently employed for the prevention and management of myocardial infarction are beta-blockers, antithrombotics, thrombolytics, statins, angiotensin converting enzyme inhibitors, angiotensin II type 1 receptor blockers, calcium channel blockers and nitrovasodilators. In spite of effective available interventions, the mortality rate of myocardial infarction is progressively increasing. Thus, there has been a regular need to develop effective therapies for the prevention and management of this insidious disease. In this review, the authors give an overview of the consequences of isoproterenol in the pathogenesis of cardiac disorders and various therapeutic possibilities to prevent these disorders.

show abstract

“…Furthermore, many authors related the effect of EGCG to the inhibition of Ca 2＋ influx in cellular level. For example, EGCG has vasorelaxant effect on vascular smooth muscle cells, possibly by phosphorylation of transmembrane Ca 2＋ channels or associated regulatory proteins (Orlov et al, 1996;Minowa et al, 1997) and inhibits the release of Ca 2＋ from intracellular stores (Orallo, 1996). In the present study, we examined spontaneous [Ca 2＋ ]i oscillations in ICC and found that EGCG inhibited the [Ca 2＋ ]i oscillations.…”

Section: ＋mentioning

confidence: 98%

(-)-Epigallocatechin Gallate Inhibits the Pacemaker Activity of Interstitial Cells of Cajal of Mouse Small Intestine

Kim

Choi

Jang

et al. 2008

Korean J Physiol Pharmacol

View full text Add to dashboard Cite

Regulation of cytosolic calcium levels in vascular smooth muscle

Cited by 80 publications

References 234 publications

The Possible Implication oftrans-Resveratrol in the Cardioprotective Effects of Long-Term Moderate Wine Consumption

The Possible Implication oftrans-Resveratrol in the Cardioprotective Effects of Long-Term Moderate Wine Consumption

Exploration of pharmacological interventions to prevent isoproterenol-induced myocardial infarction in experimental models

(-)-Epigallocatechin Gallate Inhibits the Pacemaker Activity of Interstitial Cells of Cajal of Mouse Small Intestine

Contact Info

Product

Resources

About