Regulation of amino acid metabolism as a defensive strategy in the brain of three freshwater teleosts in response to high environmental ammonia exposure

Sinha, Amit Kumar; Giblen, Terri; AbdElgawad, Hamada; Rop, Michelle De; Asard, Han; Blust, Ronny; Boeck, Gudrun De

doi:10.1016/j.aquatox.2013.01.003

Cited by 100 publications

(58 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The activity of GS in trout, carp and goldfish showed a tendency to increase in response to high environmental ammonia (Sinha et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…The increase in serum ammonia concentrations by exposure to ambient ammonia is consistent with previous studies (Vedel, Korsgaard & Jensen ). Trout, carp and goldfish exposed to high environmental ammonia received a net influx of ammonia from the medium into the tissue, which was evident from a dramatic increase in ammonia concentrations (Sinha, Giblen, AbdElgawad, De Rop, Asard, Blust & De Boeck ). The mechanism underlying these changes is thought to be a detoxification of ammonia in a reaction catalysed by GS (Haghighat & McCandless ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of dietary α‐ketoglutarate supplementation on the antioxidant defense system and HSP 70 and HSP 90 gene expression of hybrid sturgeon A cipenser schrenckii ♀ × A . baerii ♂ exposed to ammonia‐N stress

Wang

et al. 2016

Aquac Res

View full text Add to dashboard Cite

This study was conducted to determine the effects of dietary a-ketoglutarate (AKG) supplementation on the antioxidant defense system and gene expression of heat shock protein (HSP) 70 and HSP 90 in hybrid sturgeons Acipenser schrenckii ♀ 9 A. baerii ♂ exposed to ammonia-N stress. A 2 9 3 factorial experiment was arranged, in which each diet (0%, 1% AKG) was randomly assigned to 0.25 (control) 5 and 10 mg L À1 ammonia-N groups with three replicate aquaria for each 72 h. The 10 mg L À1 ammonia-N significantly increased serum ammonia concentrations and intestinal Gln concentrations and GS activity compared with the 0.25 or 5 mg L À1 ammonia-N groups. The intestinal Gln concentration and GS activity increased, and the serum ammonia concentration decreased, in fish given dietary supplementation of 1.0% AKG compared with fish given diets without AKG. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity in serum, gills and intestines decreased when fish were exposed to 5 or 10 mg L À1 ammonia-N, and their activity increased in fish given diets with 1% AKG. Catalase in the serum and gills decreased when fish were exposed to 5 or 10 mg L À1 ammonia-N and increased in fish given diets with 1% AKG. The 10 mg L À1 ammonia-N or 1% AKG supplementation increased HSP 70 and HSP 90 gene expression in the liver. The increased activity of antioxidant enzymes, and increased HSP 70 and HSP 90 gene expression in fish fed diets containing 1% AKG suggested higher tolerance to ammonia-N stress.

show abstract

“…The activity of GS in trout, carp and goldfish showed a tendency to increase in response to high environmental ammonia (Sinha et al . ).…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Effects of dietary α‐ketoglutarate supplementation on the antioxidant defense system and HSP 70 and HSP 90 gene expression of hybrid sturgeon A cipenser schrenckii ♀ × A . baerii ♂ exposed to ammonia‐N stress

Wang

et al. 2016

Aquac Res

View full text Add to dashboard Cite

show abstract

“…Less ammonia tolerant teleosts such as the common carp (Cyprinus carpio) and the rainbow trout (Oncorhynchus mykiss) experienced respective plasma T Amm levels of 1400 μmol L −1 and 2200 μmol L −1 during exposure to 1 mmol L − 1 T Amm in fresh water (Sinha et al, 2013a). Among the fishes with the highest reported tolerances to ammonia are the air-breathing amphibious fishes, found predominately in tropical regions.…”

Section: Pacific Hagfish Exhibit High Tolerance To Ammoniamentioning

confidence: 98%

Adaptations of a deep sea scavenger: High ammonia tolerance and active NH 4 + excretion by the Pacific hagfish ( Eptatretus stoutii )

