Regulation of ANKRD9 expression by lipid metabolic perturbations

Wang, Xiaofei; Newkirk, Robert F.; Carré, Wilfrid; Ghose, Purnima; Igobudia, Barry; Townsel, James G.; Cogburn, Larry A.

doi:10.5483/bmbrep.2009.42.9.568

Cited by 13 publications

(16 citation statements)

References 27 publications

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“…An EST (GenBank accession CF255001) derived from CNV locus 13 (chr4:88,954,181-88,987,642) was used to design primers for transcript level analysis. RT-qPCR was carried out as described previously [62] with slight modifications using QuantiTect SYBR Green RT-PCR kit (Qiagen). To use iCycler equipment with the RT-PCR kit, fluorescein (Bio-Rad) was added to a final concentration of 10 nM.…”

Section: Methodsmentioning

confidence: 99%

An initial map of chromosomal segmental copy number variations in the chicken

et al. 2010

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BackgroundChromosomal segmental copy number variation (CNV) has been recently recognized as a very important source of genetic variability. Some CNV loci involve genes or conserved regulatory elements. Compelling evidence indicates that CNVs impact genome functions. The chicken is a very important farm animal species which has also served as a model for biological and biomedical research for hundreds of years. A map of CNVs in chickens could facilitate the identification of chromosomal regions that segregate for important agricultural and disease phenotypes.ResultsNinety six CNVs were identified in three lines of chickens (Cornish Rock broiler, Leghorn and Rhode Island Red) using whole genome tiling array. These CNVs encompass 16 Mb (1.3%) of the chicken genome. Twenty six CNVs were found in two or more animals. Whereas most small sized CNVs reside in none coding sequences, larger CNV regions involve genes (for example prolactin receptor, aldose reductase and zinc finger proteins). These results suggest that chicken CNVs potentially affect agricultural or disease related traits.ConclusionAn initial map of CNVs for the chicken has been described. Although chicken genome is approximately one third the size of a typical mammalian genome, the pattern of chicken CNVs is similar to that of mammals. The number of CNVs detected per individual was also similar to that found in dogs, mice, rats and macaques. A map of chicken CNVs provides new information on genetic variations for the understanding of important agricultural traits and disease.

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Section: Methodsmentioning

confidence: 99%

An initial map of chromosomal segmental copy number variations in the chicken

et al. 2010

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“…The RNA was pretreated with DNase I (RNase free) to eliminate DNA contamination. mRNA preparation and reverse transcription reaction were performed using a cDNA PCR Library Kit and cDNA Synthesis Kit according to manufacturer's protocol (TaKaRa) described previously (22).…”

Section: Construction Of Cdna Library Of Human Embryo Heartmentioning

confidence: 99%

“…GAPDH was amplified as the control. Plasmid construction: The L8G5-Luc, pSRE-Luc and pAP-1-Luc constructs used were generated previously in the lab (22). An EGFP-C1-ZNF552 expression plasmid was constructed by inserting ZNF552 cDNA which had cloned into pUCm-T vector into pEGFP-C1 (Clontech, San Jose, USA) vector at the downstream of EGFP with HindIII and SalI sites.…”

Section: Identification Of Znf552 Mrna In Human Embryo Tissues By Rt-pcrmentioning

confidence: 99%

“…To generate a fusion protein of KRAB, KRAB -1C2H2, 1C2H2, 1C2H2-6C2H2 and 6C2H2 fragments with GAL4 or FLAG tag, the five fragments were amplified by PCR with primers ZNF552-S1/ZNF552-A2, ZNF552-S1/ZNF552-A3, ZNF552-S2/ ZNF552-A3, ZNF552-S2/ZNF552-A1 and ZNF552-S3/ ZNF552-A1 (Supplement Table 2), respectively, and then subcloned to the BamHI and SalI sites of the pCMV-BD and pCMV-Tag2B. Cell culture, transient transfection, and subcellular localization analysis: COS-7 cells used in all studies were maintained and passaged according to standard methods described previously (14,22), and transfected with pEGFP-C1-ZNF552 plasmid DNA using Lipofectamine 2000 (Invitrogen, Carlsbad, USA) according to the manufacturer's instructions. Forty-eight hours after the transfection, the localization of the fusion protein (EGFP-ZNF552) was visualized with epifluorescence microscope after labeling with DAPI for nuclei.…”

Section: Identification Of Znf552 Mrna In Human Embryo Tissues By Rt-pcrmentioning

confidence: 99%

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ZNF552, a novel human KRAB/C2H2 zinc finger protein, inhibits AP-1- and SRE-mediated transcriptional activity

Deng

Liu²,

Fan³

et al. 2010

BMB Reports

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In this study, we report the identification and characterization of a novel C2H2 zinc finger protein, ZNF552, from a human embryonic heart cDNA library. ZNF552 is composed of three exons and two introns and maps to chromosome 19q13.43. The cDNA of ZNF552 is 2.3 kb, encoding 407 amino acids with an amino-terminal KRAB domain and seven carboxyl-terminal C2H2 zinc finger motifs in the nucleus and cytoplasm. Northern blotting analysis indicated that a 2.3 kb transcript specific for ZNF552 was expressed in liver, lung, spleen, testis and kidney, especially with a higher level in the lung and testis in human adult tissues. Reporter gene assays showed that ZNF552 was a transcriptional repressor, and overexpression of ZNF552 in the COS-7 cells inhibited the transcriptional activities of AP-1 and SRE, which could be relieved through RNAi analysis. Deletion studies showed that the KRAB domain of ZNF552 may be involved in this inhibition. [BMB reports 2010; 43(3) : 193-198]

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“…COS-7 cells used in all studies were maintained and passaged according to standard methods described previously (25). Transient transfections of cells with the reporter plasmid (pFRLuc, p21-Luc, p53-Luc), pCMV-LacZ and the indicated expression vectors were carried out with Lipofectamine 2000 (Invitrogen).…”

Section: Luciferase Assaysmentioning

confidence: 99%

Synergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21

Deng¹,

Li²,

Fan³

et al. 2010

BMB Reports

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LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes αB-crystallin. Co-immunoprecipitation and GST pull-down assays showed that LBH interacts with αB-crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, αB-crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or αB-crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both αB-crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and αB-crystallin may inhibit synergistically the transcriptional regulation of p53 and p21. [BMB reports 2010; 43(6): 432-437]

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Regulation of ANKRD9 expression by lipid metabolic perturbations

Abstract: Fatty acid oxidation (FAO) defects cause abnormal lipid accumulation in various tissues

Cited by 13 publications

References 27 publications

An initial map of chromosomal segmental copy number variations in the chicken

An initial map of chromosomal segmental copy number variations in the chicken

ZNF552, a novel human KRAB/C2H2 zinc finger protein, inhibits AP-1- and SRE-mediated transcriptional activity

Synergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21

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