Regulation of cuticle formation during fruit development and ripening in ‘Newhall’ navel orange ( Citrus sinensis Osbeck) revealed by transcriptomic and metabolomic profiling

Wang, Jinqiu; Sun, Li; Xie, Li; He, Yuan; Luo, Tao; Sheng, Lianxi; Luo, Yi; Zeng, Yunliu; Xu, Juan; Deng, Xiuxin; Cheng, Yunjiang

doi:10.1016/j.plantsci.2015.12.010

Cited by 90 publications

(95 citation statements)

References 63 publications

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“…for the same mango cultivar. A similar result was reported for orange fruit ( Citrus sinensis Osbeck), where the weight of cuticle per unit increased by almost two-fold at 150 DAF compared with 120 DAF18. It has also been reported that apple fruit exhibit an increase in cuticular wax deposition during ripening, coincident with a burst in ethylene production19.…”

Section: Resultssupporting

confidence: 78%

Transcriptome Analysis of Mango (Mangifera indica L.) Fruit Epidermal Peel to Identify Putative Cuticle-Associated Genes

Tafolla-Arellano

Zheng

Sun

et al. 2017

Sci Rep

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Mango fruit (Mangifera indica L.) are highly perishable and have a limited shelf life, due to postharvest desiccation and senescence, which limits their global distribution. Recent studies of tomato fruit suggest that these traits are influenced by the expression of genes that are associated with cuticle metabolism. However, studies of these phenomena in mango fruit are limited by the lack of genome-scale data. In order to gain insight into the mango cuticle biogenesis and identify putative cuticle-associated genes, we analyzed the transcriptomes of peels from ripe and overripe mango fruit using RNA-Seq. Approximately 400 million reads were generated and de novo assembled into 107,744 unigenes, with a mean length of 1,717 bp and with this information an online Mango RNA-Seq Database (http://bioinfo.bti.cornell.edu/cgi-bin/mango/index.cgi) which is a valuable genomic resource for molecular research into the biology of mango fruit was created. RNA-Seq analysis suggested that the pathway leading to biosynthesis of the cuticle component, cutin, is up-regulated during overripening. This data was supported by analysis of the expression of several putative cuticle-associated genes and by gravimetric and microscopic studies of cuticle deposition, revealing a complex continuous pattern of cuticle deposition during fruit development and involving substantial accumulation during ripening/overripening.

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Section: Resultssupporting

confidence: 78%

Transcriptome Analysis of Mango (Mangifera indica L.) Fruit Epidermal Peel to Identify Putative Cuticle-Associated Genes

Tafolla-Arellano

Zheng

Sun

et al. 2017

Sci Rep

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“…As a crucial hormone in the ripening process, ABA significantly affects carotenoid metabolism in tomato, mango, watermelon, and citrus fruits (Zhang et al ., , ; Zaharah et al ., ; Barickman et al ., ; Wang et al ., ,b). Several important genes involved in carotenoid metabolism, such as PSY3 from maize and DSM2 , a BCH gene from rice, were significantly induced by ABA treatments (Li et al ., ; Du et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…Abscisic acit was extracted from the citrus flavedo from the fruits harvested in 2011 as recommended by Wang et al . (,b). Briefly, 0.2 g of ground sample was added to 750 μl of extraction buffer containing methanol : H 2 O : acetic acid (80 : 19 : 1, v/v/v) in a 2 ml tube.…”

Section: Methodsmentioning

confidence: 99%

An R2R3‐MYB transcription factor represses the transformation of α‐ and β‐branch carotenoids by negatively regulating expression of CrBCH2 and CrNCED5 in flavedo of Citrus reticulate

et al. 2017

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Although the functions of carotenogenic genes are well documented, little is known about the mechanisms that regulate their expression, especially those genes involved in α - and β-branch carotenoid metabolism. In this study, an R2R3-MYB transcriptional factor (CrMYB68) that directly regulates the transformation of α- and β-branch carotenoids was identified using Green Ougan (MT), a stay-green mutant of Citrus reticulata cv Suavissima. A comprehensive analysis of developing and harvested fruits indicated that reduced expression of β-carotene hydroxylases 2 (CrBCH2) and 9-cis-epoxycarotenoid dioxygenase 5 (CrNCED5) was responsible for the delay in the transformation of α- and β-carotene and the biosynthesis of ABA. Additionally, the expression of these genes was negatively correlated with the expression of CrMYB68 in MT. Further, electrophoretic mobility shift assays (EMSAs) and dual luciferase assays indicated that CrMYB68 can directly and negatively regulate CrBCH2 and CrNCED5. Moreover, transient overexpression experiments using leaves of Nicotiana benthamiana indicated that CrMYB68 can also negatively regulate NbBCH2 and NbNCED5. To overcome the difficulty of transgenic validation, we quantified the concentrations of carotenoids and ABA, and gene expression in a revertant of MT. The results of these experiments provide more evidence that CrMYB68 is an important regulator of carotenoid metabolism.

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“…The Arabidopsis flu mutant was described to produce 1 O 2 in leaves when illuminated after a period of darkness because of an overproduction of protochlorophyllide in the dark (op de Camp et al, 2003). Wagner et al (2004) found that EX1 was responsible for programmed cell death in bleached seedlings and growth inhibition in Arabidopsis flu mutants, with the EX1 protein located at the grana margins of chloroplasts mediating responses to 1 O 2 production (Wang et al, 2016). Moreover, a recent study performed in the Arabidopsis mutant chlorina1 showed that programmed cell death induced by 1 O 2 under high levels of light was mediated by OXI1 via an EX1-independent pathway (Shumbe et al, 2016).…”

Section: Redox Signaling During Leaf Flower and Fruit Developmentmentioning

confidence: 99%

Photo-Oxidative Stress during Leaf, Flower and Fruit Development

Muñoz

Munné‐Bosch

2017

Plant Physiol.

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Regulation of cuticle formation during fruit development and ripening in ‘Newhall’ navel orange ( Citrus sinensis Osbeck) revealed by transcriptomic and metabolomic profiling

Cited by 90 publications

References 63 publications

Transcriptome Analysis of Mango (Mangifera indica L.) Fruit Epidermal Peel to Identify Putative Cuticle-Associated Genes

Transcriptome Analysis of Mango (Mangifera indica L.) Fruit Epidermal Peel to Identify Putative Cuticle-Associated Genes

An R2R3‐MYB transcription factor represses the transformation of α‐ and β‐branch carotenoids by negatively regulating expression of CrBCH2 and CrNCED5 in flavedo of Citrus reticulate

Photo-Oxidative Stress during Leaf, Flower and Fruit Development

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