Regulation of Arabidopsis MAPKKK18 by ABI1 and SnRK2, components of the ABA signaling pathway

Tajdel, Małgorzata; Mituła, Filip; Ludwików, Agnieszka

doi:10.1080/15592324.2016.1139277

Cited by 29 publications

(23 citation statements)

References 16 publications

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“…At a transcriptional level, the M3K18 promoter demonstrated higher promoter efficiency following stimulation with ABA in plant guard cells. These results indicated that, MAP3K18 interacts directly with key components of the ABA signaling module, such as SnRK2.6 kinase [19] and PP2C phosphatase ABI1 [17]. ABA engages PYR/PYL ( Figure 1) and prevents the degradation of MAP3K18, which ensures the stability and activation of downstream kinase processes to trigger stress signaling modules [17].…”

Section: Mitogen Activated Protein Kinase (Mapk) Signaling Pathwaymentioning

confidence: 93%

Insights into Drought Stress Signaling in Plants and the Molecular Genetic Basis of Cotton Drought Tolerance

Mahmood

Khalid

Abdullah

et al. 2019

Cells

235

141

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Drought stress restricts plant growth and development by altering metabolic activity and biological functions. However, plants have evolved several cellular and molecular mechanisms to overcome drought stress. Drought tolerance is a multiplex trait involving the activation of signaling mechanisms and differentially expressed molecular responses. Broadly, drought tolerance comprises two steps: stress sensing/signaling and activation of various parallel stress responses (including physiological, molecular, and biochemical mechanisms) in plants. At the cellular level, drought induces oxidative stress by overproduction of reactive oxygen species (ROS), ultimately causing the cell membrane to rupture and stimulating various stress signaling pathways (ROS, mitogen-activated-protein-kinase, Ca2+, and hormone-mediated signaling). Drought-induced transcription factors activation and abscisic acid concentration co-ordinate the stress signaling and responses in cotton. The key responses against drought stress, are root development, stomatal closure, photosynthesis, hormone production, and ROS scavenging. The genetic basis, quantitative trait loci and genes of cotton drought tolerance are presented as examples of genetic resources in plants. Sustainable genetic improvements could be achieved through functional genomic approaches and genome modification techniques such as the CRISPR/Cas9 system aid the characterization of genes, sorted out from stress-related candidate single nucleotide polymorphisms, quantitative trait loci, and genes. Exploration of the genetic basis for superior candidate genes linked to stress physiology can be facilitated by integrated functional genomic approaches. We propose a third-generation sequencing approach coupled with genome-wide studies and functional genomic tools, including a comparative sequenced data (transcriptomics, proteomics, and epigenomic) analysis, which offer a platform to identify and characterize novel genes. This will provide information for better understanding the complex stress cellular biology of plants.

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Section: Mitogen Activated Protein Kinase (Mapk) Signaling Pathwaymentioning

confidence: 93%

Insights into Drought Stress Signaling in Plants and the Molecular Genetic Basis of Cotton Drought Tolerance

Mahmood

Khalid

Abdullah

et al. 2019

Cells

235

141

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“…Consistent with this, studies of the transcriptional regulation of the MKKK18 promotor revealed high promoter activity following ABA stimulation in guard cells ( Mitula et al, 2015 ). Importantly, experimental results indicate that MAPKKK18 interacts directly with two of the key proteins of the ABA core signaling module, PP2C phosphatase ABI1 ( Mitula et al, 2015 ) and kinase SnRK2.6 ( Tajdel et al, 2016 ). ABI1, in the absence of ABA, not only inhibits MAPKKK18 kinase activity by dephosphorylation, but also targets MAPKKK18 for degradation by the ubiquitin-proteasome pathway (UPS) ( Ludwików, 2015 ; Mitula et al, 2015 ).…”

Section: Mapk Cascades In Abscisic Acid Signalingmentioning

confidence: 99%

Mitogen-Activated Protein Kinase Cascades in Plant Hormone Signaling

et al. 2018

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Mitogen-activated protein kinase (MAPK) modules play key roles in the transduction of environmental and developmental signals through phosphorylation of downstream signaling targets, including other kinases, enzymes, cytoskeletal proteins or transcription factors, in all eukaryotic cells. A typical MAPK cascade consists of at least three sequentially acting serine/threonine kinases, a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK) and finally, the MAP kinase (MAPK) itself, with each phosphorylating, and hence activating, the next kinase in the cascade. Recent advances in our understanding of hormone signaling pathways have led to the discovery of new regulatory systems. In particular, this research has revealed the emerging role of crosstalk between the protein components of various signaling pathways and the involvement of this crosstalk in multiple cellular processes. Here we provide an overview of current models and mechanisms of hormone signaling with a special emphasis on the role of MAPKs in cell signaling networks.One-sentence summary: In this review we highlight the mechanisms of crosstalk between MAPK cascades and plant hormone signaling pathways and summarize recent findings on MAPK regulation and function in various cellular processes.

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“…The ABA signaling pathway plays an essential role in regulating plant growth and tolerance to stressful conditions like drought. The coreceptor ABI1 is not only a part of a negative feedback loop within the ABA signaling pathway, but also acts as a hub for hormone cross-talk and is subject to multilevel regulation (17,19,38,39). Our results demonstrate that the ubiquitination of ABI1, mediated by the UBC27-AIRP3 ubiquitination complex, is crucial for ABA responses.…”

Section: Discussionmentioning

confidence: 73%

The UBC27–AIRP3 ubiquitination complex modulates ABA signaling by promoting the degradation of ABI1 in Arabidopsis

Pan

Lin

Yang

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Abscisic acid (ABA) is the key phytohormone in plant drought tolerance and stress adaptation. The clade A protein phosphatase 2Cs (PP2Cs) like ABI1 (ABA-INSENSITIVE 1) work as coreceptors of ABA and regulate multiple ABA responses. Ubiquitination of ABI1 has been proven to play important regulatory roles in ABA signaling. However, the specific ubiquitin conjugating enzyme (E2) involved is unknown. Here, we report that UBC27 is an active E2 that positively regulates ABA signaling and drought tolerance. UBC27 forms the E2-E3 pair with the drought regulator RING E3 ligase AIRP3. Both UBC27 and AIRP3 interact with ABI1 and affect the ubiquitination and degradation of ABI1. ABA activates the expression of UBC27, inhibits the proteasome degradation of UBC27, and enhances the interaction between UBC27 and ABI1 to increase its activity. These findings uncover a regulatory mechanism in ABA signaling and drought response and provide a further understanding of the plant ubiquitination system and ABA signaling pathway.

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Regulation of Arabidopsis MAPKKK18 by ABI1 and SnRK2, components of the ABA signaling pathway

Cited by 29 publications

References 16 publications

Insights into Drought Stress Signaling in Plants and the Molecular Genetic Basis of Cotton Drought Tolerance

Insights into Drought Stress Signaling in Plants and the Molecular Genetic Basis of Cotton Drought Tolerance

Mitogen-Activated Protein Kinase Cascades in Plant Hormone Signaling

The UBC27–AIRP3 ubiquitination complex modulates ABA signaling by promoting the degradation of ABI1 in Arabidopsis

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