Regulation of biosynthesis of thiolutin and aureothricin inStreptomyces kasugaensis

Abstract

L-Methionine and DL-ethionine decreased production of thiolutin and aureothricin in Streptomyces kasugaensis. In the presence of L-methionine the culture also produced 3-methylthioacrylic acid, 3-methylthiopropionic acid and 3,6-bis-(2-methylthioethyl)-2,5-dioxopiperazine. Production of the metabolites depended on the concentration of L-methionine in the medium.

Effect of amino acids containing sulfur on dithiolopyrrolone antibiotic productions by Saccharothrix algeriensis NRRL B-24137

“…The later amino acids supported the growth rate but not antibiotic productions. These coincide with Šturdíková et al (1990) which reported that the effect of methionine and ethionine on thiolutin and aureothricin productions by S. kasugaensis showed the same inhibitory effects. Many authors observed the negative effect of methionine on secondary metabolites biosynthesis such as ochratoxin A (Lisker and Paster 1983).…”

“…We found that the addition of 10 mmol l −1 cysteine or cystine exhibited a negative effect on thiolutin production. Šturdíková et al (1990) conducted a similar study on thiolutin and aureothricin productions using S. kasugaensis . They showed that the addition of cysteine to the culture medium at 0·1% (equivalent of 8·25 mmol l −1 ) did not affect the yield of thiolutin and aureothricin.…”

“…Dithiolopyrrolone class of antibiotics has a broad‐spectrum activity against a variety of micro‐organisms including gram‐positive and gram‐negative bacteria, yeasts, ameboid parasites and phytopathogenic fungi (Celmer and Solomons 1955; Šturdíková et al 1990; Lamari et al 2002a). This class of antibiotic also exhibited protozoicidal, larvicidal and insecticidal activities which suppressed the growth of Coccidioides mitis (Seneca et al 1952; Šturdíková et al 1990; Webster et al 2002). Arnold et al (1995) of the US National Cancer Institute postulated that dithiolopyrrolone derivatives have a chemopreventive activity in preventing the initiation of cell transformation from premalignant to malignant cancer in the rat tracheal epithelial cells.…”

Influence on dithiolopyrrolone antibiotic production by organic acids in Saccharothrix algeriensis NRRL B-24137

“…This www.elsevier.com/locate/procbio Process Biochemistry 42 (2007) [925][926][927][928][929][930][931][932][933] class of antibiotics exhibits protozoicidal, larvicidal and insecticidal activities [5,7,8]. Dithiolopyrrolones have also been reported to have anticancer activity [9].…”

“…The dithiolopyrrolones are effective against various microorganisms including Gram-positive and -negative bacteria, yeasts, filamentous fungi and ameboid parasites [5,7]. This www.elsevier.com/locate/procbio Process Biochemistry 42 (2007) [925][926][927][928][929][930][931][932][933] class of antibiotics exhibits protozoicidal, larvicidal and insecticidal activities [5,7,8].…”