Regulation of cellular responses to macroporous inorganic films prepared by the inverse-opal method

Orita, Toru; Tomita, Masahiro; Kato, Katsuya

doi:10.1016/j.colsurfb.2010.12.032

Cited by 22 publications

(31 citation statements)

References 33 publications

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“…It was also reported that macroporous membranes can influence the morphology of MC3T3-E1 cells, changing their square shape when cultured on non-porous membranes to elongated shape [43]. Contrary to what was observed with the myoblast cells, the P(VDF-TrFE) membranes will not be the most suitable for bone tissue engineering since these membranes promote a more elongated cell morphology.…”

Section: Cell Proliferation and Morphologymentioning

confidence: 93%

Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications

et al. 2015

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a b s t r a c tPoly(vinylidene fluoride) (PVDF) and its main copolymers -poly(vinylidene fluoride-cohexafluoropropene), P(VDF-HFP), and poly(vinylidene fluoride-co-trifluoroethylene), P(VDF-TrFE) -were processed by solvent casting at room temperature in the form of porous membranes. Copolymer membranes showed higher degree of porosity than PVDF, the average pore size being larger for P(VDF-TrFE) than for P(VDF-HFP) and PVDF. All membranes show high hydrophobicity with water contact angles in the range 94 to 115 , and electroactive beta phase contents above 90%. The adhesion and proliferation of both C2C12 myoblast and MC3T3-E1 pre-osteoblast cells on the membranes were investigated. It is demonstrated that PVDF membranes promote higher cell proliferation while P(VDF-HFP) membranes show the lowest proliferation for both kinds of cell. The proliferation on P(VDF-TrFE) membranes is cell dependent, higher for MC3T3-E1 cells but lower for C2C12 cells, related to the effect of the highly porous structure on the preferred morphology of each cell type, as the higher pore size and porosity of the P(VDF-TrFE) membrane induce cell elongation, which is preferred just by the C2C12 muscle cells.

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Section: Cell Proliferation and Morphologymentioning

confidence: 93%

Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications

et al. 2015

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“…Without adhesion, osteoblasts cannot spread or differentiate. A princeps issue in cell adhesion is the development of focal adhesions which, furthermore, are established sites for signal transduction, acting as conveyors of mechanical forces directly to the nucleus via the cytoskeletal network by the complex process of mechanotransduction, where vinculin is required for a strong network formation [1, 4, 44]. Based on the fact that the size and shape of cell spreading area, as well as the number and distribution of focal adhesion plaques, are decisive for migratory, proliferative, and differentiation behaviour of anchorage-dependent cells, we performed animage-based quantitative feature extraction design in which focal adhesion quantification has been used as marker to assess the osteoconductive potential of the substrata, together with actin cytoskeleton arrangement [2, 17, 20, 45].…”

Section: Discussionmentioning

confidence: 99%

Osteoconductive Potential of Barrier NanoSiO₂PLGA Membranes Functionalized by Plasma Enhanced Chemical Vapour Deposition

Terriza

Vilches-Pérez

Orden

et al. 2014

BioMed Research International

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The possibility of tailoring membrane surfaces with osteoconductive potential, in particular in biodegradable devices, to create modified biomaterials that stimulate osteoblast response should make them more suitable for clinical use, hopefully enhancing bone regeneration. Bioactive inorganic materials, such as silica, have been suggested to improve the bioactivity of synthetic biopolymers. An in vitro study on HOB human osteoblasts was performed to assess biocompatibility and bioactivity of SiO2 functionalized poly(lactide-co-glycolide) (PLGA) membranes, prior to clinical use. A 15 nm SiO2 layer was deposited by plasma enhanced chemical vapour deposition (PECVD), onto a resorbable PLGA membrane. Samples were characterized by X-ray photoelectron spectroscopy, atomic force microscopy, scanning electron microscopy, and infrared spectroscopy (FT-IR). HOB cells were seeded on sterilized test surfaces where cell morphology, spreading, actin cytoskeletal organization, and focal adhesion expression were assessed. As proved by the FT-IR analysis of samples, the deposition by PECVD of the SiO2 onto the PLGA membrane did not alter the composition and other characteristics of the organic membrane. A temporal and spatial reorganization of cytoskeleton and focal adhesions and morphological changes in response to SiO2 nanolayer were identified in our model. The novedous SiO2 deposition method is compatible with the standard sterilization protocols and reveals as a valuable tool to increase bioactivity of resorbable PLGA membranes.

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“…PS substrates with nanometer-sized features are commonly deployed biocompatible platforms which can act as a distinct in vitro environment for better manipulation and understanding of in vivo cell behavior [19], [20]. The wound has been created on the cells covering the working electrode by electric pulse and the healing process has been monitored online in terms of resistance and capacitance variation with time.…”

Section: C1mentioning

confidence: 99%

Real-Time Monitoring of Wound Healing on Nano-Patterned Substrates: Non-Invasive Impedance Spectroscopy Technique

Mondal

Bose

Chaudhuri

2016

IEEE Trans. Nanotechnology

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Regulation of cellular responses to macroporous inorganic films prepared by the inverse-opal method

Cited by 22 publications

References 33 publications

Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications

Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications

Osteoconductive Potential of Barrier NanoSiO₂PLGA Membranes Functionalized by Plasma Enhanced Chemical Vapour Deposition

Real-Time Monitoring of Wound Healing on Nano-Patterned Substrates: Non-Invasive Impedance Spectroscopy Technique

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Regulation of cellular responses to macroporous inorganic films prepared by the inverse-opal method

Cited by 22 publications

References 33 publications

Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications

Poly(vinylidene fluoride) and copolymers as porous membranes for tissue engineering applications

Osteoconductive Potential of Barrier NanoSiO2PLGA Membranes Functionalized by Plasma Enhanced Chemical Vapour Deposition

Real-Time Monitoring of Wound Healing on Nano-Patterned Substrates: Non-Invasive Impedance Spectroscopy Technique

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Product

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Osteoconductive Potential of Barrier NanoSiO₂PLGA Membranes Functionalized by Plasma Enhanced Chemical Vapour Deposition