Regulation of bovine oviductal NO synthesis by follicular steroids and prostaglandins

Kobayashi, Yoshihiko; Yamamoto, Yuki; Kageyama, Soichi; Hirayama, Hiroki; Kimura, Koji; Okuda, Kiyoshi

doi:10.1530/rep-15-0254

Cited by 10 publications

(9 citation statements)

References 58 publications

(37 reference statements)

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“…This signaling pathway is upregulated during pregnancy and is mainly responsible for spontaneous oviduct muscle contraction, which provides a positive factor to the embryo movement toward the uterus (Ning et al 2014). Nitric oxide has been identified in the Fallopian tube; it mediates tubal muscle contraction, with possible roles in the regulation of sperm motility (Ekerhovd et al 1997, Kobayashi et al 2016. Dimethylarginine dimethylaminohydrolase 2 (DDAH2), an enzyme regulating nitric oxide synthesis, is also expressed in the oviduct in the presence of egg and embryo (Georgiou et al 2005).…”

Section: Gas In the Oviductmentioning

confidence: 99%

Oviduct: roles in fertilization and early embryo development

Winuthayanon

2017

Journal of Endocrinology

185

187

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Animal oviducts and human Fallopian tubes are a part of the female reproductive tract that hosts fertilization and pre-implantation development of the embryo. With an increasing understanding of roles of the oviduct at the cellular and molecular levels, current research signifies the importance of the oviduct on naturally conceived fertilization and pre-implantation embryo development. This review highlights the physiological conditions within the oviduct during fertilization, environmental regulation, oviductal fluid composition and its role in protecting embryos and supplying nutrients. Finally, the review compares different aspects of naturally occurring fertilization and assisted reproductive technology (ART)-achieved fertilization and embryo development, giving insight into potential areas for improvement in this technology.

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Section: Gas In the Oviductmentioning

confidence: 99%

Oviduct: roles in fertilization and early embryo development

Winuthayanon

2017

Journal of Endocrinology

185

187

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“…In the oviduct, several factors are known to activate NF-κB signaling (Gabler et al 2008, Shaw et al 2011. Our previous findings that iNOS mRNA expression is highest on the day of ovulation in the ampullary region during the estrous cycle (Kobayashi et al 2016) could be an evidence that EDNRB mediates iNOS expression/NO production via NF-κB in the oviduct. Other isoforms of NOS, eNOS , and frequency (E) of contractile motility in the ampulla (solid lines, n = 5) and isthmus (dashed lines, n = 6).…”

Section: Discussionmentioning

confidence: 92%

Roles of EDNs in regulating oviductal NO synthesis and smooth muscle motility in cows

Kobayashi¹,

Yoshimoto²,

Yamamoto³

et al. 2016

Reproduction

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Endothelins (EDNs) participate in various physiological events including smooth muscle contraction, nitric oxide (NO) synthesis, and embryonic development. In this study, we investigated the regional roles of EDNs produced by bovine oviductal epithelial cells in NO synthesis and smooth muscle motility. Quantification of mRNA expressions indicated that expression of EDN receptor B (EDNRB) in the ampullary region was higher after ovulation than before ovulation, whereas expression of EDNRA in the isthmic region was higher after ovulation than before ovulation. Immunohistochemistry revealed that the EDN receptors (EDNRA and EDNRB) were expressed in the epithelium, whereas smooth muscle showed positive staining only for EDNRA. The expressions of inducible NO synthase (iNOS) protein and its mRNA (NOS2) in cultured epithelial cells isolated from the ampulla were stimulated by EDN1, but not by EDN2 or EDN3, after 1 h of incubation. In isthmic epithelial cells, none of the EDNs affected the expression of NOS2. Isometric contraction tests indicated that spontaneous waves were strong in the isthmic region but weak in the ampullary region. EDN1 modulated smooth muscle motility in both the regions. The overall findings suggest that EDN1 plays region-specific roles in smooth muscle motility and epithelial NO synthesis, providing an optimal oviductal microenvironment for transport of gametes, fertilization, and development/transport of early embryo.Reproduction (2016) 151 615-622

