Regulation of Cellular Calcium in Cardiac Myocytes

Reverse Mode of the Sarcoplasmic Reticulum Calcium Pump and Load-Dependent Cytosolic Calcium Decline in Voltage-Clamped Cardiac Ventricular Myocytes

“…We have used values that combine both approaches. That is, the sum of literature-based individual values behave as ensemble-like measurements of total cytosolic buffering in cardiac myocytes (Bers, 1999). These parameters (Table 2) include consideration of ligand binding kinetics and were defined at pH 7-7.2 and mostly at room temperature.…”

“…The data are much better described by this equation than by the Hill equation plus the leak function (not shown). Best-fit parameters are in Table 3. Isolated myocytes, however, tend to demonstrate smaller [Ca] SRT than other systems (skinned fiber, papillary muscle, perfused heart, and permeabilized myocytes as reviewed in Bers, 1999). The reason for this is unknown but by allowing B max to vary the data can be well described.…”