Regulation of Cell Behavior by Hydrostatic Pressure

Liu, Shaobao; Tao, Ru; Wang, Ming; Tian, Jin; Genin, Guy M.; Lu, Tian Jian; Xu, Feng

doi:10.1115/1.4043947

Cited by 39 publications

(38 citation statements)

References 144 publications

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“…In contrast, some researchers reported reductions in cell viability under pressure, although the pressure applied was four times (4 kPa) that used in this study (30). Whether these discrepancies were due to the use of different species/cell types and/or methods is not known; however, a review of the various methods commonly used to apply hydrostatic pressure has shown differences in cell proliferation and viability due to cell type and amount loaded (38).…”

Section: Discussionmentioning

confidence: 56%

Hydrostatic pressure regulates CYP1A2 expression in human hepatocytes via a mechanosensitive aryl hydrocarbon receptor-dependent pathway

Burton

Scaife

Paine

et al. 2020

American Journal of Physiology-Cell Physiology

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Approximately 75% of xenobiotics are primarily eliminated through metabolism; thus the accurate scaling of metabolic clearance is vital to successful drug development. Yet, when data is scaled from in vitro to in vivo, hepatic metabolic clearance, the primary source of metabolism, is still commonly underpredicted. Over the past decades, with biophysics used as a key component to restore aspects of the in vivo environment, several new cell culture settings have been investigated to improve hepatocyte functionalities. Most of these studies have focused on shear stress, i.e., flow mediated by a pressure gradient. One potential conclusion of these studies is that hepatocytes are naturally “mechanosensitive,” i.e., they respond to a change in their biophysical environment. We demonstrate that hepatocytes also respond to an increase in hydrostatic pressure that, we suggest, is directly linked to the lobule geometry and vessel density. Furthermore, we demonstrate that hydrostatic pressure improves albumin production and increases cytochrome P-450 (CYP) 1A2 expression levels in an aryl hydrocarbon-dependent manner in human hepatocytes. Increased albumin production and CYP function are commonly attributed to the impacts of shear stress in microfluidic experiments. Therefore, our results highlight evidence of a novel link between hydrostatic pressure and CYP metabolism and demonstrate that the spectrum of hepatocyte mechanosensitivity might be larger than previously thought.

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Section: Discussionmentioning

confidence: 56%

Hydrostatic pressure regulates CYP1A2 expression in human hepatocytes via a mechanosensitive aryl hydrocarbon receptor-dependent pathway

Burton

Scaife

Paine

et al. 2020

American Journal of Physiology-Cell Physiology

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“…Existing HP bioreactors have preliminarily incorporated techniques regarding different HP conditions on cartilage formation and cartilage, and researchers may focus on the regulation of cellular behavior in HP research in the future. Further specific review of the role of hydrostatic forces in regulating cell behavior was provided by Liu et al [ 55 ].…”

Section: Considerations For Seeded Cells In Bioreactor Designmentioning

confidence: 99%

The Application of Bioreactors for Cartilage Tissue Engineering: Advances, Limitations, and Future Perspectives

et al. 2021

Stem Cells International

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Tissue engineering (TE) has brought new hope for articular cartilage regeneration, as TE can provide structural and functional substitutes for native tissues. The basic elements of TE involve scaffolds, seeded cells, and biochemical and biomechanical stimuli. However, there are some limitations of TE; what most important is that static cell culture on scaffolds cannot simulate the physiological environment required for the development of natural cartilage. Recently, bioreactors have been used to simulate the physical and mechanical environment during the development of articular cartilage. This review aims to provide an overview of the concepts, categories, and applications of bioreactors for cartilage TE with emphasis on the design of various bioreactor systems.

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“…This complete fluid environment (defined here as "full vessel" for ease of reference) affects oxygen diffusion and hydrostatic loading, which fundamentally regulate homeostasis, [40][41][42] embryonic development [43][44][45] and key cellular processes such as differentiation, migration and proliferation. [46][47][48] Rotation of a culture vessel on clinostat, RPM or RWV devices induces fluid shear stresses and improves mass transport, which stimulate cell growth as in bioreactor culture. 49,50 Furthermore, simulated microgravity devices introduce system-specific artifacts that can affect cellular processes, such as centrifugal forces and vibrations (clinostats, RPMs, RWVs) 6 or magnetic fields (magnetic levitation devices) 51 but are seldom discussed.…”

Section: F I G U R Ementioning

confidence: 99%

“…Other than gravitational revectoring, s‐μg culture already presents a novel cellular environment compared to standard techniques in that the culture vessel must be completely filled with culture medium in order to reduce the effects of shear during rotationally‐mediated gravitational revectoring. This complete fluid environment (defined here as “full vessel” for ease of reference) affects oxygen diffusion and hydrostatic loading, which fundamentally regulate homeostasis, 40‐42 embryonic development 43‐45 and key cellular processes such as differentiation, migration and proliferation 46‐48 . Rotation of a culture vessel on clinostat, RPM or RWV devices induces fluid shear stresses and improves mass transport, which stimulate cell growth as in bioreactor culture 49,50 .…”

Section: Introductionmentioning

confidence: 99%

Factors implicating the validity and interpretation of mechanobiology studies in simulated microgravity environments

Poon

2020

Engineering Reports

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Simulated microgravity (s-μg) devices provide unique conditions for elucidating the effects of gravitational unloading on biological processes and are increasingly being applied for mechanobiology studies. However, without proper characterization of the mechanical environment generated by these systems, the interpretation of results is confounded and limited. Furthermore, the cell culture approaches central to s-μg experimentation introduces new factors that can fundamentally affect results, but these are currently not addressed. It is essential to understand the complete culture environment and how constituent conditions can individually and synergistically affect cellular responses in order to correctly interpret results, otherwise outcomes may be misattributed to the effects of microgravity alone. For the benefit of the growing space biology community, this article critically reviews a typical s-μg cell culture environment in terms of three key conditions: fluid-mediated mechanical stimuli, oxygen tension and biochemical. These and the implications of other experimental variables for biological analysis are categorically discussed. A new set of controls is proposed to properly evaluate the respective effects of these conditions in s-μg culture, along with a reporting matrix and potential strategies for addressing the current limitations of simulated microgravity devices as a platform for mechanobiology research.

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Regulation of Cell Behavior by Hydrostatic Pressure

Cited by 39 publications

References 144 publications

Hydrostatic pressure regulates CYP1A2 expression in human hepatocytes via a mechanosensitive aryl hydrocarbon receptor-dependent pathway

Hydrostatic pressure regulates CYP1A2 expression in human hepatocytes via a mechanosensitive aryl hydrocarbon receptor-dependent pathway

The Application of Bioreactors for Cartilage Tissue Engineering: Advances, Limitations, and Future Perspectives

Factors implicating the validity and interpretation of mechanobiology studies in simulated microgravity environments

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