Regulation of Bcl-2 family molecules and activation of caspase cascade involved in gypenosides-induced apoptosis in human hepatoma cells

Wang, Qwa Fun; Chen, Jung Chou; Hsieh, Shur Jong; Cheng, Chi Chih; Hsu, Shih-Kuang

doi:10.1016/s0304-3835(01)00828-x

Cited by 74 publications

(66 citation statements)

References 32 publications

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“…The level of Bak is reduced or even non-detectable in HCC cells (33)(34)(35). The pro-apoptotic role of Bak in HCC cells is further supported by studies in which different agents induced apoptosis in HCC cells by stimulating Bak expression (36,37). In accordance with these findings, our results demonstrated that AEA induced apoptosis via the upregulation of Bak in Huh7 cells.…”

Section: Discussionsupporting

confidence: 88%

Anti-proliferative effects of anandamide in human hepatocellular carcinoma cells

Xie

Liu

et al. 2012

Oncology Letters

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Abstract. In our previous study, we reported that the cannabinoid receptors CB1 and CB2 are overexpressed in human hepatocellular carcinoma (HCC) tissues. Recently, the antitumor potential of the endogenous cannabinoid anandamide (AEA) has also been addressed. The present study was conducted to investigate the anti-proliferative effects of AEA in HCC cells. The human HCC cell line Huh7 was used. Cell proliferation was measured by MTT assay and flow cytometry. Apoptotic analysis was investigated by TUNEL assay. Real-time PCR and western blot analysis were used to analyze the expression of relevant molecules. The results of this study demonstrated that AEA inhibited the proliferation of Huh7 cells, resulted in G1 cell cycle arrest and induced apoptosis. Furthermore, downregulation of CDK4 and upregulation of p21 and Bak by AEA were observed. This study defines the anti-proliferative effects of anandamide in HCC cells and suggests that AEA has therapeutic potential in the management of HCC patients.

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Section: Discussionsupporting

confidence: 88%

Anti-proliferative effects of anandamide in human hepatocellular carcinoma cells

Xie

Liu

et al. 2012

Oncology Letters

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“…c; ↑caspase-3, -9, -8 0.1-400 mg/mL Wang et al [98] IH-901 Conferred antiproliferative effects against HepG2 cells ↑apoptosis; ↑caspase-3, -8, -9; ↑ PARP; ↑cyt. c 10-60 μmol/L Oh et al [99] Inhibited the growth of SMMC7721 cells ↑apoptosis; ⊥G0/G1; ↑Bax; ↑p53; ↑cyt.…”

Section: +mentioning

confidence: 98%

“…c, caspase-3, -9 and -8 [98] . IH-901, a novel metabolite of the ginseng saponin, exhibited cytotoxicity against HepG2 cells with simultaneous induction of apoptosis via mitochondrial-mediated pathway, which resulted in the activation of caspase-9 and subsequent mitochondrial pathway activation.…”

Section: Lupeolmentioning

confidence: 99%

Terpenoids as potential chemopreventive and therapeutic agents in liver cancer

Thoppil

Bishayee²

2011

WJH

267

143

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Despite significant advances in medicine, liver cancer, predominantly hepatocellular carcinoma remains a major cause of death in the United States as well as the rest of the world. As limited treatment options are currently available to patients with liver cancer, novel preventive control and effective therapeutic approaches are considered to be reasonable and decisive measures to combat this disease. Several naturally occurring dietary and non-dietary phytochemicals have shown enormous potential in the prevention and treatment of several cancers, especially those of the gastrointestinal tract. Terpenoids, the largest group of phytochemicals, traditionally used for medicinal purposes in India and China, are currently being explored as anticancer agents in clinical trials. Terpenoids (also called "isoprenoids") are secondary metabolites occurring in most organisms, particularly plants. More than 40 000 individual terpenoids are known to exist in nature with new compounds being discovered every year. A large number of terpenoids exhibit cytotoxicity against a variety of tumor cells and cancer preventive as well as anticancer efficacy in preclinical animal models. This review critically examines the potential role of naturally occurring terpenoids, from diverse origins, in the chemoprevention and treatment of liver tumors. Both in vitro and in vivo effects of these agents and related cellular and molecular mechanisms are highlighted. Potential challenges and future directions involved in the advancement of these promising natural compounds in the chemoprevention and therapy of human liver cancer are also discussed.

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“…Extracts of G. pentaphyllum and/or isolated gypenosides have been reported to exert a variety of effects, such as the inhibition of inflammation (7), anti-oxidant (6) and lipid-lowering effects (23), as well as anti-carcinogenic (9) and proapoptotic properties (24). In previous investigations of G. pentaphyllum, Hu et al (25) have isolated dammarane-type glycosides structurally related to the ginseng saponins.…”

Section: Fig 2 Separation Of Optical Isomers Of the Active Compoundmentioning

confidence: 99%

A Novel Insulin-releasing Substance, Phanoside, from the Plant Gynostemma pentaphyllum

Norberg

Hoa

Лиепиньш

et al. 2004

Journal of Biological Chemistry

101

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Extracts from Gynostemma pentaphyllum Makino (Cucurbitaceae), a Southeast Asian herb, has been reported to affect numerous activities resulting in antitumor, cholesterol-lowering, immunopotentiating, antioxidant, and hypoglycemic effects. We have isolated one active compound by ethanol extraction, distribution in n-butyl alcohol/water, solid phase extraction/separation, and several rounds of reverse phase high pressure liquid chromatography. We have shown by NMR and mass spectrometry that this active compound is a novel saponin, a gypenoside, which we have named phanoside Phanoside is a dammarane-type saponin, and four stereoisomers differing in configurations at positions 21 and 23 were identified, each of which were found to stimulate insulin release from isolated rat pancreatic islets. We have also found that the stereoisomers are interconvertible. Dose-dependent insulin-releasing activities at 3.3 and 16.7 mM glucose levels were determined for the racemic mixture containing all four stereoisomers. Phanoside at 500 M stimulates insulin release in vitro 10-fold at 3.3 mM glucose and potentiates the release almost 4-fold at 16.7 mM glucose. At these glucose levels, 2 M glibenclamide stimulates insulin release only 2-fold. Interestingly, ␤-cell sensitivity to phanoside is higher at 16.7 mM than at 3.3 mM glucose, although insulin responses were significantly increased by phanoside below 125 M only at high glucose levels. Also when given orally to rats, phanoside (40 and 80 mg/ml) improved glucose tolerance and enhanced plasma insulin levels at hyperglycemia.

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Regulation of Bcl-2 family molecules and activation of caspase cascade involved in gypenosides-induced apoptosis in human hepatoma cells

Cited by 74 publications

References 32 publications

Anti-proliferative effects of anandamide in human hepatocellular carcinoma cells

Anti-proliferative effects of anandamide in human hepatocellular carcinoma cells

Terpenoids as potential chemopreventive and therapeutic agents in liver cancer

A Novel Insulin-releasing Substance, Phanoside, from the Plant Gynostemma pentaphyllum

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