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“…EDN1 and EDN2 bind to both EDNRA and EDNRB, while EDN3 only binds to EDNRB (Bridges, Cho, & Ko, ). EDNRA (but not EDNRB) is expressed in bovine oviductal smooth muscle cells (Kobayashi, Yamamoto, et al., ; Kobayashi, Yoshimoto, et al., ). In various tissues, EDNRA is involved in contraction of smooth muscle (Al‐Alem et al., ; Rosselli et al., ; Sakamoto et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…G‐15 was pre‐incubated with the cells for 1 hr before incubation with E2. The doses of E2 was based on the E2 concentration in follicular fluid (Kobayashi, Yamamoto, et al., ; Kobayashi, Yoshimoto, et al., ), and that of G‐1 and G‐15 were based on the concentrations in previous reports (Bologa et al., ; Dennis et al., ).…”

Section: Methodsmentioning

confidence: 99%

“…These studies indicate that the expressions of EDNs and NOS in the bovine oviduct are controlled by oestradiol‐17β (Ε2), which is a major steroid hormone produced by ovarian follicles. We previously reported that bovine follicular fluid contains a high concentration of E2 (approximately 250 nmol/L) (Kobayashi, Yamamoto, et al., ; Kobayashi, Yoshimoto, et al., ). At the time of ovulation, follicular fluid enters oviduct with an oocyte (Ezzati, Djahanbakhch, Arian, & Carr, ; Hansen, Srikandakumar, & Downey, ).…”

Section: Introductionmentioning

confidence: 99%

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Acute stimulation of a smooth muscle constrictor by oestradiol‐17β via GPER1 in bovine oviducts

Nishie

Kobayashi

Kimura

et al. 2017

Reprod Domestic Animals

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Oviducts play roles in reproductive processes, including gametes transport, fertilization and early embryo development. Oviductal transport is controlled by various factors such as endothelins (EDNs) and nitric oxide (NO), smooth muscle contracting and relaxing factor, respectively. EDNs and NO production depend on an ovarian steroid hormone, oestradiol-17β (E2) and E2 quickly exerts their biological functions through G protein-coupled oestrogen receptor 1 (GPER1), which mediates rapid intracellular signalling. Because follicular fluid which contains a high concentration of E2 enters the oviduct, we hypothesized that E2 in the follicular fluid participates via GPER1 in producing EDNs and NO. To test this hypothesis, we investigated 1) the expression and localization of GPER1 in bovine oviductal tissues and 2) rapid effects of E2 via GPER1 on EDN1, EDN2 and inducible NO synthase (iNOS) expression in cultured bovine oviductal isthmic epithelial cells. GPER1 was observed in the oviductal epithelium, stroma and smooth muscle, and its expression was highest in the isthmus. Short-term treatments (≤1 hr) of E2 increased EDN2 mRNA expression in the isthmic epithelial cells, although E2 did not affect EDN1 and iNOS mRNA expressions. Results of GPER1-selective agonist G-1 and GPER1-selective antagonist G-15 treatments revealed acute stimulation by E2, which is mediated via GPER1. The overall findings suggested that E2 in follicular fluid rapidly stimulates EDN2 expression via GPER1 in the isthmic epithelial cells. Follicular fluid may play a role in retention of the ovulated oocyte in the end of ampulla by contracting the isthmus for successful fertilization.

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Regulation of bovine oviductal NO synthesis by follicular steroids and prostaglandins

Cited by 10 publications

References 58 publications

Oviduct: roles in fertilization and early embryo development

Oviduct: roles in fertilization and early embryo development

Roles of EDNs in regulating oviductal NO synthesis and smooth muscle motility in cows

Acute stimulation of a smooth muscle constrictor by oestradiol‐17β via GPER1 in bovine oviducts

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