Clifford

Goss

Wilkie

2015

Comparative Biochemistry and Physiology Part A: Molecular &

View full text Add to dashboard Cite

“…After centrifugation and filtration by Millipore micro filters (0.2 lM pore size), glycine and serine contents were determined by using a Waters Acquity UPLC-tqd system (Milford, MA, USA) equipped with a BEH amide 2.1 9 50 column (Sinha et al, 2013). After centrifugation, the supernatant was vacuum evaporated, and the pellet was resuspended in 1 ml chloroform.…”

Section: Glycine and Serinementioning

confidence: 99%

Physiological, biochemical, and genome‐wide transcriptional analysis reveals that elevated CO₂ mitigates the impact of combined heat wave and drought stress in Arabidopsis thaliana at multiple organizational levels

Zinta

AbdElgawad

Domagalska

et al. 2014

Global Change Biology

Self Cite

166

140

View full text Add to dashboard Cite

Climate changes increasingly threaten plant growth and productivity. Such changes are complex and involve multiple environmental factors, including rising CO2 levels and climate extreme events. As the molecular and physiological mechanisms underlying plant responses to realistic future climate extreme conditions are still poorly understood, a multiple organizational level analysis (i.e. eco-physiological, biochemical, and transcriptional) was performed, using Arabidopsis exposed to incremental heat wave and water deficit under ambient and elevated CO2 . The climate extreme resulted in biomass reduction, photosynthesis inhibition, and considerable increases in stress parameters. Photosynthesis was a major target as demonstrated at the physiological and transcriptional levels. In contrast, the climate extreme treatment induced a protective effect on oxidative membrane damage, most likely as a result of strongly increased lipophilic antioxidants and membrane-protecting enzymes. Elevated CO2 significantly mitigated the negative impact of a combined heat and drought, as apparent in biomass reduction, photosynthesis inhibition, chlorophyll fluorescence decline, H2 O2 production, and protein oxidation. Analysis of enzymatic and molecular antioxidants revealed that the stress-mitigating CO2 effect operates through up-regulation of antioxidant defense metabolism, as well as by reduced photorespiration resulting in lowered oxidative pressure. Therefore, exposure to future climate extreme episodes will negatively impact plant growth and production, but elevated CO2 is likely to mitigate this effect.

show abstract

Regulation of amino acid metabolism as a defensive strategy in the brain of three freshwater teleosts in response to high environmental ammonia exposure

Cited by 100 publications

References 57 publications

Effects of dietary α‐ketoglutarate supplementation on the antioxidant defense system and HSP 70 and HSP 90 gene expression of hybrid sturgeon A cipenser schrenckii ♀ × A . baerii ♂ exposed to ammonia‐N stress

Effects of dietary α‐ketoglutarate supplementation on the antioxidant defense system and HSP 70 and HSP 90 gene expression of hybrid sturgeon A cipenser schrenckii ♀ × A . baerii ♂ exposed to ammonia‐N stress

Adaptations of a deep sea scavenger: High ammonia tolerance and active NH 4 + excretion by the Pacific hagfish ( Eptatretus stoutii )

Physiological, biochemical, and genome‐wide transcriptional analysis reveals that elevated CO₂ mitigates the impact of combined heat wave and drought stress in Arabidopsis thaliana at multiple organizational levels

Contact Info

Product

Resources

About

Regulation of amino acid metabolism as a defensive strategy in the brain of three freshwater teleosts in response to high environmental ammonia exposure

Cited by 100 publications

References 57 publications

Effects of dietary α‐ketoglutarate supplementation on the antioxidant defense system and HSP 70 and HSP 90 gene expression of hybrid sturgeon A cipenser schrenckii ♀ × A . baerii ♂ exposed to ammonia‐N stress

Effects of dietary α‐ketoglutarate supplementation on the antioxidant defense system and HSP 70 and HSP 90 gene expression of hybrid sturgeon A cipenser schrenckii ♀ × A . baerii ♂ exposed to ammonia‐N stress

Adaptations of a deep sea scavenger: High ammonia tolerance and active NH 4 + excretion by the Pacific hagfish ( Eptatretus stoutii )

Physiological, biochemical, and genome‐wide transcriptional analysis reveals that elevated CO2 mitigates the impact of combined heat wave and drought stress in Arabidopsis thaliana at multiple organizational levels

Contact Info

Product

Resources

About

Physiological, biochemical, and genome‐wide transcriptional analysis reveals that elevated CO₂ mitigates the impact of combined heat wave and drought stress in Arabidopsis thaliana at multiple organizational levels