Regulation of CD44–Hyaluronan Interactions in Burkitt's Lymphoma and Epstein–Barr Virus-Transformed Lymphoblastoid B Cells by PMA and Interleukin-4

Kryworuchko, Marko; Gee, Katrina; Díaz‐Mitoma, Francisco; Kumar, Ashok

doi:10.1006/cimm.1999.1491

Cited by 10 publications

(5 citation statements)

References 56 publications

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“…Although most cells express some form of CD44, not all cells constitutively bind to HA. 26,27 Functionally active HA-adhesive CD44 is produced through induction of sialidase via MAPK activation.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…HA interactions with CD44 and CD168 (RHAMM) can induce numerous cell behaviors, including activation of tyrosine kinases, protein kinase C, FAK, Pi3K, mitogen-activated protein kinases (MAPK), NFκB, and RAS, as well as cytoskeletal components required for inflammation and cancer. Although most cells express some form of CD44, not all cells constitutively bind to HA. , Functionally active HA-adhesive CD44 is produced through induction of sialidase via MAPK activation.…”

Section: Resultsmentioning

confidence: 99%

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Iminosugar idoBR1 Isolated from Cucumber Cucumis sativus Reduces Inflammatory Activity

Nash¹,

Bartholomew²,

Penkova³

et al. 2020

ACS Omega

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Cucumbers have been anecdotally claimed to have anti-inflammatory activity for a long time, but the active principle was not identified. idoBR1, (2R,3R,4R,5S)-3,4,5-trihydroxypiperidine-2-carboxylic acid, is an iminosugar amino acid isolated from fruits of certain cucumbers, Cucumis sativus (Cucurbitaceae). It has no chromophore and analytically behaves like an amino acid making detection and identification difficult. It has anti-inflammatory activity reducing lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-α) in THP-1 cells and ex vivo human blood. It showed selective inhibition of human α-l-iduronidase and sialidases from both bacteria (Tannerella forsythia) and human THP-1 cells. idoBR1 and cucumber extract reduced the binding of hyaluronic acid (HA) to CD44 in LPS-stimulated THP-1 cells and may function as an anti-inflammatory agent by inhibiting induced sialidase involved in the production of functionally active HA adhesive CD44. Similar to the related iminosugars, idoBR1 is excreted unchanged in urine following consumption. Its importance in the diet should be further evaluated.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Iminosugar idoBR1 Isolated from Cucumber Cucumis sativus Reduces Inflammatory Activity

Nash¹,

Bartholomew²,

Penkova³

et al. 2020

ACS Omega

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“…Viral DNA was isolated using a monophase solution containing guanidine thiocyanate and phenol (TRI Reagent solution, Molecular Research Center Inc., Cincinnati, OH, USA) as described [27]. HSV in the DNA isolated from clinical samples was detected by PCR analysis using HSV‐1 and HSV‐2 primers, as previously described [3].…”

Section: Methodsmentioning

confidence: 99%

Dysregulated expression of IFN-γand IL-10 and impaired IFN-γ-mediated responses at different disease stages in patients with genital herpes simplex virus-2 infection

