Regulation of antioxidant mechanisms by AtDREB1A improves soil-moisture deficit stress tolerance in transgenic peanut (Arachis hypogaea L.)

Abstract

The present study evaluated the soil-moisture deficit stress tolerance of AtDREB1A transgenic peanut lines during reproductive stages using lysimetric system under controlled glasshouse conditions. The antioxidant activities of AtDREB1A transgenic lines were measured by biochemical assays. The transgenic peanut lines recorded significantly lower accumulation of malondialdehyde and hydrogen peroxide than the wild-type. Whereas, specific activity of catalase, guaiacol peroxidase, ascorbate peroxidase, glutathione reductase and ascorbic acid were found to be significantly higher in transgenic lines than in the wild-type line under drought stress. The results showed that the transgenic lines expressed lower oxidative damage than wild-type and could protect themselves from the elevated levels of reactive oxygen species under drought stress. This could be attributed to the regulation of various stress-inducible genes by AtDREB1A transcription factor. Improved photosynthetic and growth parameters were also recorded in transgenic lines over wild-type under drought stress. Improved physio-biochemical mechanisms in transgenic peanut lines might have resulted in improved growth-related traits as significant correlations were observed between physio-biochemical parameters and growth-related traits under drought stress. The potential target genes of AtDREB1A transcription factor in transgenic peanut lines during drought stress were identified, which helped in understanding the molecular mechanisms of DREB-regulated stress responses. The transgenic line D6 reported the best physio-biochemical mechanisms and growth-related parameters under drought stress over other transgenic lines and wild-type, suggesting it may be used to develop high yielding and terminal drought-tolerant peanut varieties.

Diversity in Phytochemical Composition, Antioxidant Capacities, and Nutrient Contents Among Mungbean and Lentil Microgreens When Grown at Plain-Altitude Region (Delhi) and High-Altitude Region (Leh-Ladakh), India

“…Interestingly, TAA was also found significantly correlated with FRAP ( r = 0.501; P <0.05) and total carotenoid content ( r = 0.704; P <0.01) in lentil microgreens, indicating their role in the total AoA of lentil microgreens. Such correlation studies can give a more precise idea of the parameter(s) contributing to the AoA (Patel et al, 2016 ; Bhalani et al, 2019 ; Devi et al, 2019 ).…”

Morphological, Molecular, and Biochemical Characterization of a Unique Lentil (Lens culinaris Medik.) Genotype Showing Seed-Coat Color Anomalies Due to Altered Anthocyanin Pathway

“…Similarly, FRAP was found to be significantly correlated with TAA ( p ≤ 0.05) in lentil microgreens [ 56 ] indicating their role in the AoA of lentil seeds, sprouts, and microgreens. Thus, correlation studies have provided a clear understanding of the parameter(s) contributing to the total AoA [ 73 , 74 , 75 ]. Proteins are the main energy source for lentil seeds, and the negative correlation between total protein content and all other studied parameters indicates that the protein is being used to produce various types of AoA in the sprouts and seedlings.…”

Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

“…Further, since RD29a is a stress-induced gene, it could promote DREB1a expression sustainably during stress conditions. RD29a promoter induced DREB1a transcripts to 2 -10-fold higher under salinity, cold, and drought conditions in Arabidopsis, rice, and peanut (Latha et al, 2019;Oh et al, 2005;Sarkar et al, 2016), and transgenic expression of RD29a:DREB1a conferred tolerance to salinity, drought, and cold stress in diverse plant species including rice (Datta et al, 2012;Ganguly et al, 2020;Geda et al, 2019;Latha et al, 2019;Muthurajan et al, 2021;Oh et al, 2005;Ravikumar et al, 2014;), peanuts (Bhalani et al, 2019;Bhatnagar-Mathur et al, 2007;Devi et al, 2011;Sarkar et al, 2016) chickpea (Anbazhagan et al, 2015), soybean (de Paiva et al, 2014), chrysanthemum (Hong et al, 2006), potato (Behnam et al, 2007;Shimazaki et al, 2016) and Salvia sp. (Wei et al, 2016).…”