Regulation of cell cycle and stress responses under nitrosative stress in Schizosaccharomyces pombe

Majumdar, Uddalak; Biswas, Pranjal; Sarkar, Tanmoy; Maiti, Debasish; Ghosh, Sudakshina

doi:10.1016/j.freeradbiomed.2012.03.026

Cited by 24 publications

(15 citation statements)

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“…In this study we added 100 mM detaNONOate solution in 0.01 N NaOH to 1 × PBS to a final concentration of 3 mM, 1 h prior to addition of cells and incubated at 30°C to obtain a good release of NO into the buffer solution. We previously reported that fission yeast S. pombe cells are much more sensitive to a concentration of 3 mM of detaNONOate than to 1 mM (Sahoo et al, 2006;Majumdar et al, 2012) in terms of cell growth, lowering the mitotic index, while cell viability is maintained at 95%. It can be clearly seen from Fig.…”

Section: Resultsmentioning

confidence: 99%

Global transcriptomic profiling of Schizosaccharomyces pombe in response to nitrosative stress

Biswas

Ghosh

2015

Gene

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Section: Resultsmentioning

confidence: 99%

Global transcriptomic profiling of Schizosaccharomyces pombe in response to nitrosative stress

Biswas

Ghosh

2015

Gene

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“…This hypothesis is corroborated by an increased phosphorylation of Cdk1 tyrosine 15 residue in a dose-dependent fashion by NaHS treatment that could reflect the inactivation of Cdc25, which could favor the inhibitory kinase of MPF, Myt1. Two more piece of evidence strengthen this hypothesis: (i) while it is accepted that S-nitrosylation and S-sulfhydration occur on the same proteins, S-nitrosylation of Cdc25C has been identified [60][61][62], (ii) a Cdc25 crucial cysteine residue is inactivated by ROS [63].…”

Section: Discussionmentioning

confidence: 96%

Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes

Gelaude

Slaby

Cailliau

et al. 2020

Cells

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The role of hydrogen sulfide (H2S) is addressed in Xenopus laevis oocytes. Three enzymes involved in H2S metabolism, cystathionine β-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an acceleration of oocyte meiosis resumption when inhibited. Moreover, meiosis resumption is associated with a significant decrease in endogenous H2S. On another hand, a dose-dependent inhibition was obtained using the H2S donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not induce the dissociation of the components of the M-phase promoting factor (MPF), cyclin B and Cdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-amplification loop were sensitive to H2S. Superoxide dismutase and catalase hindered the effects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of H2S signaling in the timing of amphibian oocytes meiosis resumption.

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“…p21 WAF1/CIP1 downregulation by NO was also reported in Xenopus embryos, but through nitric oxide modulation of the RhoA-ROCK pathway [18] (Figure 1B). Thus, though there are converging evidences for role of NO in cell cycle regulation [85], the exact mechanisms remain to be fully deciphered.…”

Section: Kip1mentioning

confidence: 99%

From Nitric Oxide Toward S-Nitrosylation: Expanding Roles in Gametes and Embryos

Ješeta¹,

Sedmı́ková²,

Bodart³

2017

Nitric Oxide Synthase - Simple Enzyme-Complex Roles

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Nitric oxide (NO) is a gasotransmitter involved in various aspects of reproduction. The observational data from different species, such as sea urchin, ascidians, amphibians, rodents, porcine, bovine, and human, suggest that NO might have a significant role in reproduction through several mechanisms. This proposed role might appear preserved through evolution; however, the effects of NO also depend on the species or stages considered. There has been debate over the physiological relevance of NO, though the benefits of its use in assisted reproduction are now widely recognized. Over the past years, S-nitrosylation has provided a new angle to decipher the mechanisms through which NO exerts its actions. This chapter summarizes, in a nonexhaustive manner, research that explores the role of NO in gametes and embryos.

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Regulation of cell cycle and stress responses under nitrosative stress in Schizosaccharomyces pombe

Cited by 24 publications

References 50 publications

Global transcriptomic profiling of Schizosaccharomyces pombe in response to nitrosative stress

Global transcriptomic profiling of Schizosaccharomyces pombe in response to nitrosative stress

Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes

From Nitric Oxide Toward S-Nitrosylation: Expanding Roles in Gametes and Embryos

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