Regulation of corticosterone function during early weaning and effects on gastric cell proliferation

Ghizoni, Heloisa; Figueiredo, Priscila Moreira; Moisan, Marie‐Pierre; Ogias, Daniela; Osaki, Luciana H.; Gama, Patrı́cia

doi:10.1016/j.nut.2013.09.003

Cited by 16 publications

(41 citation statements)

References 48 publications

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“…[61][62][63][64][65] This model involves maternal milk restriction and maternal care restriction, which promotes perinatal stress. 66 Therefore, the offspring outcomes could be adaptive responses to nutritional changes as well as emotional stress.…”

Section: Maternal Deprivationmentioning

confidence: 99%

Does early weaning shape future endocrine and metabolic disorders? Lessons from animal models

Souza

Moura

Lisboa

2020

J Dev Orig Health Dis

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Obesity and its complications occur at alarming rates worldwide. Epidemiological data have associated perinatal conditions, such as malnutrition, with the development of some disorders, such as obesity, dyslipidemia, diabetes, and cardiovascular diseases, in childhood and adulthood. Exclusive breastfeeding has been associated with protection against long-term chronic diseases. However, in humans, the interruption of breastfeeding before the recommended period of 6 months is a common practice and can increase the risk of several metabolic disturbances. Nutritional and environmental changes within a critical window of development, such as pregnancy and breastfeeding, can induce permanent changes in metabolism through epigenetic mechanisms, leading to diseases later in life via a phenomenon known as programming or developmental plasticity. However, little is known regarding the underlying mechanisms by which precocious weaning can result in adipose tissue dysfunction and endocrine profile alterations. Here, the authors give a comprehensive report of the different animal models of early weaning and programming that can result in the development of metabolic syndrome. In rats, for example, pharmacological and nonpharmacological early weaning models are associated with the development of overweight and visceral fat accumulation, leptin and insulin resistance, and neuroendocrine and hepatic changes in adult progeny. Sex-related differences seem to influence this phenotype. Therefore, precocious weaning seems to be obesogenic for offspring. A better understanding of this condition seems essential to reducing the risk for diseases. Additionally, this knowledge can generate new insights into therapeutic strategies for obesity management, improving health outcomes.

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Section: Maternal Deprivationmentioning

confidence: 99%

Does early weaning shape future endocrine and metabolic disorders? Lessons from animal models

Souza

Moura

Lisboa

2020

J Dev Orig Health Dis

View full text Add to dashboard Cite

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“…Previamente, nosso grupo demonstrou que em filhotes as células proliferativas são detectadas ao longo de toda a extensão da glândula, indicando que na fase de crescimento, antes do estabelecimento das regiões glandulares, a proliferação celular não está restrita ao nicho principal (istmo -colo) (Alvares e Gama, 1993;Ghizoni et al, 2014;Zonta et al, 2018). Apesar da diversidade de células epiteliais na glândula gástrica, no presente trabalho destacaremos as populações de células parietais, células mucosas do colo, células zimogênicas e a subpopulação de células em transição.…”

Section: Células-tronco E Progenitorasunclassified

“…Nosso grupo já demonstrou que o DP altera a atividade da corticosterona na mucosa gástrica, elevando os níveis hormonais nos primeiros dias de dieta sólida (Ghizoni et al, 2014), além de provocar uma perda de massa corpórea em filhotes (Gama e Alvares, 2000;Zulian et al, 2017). Outros estudos demonstraram o aumento de populações de CMC e CZ, e de células endócrinas secretoras de ghrelina em filhotes (Osaki et al, 2010;Bittar et al, 2016); Zulian et al 2017), além de alterações da proliferação celular e a espessura da glândula gástrica (Gama e Alves, 2000;Zonta, 2017).…”

Section: Amamentação E O Desmame Precoceunclassified

“…Outros estudos demonstraram o aumento de populações de CMC e CZ, e de células endócrinas secretoras de ghrelina em filhotes (Osaki et al, 2010;Bittar et al, 2016); Zulian et al 2017), além de alterações da proliferação celular e a espessura da glândula gástrica (Gama e Alves, 2000;Zonta, 2017). Essas modificações ocorrem por meio mecanismos de regulação que envolvem a via de sinalização por MAPK (Alvares e Gama, 1993;Osaki et al, 2011;Osaki e Gama, 2013) e receptores de glicocorticóides (Ghizoni et al, 2014).…”

Section: Amamentação E O Desmame Precoceunclassified

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O desmame precoce aumenta e reprograma a diferenciação de células mucosas do colo em células zimogênicas na mucosa gástrica de ratos.

