Regulation of clusterin expression in mammary epithelial cells

Itahana, Yoko; Piens, Marie; Sumida, Tomoki; Fong, Sylvia; Muschler, John; Desprez, Pierre Yves

doi:10.1016/j.yexcr.2006.12.010

Cited by 24 publications

(15 citation statements)

References 38 publications

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“…A previous study has proposed that sCLU has a cytoprotective effect, whereas elevated levels of nCLU may trigger cell death (Criswell et al 2003). Overexpression of sCLU has been demonstrated in various malignancies, such as colorectal (Chen et al 2003), prostate (Pins et al 2004), breast (Itahara et al 2007), renal (Kurahashi et al 2005) and liver cancers (Kang et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Plasma proteome analysis of cervical intraepithelial neoplasia and cervical squamous cell carcinoma

Looi¹,

Karsani

Rahman

et al. 2009

J Biosci

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Although cervical cancer is preventable with early detection, it remains the second most common malignancy among women. An understanding of how proteins change in their expression during a particular diseased state such as cervical cancer will contribute to an understanding of how the disease develops and progresses. Potentially, it may also lead to the ability to predict the occurrence of the disease. With this in mind, we aimed to identify differentially expressed proteins in the plasma of cervical cancer patients. Plasma from control, cervical intraepithelial neoplasia (CIN) grade 3 and squamous cell carcinoma (SCC) stage IV subjects was resolved by two-dimensional gel electrophoresis and the resulting proteome profiles compared. Differentially expressed protein spots were then identified by mass spectrometry. Eighteen proteins were found to be differentially expressed in the plasma of CIN 3 and SCC stage IV samples when compared with that of controls. Competitive ELISA further validated the expression of cytokeratin 19 and tetranectin. Functional analyses of these differentially expressed proteins will provide further insight into their potential role(s) in cervical cancer-specific monitoring and therapeutics.

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Section: Discussionmentioning

confidence: 99%

Plasma proteome analysis of cervical intraepithelial neoplasia and cervical squamous cell carcinoma

Looi¹,

Karsani

Rahman

et al. 2009

J Biosci

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“…SGP-2/clusterin has diverse functions, including lipid transport, complement inhibition, and regulation of apoptosis (47). During mouse mammary gland development, SGP-2/clusterin is upregulated twice, at the end of pregnancy and at the beginning of the involution, when dramatic phenotypic and functional changes occur in the mammary gland (24). Although SGP-2/clusterin is associated with apoptosis during involution, there is accumulating evidence suggesting that it is an antiapoptotic protein associated with both cancer drug therapy resistance and cellular protection against exogenous stresses (36,48,49).…”

Section: Discussionmentioning

confidence: 99%

Mouse Mammary Tumor Virus Suppresses Apoptosis of Mammary Epithelial Cells through ITAM-Mediated Signaling

Kim¹,

Grande²,

Monroe³

et al. 2012

J Virol

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Many receptors in hematopoietic cells use a common signaling pathway that relies on a highly conserved immunoreceptor tyrosine-based activation motif (ITAM), which signals through Src family tyrosine kinases. ITAM-bearing proteins are also found in many oncogenic viruses, including the mouse mammary tumor virus (MMTV) envelope (Env). We previously showed that MMTV Env expression transformed normal mammary epithelial cells and that Src kinases were important mediators in this transformation. To study how ITAM signaling affects mammary cell transformation, we utilized mammary cell lines expressing two different ITAM-containing proteins, one encoding a MMTV provirus and the other a B cell receptor fusion protein. ITAMexpressing cells were resistant to both serum starvation-and chemotherapeutic drug-induced apoptosis, whereas cells transduced with these molecules bearing ITAM mutations were indistinguishable from untransduced cells in their sensitivity to these treatments. We also found that Src kinase was activated in the MMTV-expressing cells and that MMTV-induced apoptosis resistance was completely restored by the Src inhibitor PP2. In vivo, MMTV infection delayed involution-induced apoptosis in the mouse mammary gland. Our results show that MMTV suppresses apoptosis through ITAM-mediated Src tyrosine kinase signaling. These studies could lead to the development of effective treatment of nonhematopoietic cell cancers in which ITAM-mediated signaling plays a role.

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“…Previous studies have demonstrated that clusterin has a cytoprotective role against oxidants. 19 , 20 Clusterin has been reported as a multifunctional protein to prevent protein aggregation, to induce apoptosis of inflammatory cells as well as to inhibit complement. 20 , 21 In addition, clusterin as a sensor of oxidative and proteotoxic stress, it is implicated in numerous age-related diseases including neuro-degeneration, cardiovascular diseases, metabolic syndromes, inflammation, and cancer.…”

Section: Discussionmentioning

confidence: 99%

Serum Clusterin as a Prognostic Marker of Chronic Spontaneous Urticaria

Kim

Lee²,

Ban³

et al. 2016

Medicine

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A substantial proportion of patients with chronic spontaneous urticaria (CSU) are refractory to antihistamines. However, identifying the subpopulation whose urticaria is not completely controlled by antihistamines remains difficult. The response of autologous serum skin test (ASST), a clinical test for the detection of basophil histamine-releasing activity upon autoantibodies or autoreactive stimulation, has been suggested as a potential predictor in the control of urticaria. We sought to identify proteins that were differentially expressed in the sera of patients with positive and negative ASST results and to investigate their association with urticaria control.Proteomics analysis was performed using sera from 3 CSU patients with positive ASST results compared with those showing negative ASST results. Seven upregulated and 5 downregulated proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in the ASST-positive group compared with the ASST-negative group.Proteins that were differentially expressed according to the ASST results in CSU patients were classified into 6 groups: apolipoproteins, glycoproteins, modified albumin, haptoglobulin, plectin, and others. Among these, apolipoprotein J or clusterin was validated using an enzyme-linked immunosorbent assay. Clusterin levels in 69 ASST-positive patients were significantly higher than those in 69 ASST-negative patients and in 86 healthy controls (231.2 ± 44.0 vs 210.2 ± 36.1 vs 118.7 ± 71.9 μg/mL, P < 0.001). Furthermore, clusterin levels differed significantly between patients with responsive and refractory responses to antihistamine treatment within 3 months (231.0 ± 39.1 vs 205.1 ± 40.4 μg/mL, P < 0.001). ASST results and serum clusterin levels can predict 92.7% of CSU patients whose urticaria would be refractory to antihistamines. Serum clusterin can be a prognostic marker to determine the responsiveness to antihistamine treatment in patients with CSU.

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Regulation of clusterin expression in mammary epithelial cells

Cited by 24 publications

References 38 publications

Plasma proteome analysis of cervical intraepithelial neoplasia and cervical squamous cell carcinoma

Plasma proteome analysis of cervical intraepithelial neoplasia and cervical squamous cell carcinoma

Mouse Mammary Tumor Virus Suppresses Apoptosis of Mammary Epithelial Cells through ITAM-Mediated Signaling

Serum Clusterin as a Prognostic Marker of Chronic Spontaneous Urticaria

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