Singh

Kumar

Creery

et al. 2003

Clinical and Experimental Immunology

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SUMMARY Cell‐mediated T‐helper type‐1 (Th1) responses play a vital role in the immunopathogenesis of genital infections caused by herpes simplex virus 2 (HSV‐2). We investigated the role of Th responses in HSV‐2 infection at different disease stages by analysing the production of Th cytokines in HSV‐stimulated peripheral blood mononuclear cells (PBMCs). IFN‐γ production decreased over time following a recurrence, whereas levels of IL‐10, and to a lesser extent IL‐2, remained elevated during this period. In addition, PBMCs from asymptomatic seropositive individuals produced high levels of IFN‐γ and low levels of IL‐10, in contrast to individuals with a history of genital ulcers. Following a recurrence, virus copy number in the genital lesions decreased progressively over time, in a manner similar to IFN‐γ production by HSV‐2‐stimulated PBMCs. Enhanced production of IFN‐γ may modulate HSV replication and B7 expression on monocytic cells of HSV‐infected individuals. In contrast to seronegative controls, IFN‐γ failed to enhance B7 expression on monocytic cells of HSV‐infected individuals. In addition, monocytic cells from HSV‐2‐infected individuals with recurrent disease supported greater HSV replication than did those of HSV‐infected asymptomatic individuals or seronegative controls. Furthermore, addition of IFN‐γ resulted in enhanced HSV replication in monocytic cells of HSV‐infected individuals with recurrent disease, in contrast to the inhibition observed in HSV‐seropositive asymptomatic individuals and seronegative controls. Taken together, our results suggest that dysregulated production of IFN‐γ at different disease stages and the impaired ability of monocytic cells to respond to IFN‐γ may play a role in the pathogenesis of recurrent genital herpes disease.

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“…However, the treatment EBV+ BL cells with B cell mitogen phorbol 12-myristate 13-acetate (PMA) or cytokine IL-4 enhances expression of an isoform H of CD44 and induces strong HA recognition in the cells. The ability to recognize HA was not observed in B-LCL cells stimulated with either PMA or IL-4 suggesting selective inactivation of molecular pathways that regulate CD44 expression and CD44-mediated HA binding in LCL cells [ 34 ]. Introduction of EBV latent membrane protein I (LMP1) gene into BL cells induces expression of CD44 on the cell surface suggesting that expression of LMP1 may regulate expression of CD44 and play a role in the behavior of EBV-based lymphomas [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Proteoglycan expression correlates with the phenotype of malignant and non-malignant EBV-positive B-cell lines

et al. 2015

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The involvement of proteoglycans (PGs) in EBV-host interactions and lymphomagenesis remains poorly investigated. In this study, expression of major proteoglycans (syndecan-1, glypican-1, perlecan, versican, brevican, aggrecan, NG2, serglycin, decorin, biglycan, lumican, CD44), heparan sulphate (HS) metabolic system (EXT1/2, NDST1/2, GLCE, HS2ST1, HS3ST1/2, HS6ST1/2, SULF1/2, HPSE) and extracellular matrix (ECM) components (collagen 1A1, fibronectin, elastin) in primary B cells and EBV carrying cell lines with different phenotypes, patterns of EBV-host cell interaction and viral latency stages (type I-III) was investigated. Primary B cells expressed a wide repertoire of PGs (dominated by serglycin and CD44) and ECM components. Lymphoblastoid EBV+ B cell lines (LCLs) showed specific PG expression with down-regulation of CD44 and ECM components and up-regulation of serglycin and perlecan/HSPG2. For Burkitt's lymphoma cells (BL), serglycin was down-regulated in BL type III cells and perlecan in type I BL cells. The biosynthetic machinery for HS was active in all cell lines, with some tendency to be down-regulated in BL cells. 5′-aza-dC and/or Trichostatin A resulted in transcriptional upregulation of the genes, suggesting that low expression of ECM components, proteoglycan core proteins and HS biosynthetic system is due to epigenetic suppression in type I cells. Taken together, our data show that proteoglycans are expressed in primary B lymphocytes whereas they are not or only partly expressed in EBV-carrying cell lines, depending on their latency type program.

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Regulation of CD44–Hyaluronan Interactions in Burkitt's Lymphoma and Epstein–Barr Virus-Transformed Lymphoblastoid B Cells by PMA and Interleukin-4

Cited by 10 publications

References 56 publications

Iminosugar idoBR1 Isolated from Cucumber Cucumis sativus Reduces Inflammatory Activity

Iminosugar idoBR1 Isolated from Cucumber Cucumis sativus Reduces Inflammatory Activity

Dysregulated expression of IFN-γand IL-10 and impaired IFN-γ-mediated responses at different disease stages in patients with genital herpes simplex virus-2 infection

Proteoglycan expression correlates with the phenotype of malignant and non-malignant EBV-positive B-cell lines

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