Abstract:Background:We identified target transcripts of the RNA-binding protein CUGBP1 in resting and activated T cells. Results: T cell activation induced CUGBP1 phosphorylation, causing decreased CUGBP1 binding to target transcripts. Conclusion: CUGBP1 binding to a network of target transcripts is regulated by CUGBP1 phosphorylation following T cell activation. Significance: CUGBP1 target transcripts are coordinately regulated during T cell activation.
“…During the process of T cell activation, phosphorylation of CELF1 alters binding by CELF1 to target transcripts. Shortly following T cell activation, CELF1 becomes phosphorylated, dramatically decreasing its affinity for mRNA and leading to stabilization of CELF1 target transcripts [77]. Overall, these studies show that phosphorylation regulates the many functions of CELF1 in posttranscriptional gene regulation.…”
Section: Regulation Of Celf1 Function Through Phosphorylationmentioning
confidence: 73%
“…In primary human T cells, GREs and CELF1 appear to be involved in the rapid changes in gene expression patterns observed following T cell receptor-mediated activation. Identification the cytoplasmic binding targets of CELF1 before and after T cell activation led to the discovery that CELF1 dissociated from GREcontaining transcripts following T cell activation in a manner correlated with a transient upregulation of CELF1 target mRNAs [77]. The dissociation of CELF1 from its target transcripts upon T cell activation was the result of an activation-dependent phosphorylation of CELF1 and a resultant decrease in the ability of CELF1 to bind to GRE-containing RNAs [77].…”
Section: Mrna Decaymentioning
confidence: 99%
“…In this work, the authors proposed a 15-nucleotide consensus motif (UGU/UG)3 to be the target motif of EDEN-BP [85], [86]. The RIP-Chip approach was also used to investigate the cytoplasmic target transcripts of CELF1 in resting and activated primary human T cells, and target transcripts were highly enriched for the presence of the GRE in their 3'UTRs, but the number of CELF1 target transcripts decreased dramatically following T cell activation [77]. Overall, numerous CELF1 target transcripts have been identified in several different systems indicating the CELF1 functions to regulate an important posttranscriptional network of gene expression.…”
Section: Identification Of Celf1 Target Transcriptsmentioning
confidence: 99%
“…Using this methodology, CELF1 targets have been identified in HeLa cells, resting and activated human T cells, and mouse myoblasts [55], [77], [82].…”
Section: Identification Of Celf1 Target Transcriptsmentioning
confidence: 99%
“…CELF1 is a known phosphoprotein with multiple predicted phosphorylation sites, and CELF1 phosphorylation appears to regulate its function as a mediator of alternative splicing, mRNA decay, and translational regulation [74], [75], [76], [77]. One of the pathologic events which occurs in the disease Myotonic Dystrophy type 1 (DM1) is an increase in the protein abundance of CELF1 and an associated increase in CELF1 mediated alternative splicing activity.…”
Section: Regulation Of Celf1 Function Through Phosphorylationmentioning
“…During the process of T cell activation, phosphorylation of CELF1 alters binding by CELF1 to target transcripts. Shortly following T cell activation, CELF1 becomes phosphorylated, dramatically decreasing its affinity for mRNA and leading to stabilization of CELF1 target transcripts [77]. Overall, these studies show that phosphorylation regulates the many functions of CELF1 in posttranscriptional gene regulation.…”
Section: Regulation Of Celf1 Function Through Phosphorylationmentioning
confidence: 73%
“…In primary human T cells, GREs and CELF1 appear to be involved in the rapid changes in gene expression patterns observed following T cell receptor-mediated activation. Identification the cytoplasmic binding targets of CELF1 before and after T cell activation led to the discovery that CELF1 dissociated from GREcontaining transcripts following T cell activation in a manner correlated with a transient upregulation of CELF1 target mRNAs [77]. The dissociation of CELF1 from its target transcripts upon T cell activation was the result of an activation-dependent phosphorylation of CELF1 and a resultant decrease in the ability of CELF1 to bind to GRE-containing RNAs [77].…”
Section: Mrna Decaymentioning
confidence: 99%
“…In this work, the authors proposed a 15-nucleotide consensus motif (UGU/UG)3 to be the target motif of EDEN-BP [85], [86]. The RIP-Chip approach was also used to investigate the cytoplasmic target transcripts of CELF1 in resting and activated primary human T cells, and target transcripts were highly enriched for the presence of the GRE in their 3'UTRs, but the number of CELF1 target transcripts decreased dramatically following T cell activation [77]. Overall, numerous CELF1 target transcripts have been identified in several different systems indicating the CELF1 functions to regulate an important posttranscriptional network of gene expression.…”
Section: Identification Of Celf1 Target Transcriptsmentioning
confidence: 99%
“…Using this methodology, CELF1 targets have been identified in HeLa cells, resting and activated human T cells, and mouse myoblasts [55], [77], [82].…”
Section: Identification Of Celf1 Target Transcriptsmentioning
confidence: 99%
“…CELF1 is a known phosphoprotein with multiple predicted phosphorylation sites, and CELF1 phosphorylation appears to regulate its function as a mediator of alternative splicing, mRNA decay, and translational regulation [74], [75], [76], [77]. One of the pathologic events which occurs in the disease Myotonic Dystrophy type 1 (DM1) is an increase in the protein abundance of CELF1 and an associated increase in CELF1 mediated alternative splicing activity.…”
Section: Regulation Of Celf1 Function Through Phosphorylationmentioning
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