Regulation of biosynthesis of vermiculin and vermistatin inPenicillium vermiculatum

Adamcová, Jana; Proksa, B.; Fuska, J.

doi:10.1007/bf02814580

Cited by 5 publications

(2 citation statements)

References 5 publications

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“…These compounds were not believed to be biosynthetically related due to their structural dissimilarity (Hara et al, 1989). For vermiculin and vermistatin, also structurally different compounds produced by unrelated pathways, sucrose, cornsteep liquor and lactic acid favored vermiculin, while glucose in the absence of cornsteep liquor favored vermistatin (Adamcova et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Secondary metabolite scale‐up to minimize homolog impurity levels

Junker¹,

Reddy²,

Olewinski³

et al. 1998

Biotechnol. Bioeng.

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A mutant strain of Streptomyces hygroscopicus was found to produce up to 9.0 units/L of an immunoregulant precursor, immunomycin, with up to 3.5% of a lower homolog impurity under either dual fed-batch or batch conditions. Glycerol and valine were key nutrients influencing productivity and impurity levels. Soybean oil was successfully substituted for glycerol as a carbon source to minimize shot additions to batch culture. The remainder of the production medium was composed largely of defined components with the exception of yeast extract.Valine limitation increased lower homolog formation while decreasing higher homolog formation; excess valine decreased lower homolog formation below 2-3% while increasing higher homolog formation. Higher homolog formation in the presence of valine seemed to be slower than lower homolog formation in the absence of valine. Valine was believed to be the major butyrate precursor; consequently its availability influenced the impurity profile. A preliminary cost analysis suggests that elimination of added valine from the cultivation and replacement of glycerol with soybean oil can result in a 6.6-fold reduction in media costs relative to the original fed-batch process.

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Section: Introductionmentioning

confidence: 99%

Secondary metabolite scale‐up to minimize homolog impurity levels

Junker¹,

Reddy²,

Olewinski³

et al. 1998

Biotechnol. Bioeng.

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show abstract

“…However, specific assays carried out for vermistatin have shown that the nitrogen source and the availability of some organic acids, such as malate and succinate, may notably influence the yield [ 30 , 31 ], which otherwise basically depends on duration of culturing. In most cases cultures are maintained for three weeks or longer, but available data are not sufficient to indicate the most appropriate length of the fermentation cycle.…”

Section: Methodology For Production and Extraction Of Funicone-relatementioning

confidence: 99%

Occurence and Bioactivities of Funicone-Related Compounds

Nicoletti¹,

Manzo

Ciavatta

2009

IJMS

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Studies on production of secondary metabolites by fungi have received a substantial boost lately, particularly with reference to applications of their biological properties in human medicine. Funicones represent a series of related compounds for which there is accumulating evidence supporting their possible use as pharmaceuticals. This paper provides a review on the current status of knowledge on these fungal extrolites, with special reference to aspects concerning their molecular structures and biological activities.

show abstract

Secondary metabolite scale-up to minimize homolog impurity levels

Junker

Reddy

Olewinski

et al. 1998

Biotechnol. Bioeng.

View full text Add to dashboard Cite

A mutant strain of Streptomyces hygroscopicus was found to produce up to 9.0 units/L of an immunoregulant precursor, immunomycin, with up to 3.5% of a lower homolog impurity under either dual fed‐batch or batch conditions. Glycerol and valine were key nutrients influencing productivity and impurity levels. Soybean oil was successfully substituted for glycerol as a carbon source to minimize shot additions to batch culture. The remainder of the production medium was composed largely of defined components with the exception of yeast extract. Valine limitation increased lower homolog formation while decreasing higher homolog formation; excess valine decreased lower homolog formation below 2–3% while increasing higher homolog formation. Higher homolog formation in the presence of valine seemed to be slower than lower homolog formation in the absence of valine. Valine was believed to be the major butyrate precursor; consequently its availability influenced the impurity profile. A preliminary cost analysis suggests that elimination of added valine from the cultivation and replacement of glycerol with soybean oil can result in a 6.6‐fold reduction in media costs relative to the original fed‐batch process. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:595–604, 1998.

show abstract

Regulation of biosynthesis of vermiculin and vermistatin inPenicillium vermiculatum

Cited by 5 publications

References 5 publications

Secondary metabolite scale‐up to minimize homolog impurity levels

Secondary metabolite scale‐up to minimize homolog impurity levels

Occurence and Bioactivities of Funicone-Related Compounds

Secondary metabolite scale-up to minimize homolog impurity levels

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