Regulation of Cre Recombinase Activity by Mutated Estrogen Receptor Ligand-Binding Domains

Feil, Robert; Wagner, Jürgen; Metzger, Daniel; Chambon, Pierre

doi:10.1006/bbrc.1997.7124

Cited by 898 publications

(863 citation statements)

References 29 publications

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“…(CreER T2 ), which has been shown to be between 4-and 10-fold more sensitive to 4-hydroxytamoxifen in vivo (Feil et al, 1997;Indra et al, 1999). 4-hydroxytamoxifen is a metabolite of tamoxifen that is generated in the liver and believed to be the active compound in binding the ER, although administration of either compound, at the same concentration, results in indistinguishable recombination efficiency (Kuhbandner et al, 2000).…”

Section: Figmentioning

confidence: 99%

Genetic inducible fate mapping in mouse: Establishing genetic lineages and defining genetic neuroanatomy in the nervous system

Joyner

Zervas

2006

Developmental Dynamics

172

167

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A fascinating aspect of developmental biology is how organs are assembled in three dimensions over time. Fundamental to understanding organogenesis is the ability to determine when and where specific cell types are generated, the lineage of each cell, and how cells move to reside in their final position. Numerous methods have been developed to mark and follow the fate of cells in various model organisms used by developmental biologists, but most are not readily applicable to mouse embryos in utero because they involve physical marking of cells through injection of tracers. The advent of sophisticated transgenic and gene targeting techniques, combined with the use of site-specific recombinases, has revolutionized fate mapping studies in mouse. Furthermore, using genetic fate mapping to mark cells has opened up the possibility of addressing fundamental questions that cannot be studied with traditional methods of fate mapping in other organisms. Specifically, genetic fate mapping allows both the relationship between embryonic gene expression and cell fate (genetic lineage) to be determined, as well as the link between gene expression domains and anatomy (genetic anatomy) to be established. In this review, we present the ever-evolving development of genetic fate mapping techniques in mouse, especially the recent advance of Genetic Inducible Fate Mapping. We then review recent studies in the nervous system (focusing on the anterior hindbrain) as well as in the limb and with adult stem cells to highlight fundamental developmental processes that can be discovered using genetic fate mapping approaches. We end with a look toward the future at a powerful new approach that combines genetic fate mapping with cellular phenotyping alleles to study cell morphology, physiology, and function using examples from the nervous system. Developmental Dynamics 235:2376 -2385, 2006.

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Section: Figmentioning

confidence: 99%

Genetic inducible fate mapping in mouse: Establishing genetic lineages and defining genetic neuroanatomy in the nervous system

Joyner

Zervas

2006

Developmental Dynamics

172

167

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“…We used three different HBD-Cre systems in an effort to develop astrocyte-specific, inducible gene recombination. Two of these are based on a mutated estrogen receptor, CreER T2 and MerCreMer (Feil et al, 1997;Zhang et al, 1996), and the third on a mutated progesterone receptor, CrePR2 (Kellendonk et al, 1996) (see Fig. 1b).…”

Section: The Steroid Responsive Systemsmentioning

confidence: 99%

Characterization of astrocyte‐specific conditional knockouts

Casper

Jones

McCarthy

2007

Genesis

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Summary: Conditional gene knockouts are a very powerful tool for elucidating gene function in animal physiology and behavior. To obtain cell-specific knockouts, a promoter is utilized that drives expression of Cre recombinase specifically to the cell population of interest. We describe several transgenic lines of mice that were created in an attempt to obtain astrocyte-specific gene recombination. A 2 kb fragment from the human glial fibrillary acidic protein promoter is utilized to drive expression of inducible Cre recombinase, with both the Tet-Off and tamoxifen responsive systems. We show data obtained from crosses with two Cre reporter lines, ROSA26R and an astrocyte Cre reporter created in our laboratory, to assess the cell specificity of gene recombination. Additionally, our system is shown to successfully recombine a floxed Connexin43 locus, although recombination is not as extensive as seen in crosses with reporter lines. genesis 45:292-299, 2007. V V C 2007 Wiley-Liss, Inc.

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“…Harfe et al (2004) furthered their analysis using a CreERT2 gene inserted into the Shh locus. CreErt2 encodes a fusion protein between the Cre recombinase and the activation domain of the estrogen nuclear receptor that is activated in embryos when tamoxifen is provided to the mother (Feil et al, 1997). This way, could label Shh-expressing cells at selected times during development.…”

Section: Mechanism Of Action Of Shh: Cellular Aspectsmentioning

confidence: 99%

Anteroposterior patterning in the limb and digit specification: Contribution of mouse genetics

Robert

Lallemand

2006

Developmental Dynamics

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The limb has been a privileged object of investigation and reflection for scientists over the past two centuries and continues to provide a heuristic framework to analyze vertebrate development. Recently, accumulation of new data has significantly changed our view on the mechanisms of limb patterning, in particular along the anterior-posterior axis. These data have led us to revisit the mode of action of the zone of polarizing activity. They shed light on the molecular and cellular mechanisms of patterning linked to the Shh-Gli3 signaling pathway and give insights into the mechanism of activation of these cardinal factors, as well as the consequences of their activity. These new data are in good part the result of systematic Application of tools used in contemporary mouse molecular genetics. These have extended the power of mouse genetics by introducing mutational strategies that allow fine-tuned modulation of gene expression, interchromosomal deletions and duplication. They have even made the mouse embryo amenable to cell lineage analysis that used to be the realm of chick embryos. In this review, we focus on the data acquired over the last five years from the analysis of mouse limb development and discuss new perspectives opened by these results. Developmental Dynamics 235:2337-2352, 2006.

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Regulation of Cre Recombinase Activity by Mutated Estrogen Receptor Ligand-Binding Domains

Cited by 898 publications

References 29 publications

Genetic inducible fate mapping in mouse: Establishing genetic lineages and defining genetic neuroanatomy in the nervous system

Genetic inducible fate mapping in mouse: Establishing genetic lineages and defining genetic neuroanatomy in the nervous system

Characterization of astrocyte‐specific conditional knockouts

Anteroposterior patterning in the limb and digit specification: Contribution of mouse genetics

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