Regulation of ciliary differentiation of human respiratory epithelial cells by the receptor for hyaluronan-mediated motility on hyaluronan-based biomaterials

Huang, Tsung‐Wei; Cheng, Po‐Wen; Chan, Yen-Hui; Yeh, Te‐Huei; Young, Yi‐Ho; Young, Tai‐Horng

doi:10.1016/j.biomaterials.2010.05.054

Cited by 18 publications

(19 citation statements)

References 29 publications

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“…Accordingly, BRCA1 depletion increases the clonogenic potential of mammary epithelia [14]–[16], while a BRCA1 S308A mutant alters embryonic stem cell differentiation [71]. Moreover, RHAMM abundance may be central to the balance between AURKA-TPX2 and BRCA1-BARD1 activities during polarization; consistently, depletion of RHAMM also impairs ciliary differentiation of human respiratory epithelial cells [72]. However, key components of the depicted molecular wiring diagram are probably missing, such as a phosphatase that regulates AURKA-mediated modification of BRCA1 and RHAMM.…”

Section: Discussionmentioning

confidence: 99%

Interplay between BRCA1 and RHAMM Regulates Epithelial Apicobasal Polarization and May Influence Risk of Breast Cancer

Maxwell¹,

Benı́tez²,

Gómez-Baldó³

et al. 2011

PLoS Biol

123

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Section: Discussionmentioning

confidence: 99%

Interplay between BRCA1 and RHAMM Regulates Epithelial Apicobasal Polarization and May Influence Risk of Breast Cancer

Maxwell¹,

Benı́tez²,

Gómez-Baldó³

et al. 2011

PLoS Biol

123

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“…Researchers have suggested that HA has a pivotal role in mucosal host defence by stimulating the ciliary beating of nasal epithelial cells (NECs), and increasing the clearance of foreign material [7]. Recent clinical works have proposed several HA-based biomaterials to promote mucosal healing, reduce postoperative adhesion formation, prevent fibrosis and restore mucociliary epithelium of sinonasal mucosa [8][9][10]. However, no research has yet thoroughly investigated how HA influences the growth and differentiation of NECs, particularly the relationship between HA and TGF-b1.…”

Section: Introductionmentioning

confidence: 99%

Hyaluronan antagonizes the differentiation effect of TGF-β1 on nasal epithelial cells through down-regulation of TGF-β type I receptor

Huang

Fang

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Although hyaluronan (HA)-based biomaterials have been proposed to promote mucociliary differentiation of nasal epithelial cells (NECs), the mechanism by which HA affects the growth and differentiation of NECs has not been thoroughly explored. This study investigates the effect and mechanism of HA on the differentiation of NECs. The experiment cultures human NECs in four conditions, namely controls, transforming growth factor (TGF)-β1, TGF-β1 + HA and HA groups. In the TGF group, the NECs become irregular shape without formation of tight junction and mucociliary differentiation of NECs is inhibited. Epithelial-mesenchymal transition (EMT) of NECs also occurs in the TGF group. However, with addition of HA in TGF groups, NECs reveal the mucociliary phenotypes of epithelial cells with tight junction expression. Incubation of TGF-β1 in an NEC culture leads to an increase in phosphorylated type 1 TGF-β receptors (p-TβRI). This increase is attenuated when NECs are cultured in the presence of HA. Similar expressions are observed in phosphorylated smad2/smad3. Additionally, HA-dependent inhibition of TGF-β1 signalling is inhibited by co-incubation with a blocking antibody to CD44. Experimental results indicate that HA can antagonize TGF-β1 effect on EMT and mucociliary differentiation of NECs by down-regulation of TβR I, which is via CD44.

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“…The unique viscoelasticity and mucoadhesive capability of hyaluronic acid [14, 15] together with its high immunological and toxicological product safety have led to its versatile use in a number of application forms for various dermatological [16–22], pharmaceutical [3, 14, 23–32], and tissue engineering [33, 34] purposes, or during surgical procedures as well as for postoperative treatment [35–56]. …”

Section: Introductionmentioning

confidence: 99%

Clinical Efficacy of a Spray Containing Hyaluronic Acid and Dexpanthenol after Surgery in the Nasal Cavity (Septoplasty, Simple Ethmoid Sinus Surgery, and Turbinate Surgery)

2014

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Background. This prospective, controlled, parallel-group observational study investigated the efficacy of a spray containing hyaluronic acid and dexpanthenol to optimise regular treatment after nasal cavity surgery in 49 patients with chronic rhinosinusitis. Methods. The control group received standard therapy. Mucosal regeneration was determined using rhinoscopy sum score (RSS). Pre- and postoperative nasal patency was tested using anterior rhinomanometry. The participants were questioned about their symptoms. Results. Regarding all RSS parameters (dryness, dried nasal mucus, fibrin deposition, and obstruction), mucosal regeneration achieved good final results in both groups, tending to a better improvement through the spray application, without statistically significant differences during the whole assessment period, the mean values being 7.04, 5.00, 3.66, and 3.00 (intervention group) and 7.09, 5.14, 4.36, and 3.33 (control group). No statistically significant benefit was identified for nasal breathing, foreign body sensation, and average rhinomanometric volume flow, which improved by 12.31% (control group) and 11.24% (nasal spray group). Conclusion. The investigational product may have additional benefit on postoperative mucosal regeneration compared to standard cleaning procedures alone. However, no statistically significant advantage could be observed in this observational study. Double-blind, controlled studies with larger populations will be necessary to evaluate the efficacy of this treatment modality.

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Regulation of ciliary differentiation of human respiratory epithelial cells by the receptor for hyaluronan-mediated motility on hyaluronan-based biomaterials

Cited by 18 publications

References 29 publications

Interplay between BRCA1 and RHAMM Regulates Epithelial Apicobasal Polarization and May Influence Risk of Breast Cancer

Interplay between BRCA1 and RHAMM Regulates Epithelial Apicobasal Polarization and May Influence Risk of Breast Cancer

Hyaluronan antagonizes the differentiation effect of TGF-β1 on nasal epithelial cells through down-regulation of TGF-β type I receptor

Clinical Efficacy of a Spray Containing Hyaluronic Acid and Dexpanthenol after Surgery in the Nasal Cavity (Septoplasty, Simple Ethmoid Sinus Surgery, and Turbinate Surgery)

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