Regulation of Carbon and Nitrogen Metabolism in the Unicellular Cyanobacteria Synechococcus spp.

Marsac, Nicole Tandeau de; Lee, Hyun-Mi

doi:10.1007/978-1-4615-4827-0_62

Cited by 8 publications

(7 citation statements)

References 71 publications

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“…Prochlorococcus transporters for alternative N sources are thus upregulated in an all‐or‐none manner, suggesting that the cell lacks the ability to specifically identify ambient N sources. As with other cyanobacteria, Prochlorococcus likely perceives alternative N sources as a reduction in the rate of N assimilation, perhaps via 2‐oxoglutarate (Forchhammer, 1999; Tandeau de Marsac and Lee, 1999), and thus responds by activating the transport of all N sources simultaneously.…”

Section: Resultsmentioning

confidence: 99%

“…Likened to the central processing unit (CPU) of the cell (Ninfa and Atkinson, 2000), the signal transducer P II has been shown in other cyanobacteria to coordinate the cellular carbon and nitrogen balance. Similar to NtcA, P II responds to levels of 2‐oxoglutarate (Forchhammer, 1999; Tandeau de Marsac and Lee, 1999). Increases in 2‐oxoglutarate levels enhance P II phosphorylation (Forchhammer and Hedler, 1997).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Global gene expression of Prochlorococcus ecotypes in response to changes in nitrogen availability

Tolonen

Aach

Lindell

et al. 2006

Molecular Systems Biology

139

186

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Nitrogen (N) often limits biological productivity in the oceanic gyres where Prochlorococcus is the most abundant photosynthetic organism. The Prochlorococcus community is composed of strains, such as MED4 and MIT9313, that have different N utilization capabilities and that belong to ecotypes with different depth distributions. An interstrain comparison of how Prochlorococcus responds to changes in ambient nitrogen is thus central to understanding its ecology. We quantified changes in MED4 and MIT9313 global mRNA expression, chlorophyll fluorescence, and photosystem II photochemical efficiency (F v /F m ) along a time series of increasing N starvation. In addition, the global expression of both strains growing in ammonium-replete medium was compared to expression during growth on alternative N sources. There were interstrain similarities in N regulation such as the activation of a putative NtcA regulon during N stress. There were also important differences between the strains such as in the expression patterns of carbon metabolism genes, suggesting that the two strains integrate N and C metabolism in fundamentally different ways.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Global gene expression of Prochlorococcus ecotypes in response to changes in nitrogen availability

Tolonen

Aach

Lindell

et al. 2006

Molecular Systems Biology

139

186

View full text Add to dashboard Cite

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“…Why do cyanobacteria measure the nitrogen status by sensing 2-oxoglutarate instead of glutamine? 2-Oxoglutarate is produced in the oxidative branch of the TCA-cycle, which in cyanobacteria has solely an anabolic function, delivering precursors for various biosynthetic reactions (for review see [89]). One of the main routes of newly fixed CO 2 is the synthesis of 2-oxoglutarate to produce glutamate via the GS-GOGAT cycle.…”

Section: P II Phosphorylation and 2-oxoglutarate Signallingmentioning

confidence: 99%

Global carbon/nitrogen control by P_IIsignal transduction in cyanobacteria: from signals to targets

2004

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PII signal transduction plays a pervasive role in microbial nitrogen control. Different phylogenetic lineages have developed various signal transduction schemes around the highly conserved core of the signalling system, which consists of the PII proteins. Among all various bacterial PII signalling systems, the one in cyanobacteria is so far unique: in unicellular strains, the mode of covalent modification is by serine phosphorylation and the interpretation of the cellular nitrogen status occurs by measuring the 2-oxoglutarate levels. Recent advances have been the identification of the phospho-PII phosphatase, the resolution of the crystal structure of PII proteins from Synechococcus and Synechocystis strains and the identification of novel functions of PII regulation in cyanobacteria, which highlight the central role of PII signalling for the acclimation to changing carbon-nitrogen regimes.

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“…2‐OG level, alone or accompanied by glutamine (Gln), allows cells to sense carbon and nitrogen status and to regulate various physiological processes. In the early studies, 2‐OG and Gln had been reported as antagonistic signals involved in regulation of GS gene transcription in Escherichia coli (Jiang et al 1998) and of nitrate uptake and assimilation in Cyanobacteria (Tandeau de Marsac and Lee 1999). Further experiments revealed that a signal transduction protein (PII) was a key element in these sensing systems, and a gene encoding a homologue of the bacterial PII protein has been isolated from Arabidopsis thaliana (Hsieh et al 1998) as well as rice plants (Sugiyama et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Regulation of carbon and nitrogen metabolisms in rice roots by 2‐oxoglutarate at the level of hexokinase

Yuan

Wang

et al. 2007

Physiologia Plantarum

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Feeding experiments were designed, to investigate the role of 2‐oxoglutarate (2‐OG) in regulation of carbon and nitrogen metabolisms in non‐photosynthetic tissues of rice (Oryza sativa L.), and enzyme activities involved in the metabolisms as well as contents of several relating metabolites were determined in the roots. The enhancement of 2‐OG level by feeding 2‐OG or metabolizable sugars [sucrose (Suc) or glucose (Glc)], rather than by feeding non‐metabolizable carbon sources (mannose or mannitol), led to increase in enzyme activities, including hexokinase (HXK, EC 2.7.1.1), nicotinamide adenine dinucleotide phosphate (NADP)+‐dependent isocitrate dehydrogenase (NADP+‐ICDH, EC 1.1.1.42), phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31), glutamine synthetase (GS, EC 6.3.1.2) and the reduced form of nicotinamide adenine dinucleotide (NADH)‐dependent glutamate synthase (NADH‐GOGAT, EC 1.4.1.14). In addition, the increase in ammonium uptake, glutamine and glutamate (Glu) as well as the decrease in soluble carbohydrates were observed. The effects of feeding 2‐OG or metabolizable sugars were reversed by feeding of N‐acetyl‐glucosamine (NAG; a HXK inhibitor). The decreased 2‐OG level by the feeding of NAG alone led to increase in soluble carbohydrates and decrease in the enzyme activities, ammonium uptake as well as Glu content. The effects of NAG were reversed by supply of 2‐OG, Suc and Glc. These results suggest that nitrogen uptake and assimilation as well as their related carbohydrate metabolism in rice roots were regulated in coordination by 2‐OG level, and HXK activity was involved in the regulation of 2‐OG.

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Regulation of Carbon and Nitrogen Metabolism in the Unicellular Cyanobacteria Synechococcus spp.

Cited by 8 publications

References 71 publications

Global gene expression of Prochlorococcus ecotypes in response to changes in nitrogen availability

Global gene expression of Prochlorococcus ecotypes in response to changes in nitrogen availability

Global carbon/nitrogen control by P_IIsignal transduction in cyanobacteria: from signals to targets

Regulation of carbon and nitrogen metabolisms in rice roots by 2‐oxoglutarate at the level of hexokinase

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Regulation of Carbon and Nitrogen Metabolism in the Unicellular Cyanobacteria Synechococcus spp.

Cited by 8 publications

References 71 publications

Global gene expression of Prochlorococcus ecotypes in response to changes in nitrogen availability

Global gene expression of Prochlorococcus ecotypes in response to changes in nitrogen availability

Global carbon/nitrogen control by PIIsignal transduction in cyanobacteria: from signals to targets

Regulation of carbon and nitrogen metabolisms in rice roots by 2‐oxoglutarate at the level of hexokinase

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Global carbon/nitrogen control by P_IIsignal transduction in cyanobacteria: from signals to targets