Regulation of Cercosporin Accumulation in Culture by Medium and Temperature Manipulation

Determination of a transcriptional regulator-like gene involved in biosynthesis of elsinochrome phytotoxin by the citrus scab fungus, Elsinoë fawcettii

“…Conidia were prepared using Fries medium (Fries, 1978) as described by Timmer et al (1996). Other media used in this study were complete medium (CM), minimal medium (MM) and protoplast regeneration medium (RMM) (Jenns et al, 1989;Chung et al, 2002). The pH of media was adjusted with 0.1 M phosphate buffer as described elsewhere (You et al, 2007).…”

“…Agar plugs bearing fungal mycelium were cut with a 6 mm sterilized cork borer, and soaked in 5 M KOH in the dark for at least 12 h. The A 480 of the soaking solution was measured using a model Genesys 5 spectrophotometer (Spectronic Instruments). Since elsinochromes and cercosporin have a similar structure and molecular mass (Weiss et al, 1987), elsinochromes were quantified using a molar absorption coefficient of 23 300 l mol 21 cm 21 (Jenns et al, 1989) and expressed as nmoles per plug. For TLC analysis, elsinochromes were extracted twice from dried agar medium with fungal mycelia in acetone for 16 h, condensed and separated on a TLC plate coated with 60 F254 fluorescent silica gel (5620 cm, Selecto Scientific) as previously described (Liao & Chung, 2008a, b).…”

Cercospora kikuchii aislada en la provincia de Santa Fe (Argentina): variabilidad genética y producción de cercosporina in vitro

“…La producción de cercosporina ha sido usada como un criterio para la predicción de patogenicidad [10]. La capacidad de un aislamiento de producir cercosporina in vitro depende de factores nutricionales y ambientales, principalmente de la temperatura y de la luz, aunque también del aislamiento evaluado [7].…”

“…[10]. A partir de cultivos de siete días, se inocularon placas conteniendo 10 ml de agar patata dextrosa.…”

Morphological and molecular identification of Cercospora apii on celery in Serbia

“…Međutim, navedene karakteristike ne mogu biti pouzdan taksonomski karakter za determinaciju vrsta roda Crecospora, jer su varijabilne (Jenns et al, 1989), a izgled i veličina konidija i kondiofora su veoma slične za mnoge vrste roda Cercospora (Welles, 1933). Pored toga, mnoge vrste ovog roda mogu kolonizirati tkiva primarno parazitirana od strane drugih vrsta, pri čemu se mogu izolovati sa atipičnih biljaka domaćina (Berger and Hanson, 1963;Groenewald et al, 2005;Groenewald et al, 2006).…”