Regulation of cathepsin B and caspase-3 in long-term plasticity

Kudryashova, I. V.; Онуфриев, М. В.; Kudryashov, I. E.; Gulyaeva, N. V.

doi:10.1134/s1819712409040047

Cited by 3 publications

(5 citation statements)

References 32 publications

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“…In contrast, in our study of zebrafish, expression of three syntaxin genes differed among strains but was not associated with domestication history. Likewise, caspases, such as capase-3, are associated with synaptic plasticity and learning, in addition to their involvement in apoptosis [ 96 , 97 ]. Caspase-3a and capase-8 were up-regulated in domesticated populations of zebrafish (current study) and rainbow trout [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

Brain transcriptome variation among behaviorally distinct strains of zebrafish (Danio rerio)

et al. 2012

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BackgroundDomesticated animal populations often show profound reductions in predator avoidance and fear-related behavior compared to wild populations. These reductions are remarkably consistent and have been observed in a diverse array of taxa including fish, birds, and mammals. Experiments conducted in common environments indicate that these behavioral differences have a genetic basis. In this study, we quantified differences in fear-related behavior between wild and domesticated zebrafish strains and used microarray analysis to identify genes that may be associated with this variation.ResultsCompared to wild zebrafish, domesticated zebrafish spent more time near the water surface and were more likely to occupy the front of the aquarium nearest a human observer. Microarray analysis of the brain transcriptome identified high levels of population variation in gene expression, with 1,749 genes significantly differentially expressed among populations. Genes that varied among populations belonged to functional categories that included DNA repair, DNA photolyase activity, response to light stimulus, neuron development and axon guidance, cell death, iron-binding, chromatin reorganization, and homeobox genes. Comparatively fewer genes (112) differed between domesticated and wild strains with notable genes including gpr177 (wntless), selenoprotein P1a, synaptophysin and synaptoporin, and acyl-CoA binding domain containing proteins (acbd3 and acbd4).ConclusionsMicroarray analysis identified a large number of genes that differed among zebrafish populations and may underlie behavioral domestication. Comparisons with similar microarray studies of domestication in rainbow trout and canids identified sixteen evolutionarily or functionally related genes that may represent components of shared molecular mechanisms underlying convergent behavioral evolution during vertebrate domestication. However, this conclusion must be tempered by limitations associated with comparisons among microarray studies and the low level of population-level replication inherent to these studies.

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Section: Resultsmentioning

confidence: 99%

Brain transcriptome variation among behaviorally distinct strains of zebrafish (Danio rerio)

et al. 2012

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“…Furthermore, these modifications have been reported in some forms of long term plasticity [11][12][13]. We also observed a dependence of the amplitude of the presynaptic com ponent on the activity of caspase 3 [14]. In the major ity of slices, synaptic potentiation was accompanied by a decrease in the amplitude of the presynaptic compo nent to 88 ± 3% on average.…”

Section: Resultsmentioning

confidence: 49%

“…We believe that this effect was related to neurodegenerative changes in sin gle afferent terminals [14]. However, in the group of slices that were subjected to high frequency stimula tion we did not reveal any sign of caspase 3 activation (r = 0.18, p = 0.56) in spite of the significant decrease in the amplitude of the presynaptic component.…”

Section: Resultsmentioning

confidence: 80%

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The variation of caspase-3 activity in rat hippocampal slices depends on the duration of LTP maintenance

2012

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The changes in the activities of proteases after the induction of CA1 long term synaptic plasticity in hippocampal slices from Wistar rats were investigated in the period of LTP maintenance. Electrophysio logical testing was performed for 1 h after high frequency stimulation (HFS, 100 Hz, 1 s) of Schaffer collat erals. The same testing paradigm was applied to control slices from the same animals, except for HFS. After all testing procedures, the slices were immediately frozen at -70°C and the activities of caspase 3 and calpain were measured in each slice separately. At 1 hour after HFS, caspase 3 activity decreased significantly com pared to control slices. During this period, complete or partial depotentiation was observed in these slices and the percentage of recovery significantly correlated with caspase 3 activity. Moreover, decreased caspase 3 activity was observed in the slices with complete depotentiation. We suggest that HFS induced caspase 3 inhibition may be explained by the attenuation of degenerative processes under sufficient afferent inflow. However, in the slices that were subjected to the same afferent stimulation, in which LTP maintenance was observed, caspase 3 activity was significantly higher. Thus, long term synaptic alterations seem to be associ ated with recurrent caspase 3 activation. If we exclude the influence of synaptic potentiation, practically no correlation exists between caspase 3 activity and population spike amplitude during the phase of LTP main tenance. In the majority of slices, some trend to presynaptic fiber volley depression developed in parallel but independently of synaptic potentiation. In contrast, we did not reveal any calpain effects on the indices we studied. Our results suggest that different proteolytic enzymes may have specific roles under the conditions of long term synaptic plasticity.

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“…However, caspase 3 is also involved in normal plasticity. This enzyme not only plays a role in the apo ptosis of neurons but also participates in synaptic plas ticity [41][42][43][44][45][46], particularly in synapse degeneration [47]. Afferent activation influences the state of these proteolytic systems and limits proteolysis of synaptic proteins.…”

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Structural and functional modifications of presynaptic afferents: Do they correlate with learning mechanisms?

Kudryashova

2011

Neurochem. J.

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We review the modern data on the involvement of structural modifications of presynaptic afferents during the acquisition of individual experiences. During the ontogeny or regeneration of nervous tissue, this mechanism is responsible for adjusting the architecture of neuronal ensembles to the demands of adaptive behavior. The molecular basis of the formation and elimination of additional axonal branches is the modifi cation of the actin cytoskeleton. These modifications involve cell adhesion molecules, as well as extracellular and intracellular signal and regulatory proteins, most of which play an important role in long term plasticity. The possible involvement of structural modifications of presynaptic afferents in long term memory consoli dation is discussed.

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Regulation of cathepsin B and caspase-3 in long-term plasticity

Cited by 3 publications

References 32 publications

Brain transcriptome variation among behaviorally distinct strains of zebrafish (Danio rerio)

Brain transcriptome variation among behaviorally distinct strains of zebrafish (Danio rerio)

The variation of caspase-3 activity in rat hippocampal slices depends on the duration of LTP maintenance

Structural and functional modifications of presynaptic afferents: Do they correlate with learning mechanisms?

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