Silva¹

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“…Em linhagens celulares de câncer de próstata, o tratamento com dexametasona também eleva a expressão de Sgk1 e a adição concomitante de RU486 anula a ação do glicocorticoide, entretanto nenhum efeito foi observado nos níveis de Nr3c1 (gene codificante de GR) 120 , dado que foi corroborado por nossas análises. Nossos resultados sugeriram que a administração de RU486 agiu sobre a expressão de Sgk1 na condição DP em que a atividade da corticosterona estava aumentada(Ghizoni et al, 2014), e assim, podemos considerar que a eficiência do antagonista foi maior no desmame precoce em comparação à amamentação.Em relação ao papel da corticostserona durante o desmame precoce, verificamos que o RU486 reverteu o efeito do DP sobre Muc5ac e Pgc em ratos de 17 dias, indicando a relação primordial do hormônio nesses genes. Entretanto, houve aumento de Muc6 nos filhotes amamentados.…”

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Diferenciação celular no epitélio gástrico de ratos submetidos ao desmame precoce: avaliação da ação da corticosterona.

Zulian¹

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The epithelium of the stomach corpus is lined by long tubular glands. The foveolas are covered by surface mucous cells and in the glands we have parietal cells, mucous neck cell and zymogenic cells. The rat´s gastric mucosa completes the maturation process during the third post-natal week, throughout the food transition process. At 14 th day, the pups begin to eat chow with maternal milk and at 21 st day the weaning, for experimental animals, is complete. The early weaning consists in the abrupt suckling interruption at 15 th day. This diet modification is related to accelerated maturation process of diverse organs, increase in mucous neck cells differentiation and in corticosterone levels (the main glucocorticoid hormone in rats). The glucocorticoids (GCs) action can be inhibited by RU486, a glucocorticoid receptor antagonist. The aim of this study was to evaluate if the increase in corticosterone levels triggered to early weaning could affect gastric epithelium maturation and to verify if these changes could persist to adult age. For that, we had four experimental groups: suckling, suckling-RU486, early weaning and early weaning-RU486. Using RT-qPCR, in 17-d-old rats, we observed that early weaning increased Muc5ac, Bhlha15 and Pgc and decreased Msn (comparing suckling-RU486 and early weaning-RU486) and Pga5 expression, while RU486 reverted the results for Pgc and Muc5ac, reduced Gif expression in early weaning-RU486, reduced Fut1 expresion in suckling-RU486 and increased Muc6 expression (genes related to gastric maturation process). The efficiency of RU486 treatment was attested by the reduction of SGK1 mRNA levels, which were elevated by early weaning. Mucous neck cells population was evaluated using the histochemistry reactions of PASAB and GSII lectin, and the immune-hystochemistry for mucin 6. In 17 and 18-d-old animals, we noted that early weaning increased mucous neck cells number as RU486 reverted this result. The zymogenic cells were analysed with immune-histochemistry reactions for Mist1 and for pepsinogen C (PGC). In pups, we found that early weaning increased Mist1-positive and PGC-positive cells populations, although corticosterone inhibition reverted only the result for PGC. In 30-d-old rats, we had the following results: reduction on Fut1 expression caused by early weaning; reduction on mRNA levels for moesin in SRU and early weaning groups, and elevation on early weaning-RU486; reduction on Pga5 expression provoked by RU486; the results over Pgc were maintained; the results, caused both by early weaning as RU486, over the population of mucous neck cells positives for PASAB and positives for mucin 6 were maintained, as well as the effects of RU486 over PGC immune-stained cells. We concluded that the increase in corticosterone levels, caused by early weaning, has a fundamental role in the mucous neck cells and zymogenic cells complete differentiation.

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Regulation of corticosterone function during early weaning and effects on gastric cell proliferation

Cited by 16 publications

References 48 publications

Does early weaning shape future endocrine and metabolic disorders? Lessons from animal models

Does early weaning shape future endocrine and metabolic disorders? Lessons from animal models

O desmame precoce aumenta e reprograma a diferenciação de células mucosas do colo em células zimogênicas na mucosa gástrica de ratos.

Diferenciação celular no epitélio gástrico de ratos submetidos ao desmame precoce: avaliação da ação da corticosterona.

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