Regulation of cell growth-dependent expression of mammalian CDC6 gene by the cell cycle transcription factor E2F

Ohtani, Kiyoshi; Tsujimoto, Atsumi; Ikeda, Masa‐Aki; Nakamura, Masataka

doi:10.1038/sj.onc.1202105

Cited by 107 publications

(103 citation statements)

References 34 publications

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“…The recombinant adenovirus expressing human E2F1, Ad-E2F1 and the control virus, Ad-Con, have been described (Ohtani et al, 1998). Ad-12SE1A(d2-11) was generated from CMV-E1A d2-11 mutant (Alevizopoulos et al, 1998) using ViraPower Adenoviral Expression System (Invitrogen) according to the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

“…Northern blot analysis was performed as described (Ohtani et al, 1998). Human acidic ribosomal phosphoprotein P0 (ARPP) cDNA was the control probe.…”

Section: Northern Blottingmentioning

confidence: 99%

“…pHsMCM5-Luc(-1384), pHsCdc6-Luc(-570), p27 Kip1 reporters pGL3-844-S, pGL3-635-S and pGL3-499-S, expression vectors for E2F1 through E2F3, and b-galactosidase expression vector pCMV-b-gal have been described (Ohtani et al, 1998(Ohtani et al, , 1999Ito et al, 1999). pGL3-814-S and pGL3-788-S were generated by removing NheI-Bpu10I and NheI-ApaI region from pGL3-844-S, respectively.…”

Section: Plasmids and Reporter Assaymentioning

confidence: 99%

“…5 0 deletion mutants were generated using Deletion Kit for Kilo-sequencing (Takara). Transfection of REF52 cells and luciferase assays were performed as described (Ohtani et al, 1998). HFFs were transfected with reporter and expression plasmids by lipofection using FuGENE 6 (Roche Diagnostics), together with pCMV-b-gal as an internal control.…”

Section: Plasmids and Reporter Assaymentioning

confidence: 99%

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Identification of novel E2F1 target genes regulated in cell cycle-dependent and independent manners

et al. 2005

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The transcription factor E2F mediates cell cycle-dependent expression of genes important for cell proliferation in response to growth stimulation. To further understand the role of E2F, we utilized a sensitive subtraction method to explore new E2F1 targets, which are expressed at low levels and might have been unrecognized in previous studies. We identified 33 new E2F1-inducible genes, including checkpoint genes Claspin and Rad51ap1, and four genes with unknown function required for cell cycle progression. Moreover, we found three groups of E2F1-inducible genes that were not induced by growth stimulation. At least, two groups of genes were directly induced by E2F1, indicating that E2F1 can regulate expression of genes not induced during the cell cycle. One included Neogenin, WASF1 and SGEF genes, which may have a role in differentiation or development. The other was the cyclin-dependent kinase inhibitor p27 Kip1 , which was involved in suppression of inappropriate cell cycle progression induced by deregulated E2F. E2F1-responsive regions of these genes were located more upstream than those of typical E2F targets and did not have typical E2F sites. These results indicate that there are groups of E2F1 targets, which are regulated in a distinct manner from that of typical E2F targets.

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Section: Methodsmentioning

confidence: 99%

“…Northern blot analysis was performed as described (Ohtani et al, 1998). Human acidic ribosomal phosphoprotein P0 (ARPP) cDNA was the control probe.…”

Section: Northern Blottingmentioning

confidence: 99%

Section: Plasmids and Reporter Assaymentioning

confidence: 99%

Section: Plasmids and Reporter Assaymentioning

confidence: 99%

See 2 more Smart Citations

Identification of novel E2F1 target genes regulated in cell cycle-dependent and independent manners

et al. 2005

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show abstract

“…This gene cell cycledependent expression is due to two E2F binding sites located in close vicinity of the transcription initiation site (-43/-36 and -8/-1) and responsible for the transcriptional activity of the entire 4.5 Kb 5' untranscribed region in proliferating cells. [24][25][26] In contrast, the enhancer activity relevant to megakaryocytic endocycles lies in a distant region, at about 3.5 Kb upstream transcription initiation site. 12 Because all hematopoietic cells lines that differentiate toward megakaryocytes already express GATA1, it is difficult to assess its direct effect on a potential non-hematopoietic target.…”

Section: Cdc6 Has An Additional Important Rolementioning

confidence: 99%