Regulation of Alpha2(I), Alpha1(III), and Alpha2(V) Collagen mRNAs by Estradiol in the Immature Rat Uterus

Frankel, Fred R.; Hsu, Charles; Myers, Jeanne C.; Lin, Emily; Lyttle, C. Richard; Komm, Barry S.; Mohn, Kenneth

doi:10.1089/dna.1.1988.7.347

Cited by 24 publications

(9 citation statements)

References 33 publications

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“…COL3A1 mRNA was abundant during proestrus (day 16 cycle), suggesting that estrogen up-regulates COL3A1 expression in ovine uterine stromal cells. This is consistent with estrogen stimulation of expression of collagen types I, III, and V in rat uteri (Frankel et al 1988) and collagen types I, III, and VI in uterine stromal cells of postmenopausal women (Iwahashi et al 1997). COL3A1 is the collagen type that increases tensile strength (van der Rest & Garrone 1991, Burgeson & Nimni 1992, Aumailley & Gayraud 1998.…”

Section: P 4 and Ifnt-regulated Genes In Ovine Uterisupporting

confidence: 69%

Progesterone and interferon tau-regulated genes in the ovine uterine endometrium: identification of periostin as a potential mediator of conceptus elongation

Ahn

Farmer²,

Bazer³

et al. 2009

Reproduction

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During early pregnancy in ruminants, progesterone (P 4 ) and interferon tau (IFNT) act on the endometrium to regulate genes hypothesized to be important for conceptus development and implantation. The present study was conducted to verify several candidate genes (actin a-2, smooth muscle, aorta (ACTA2), collagen, type III, a-1 (COL3A1), periostin (POSTN), secreted protein acidic cysteine-rich (SPARC), S100 calcium-binding protein A2 (S100A2), STAT5A and transgelin (TAGLN)) regulated by pregnancy, P 4 , and/or IFNT in the endometrium determined using a custom ovine cDNA array. S100A2 mRNA was detected primarily in endometrial epithelia and conceptuses. S100A2 mRNA increased in endometrial epithelia from days 10 to 16 in cyclic ewes and from days 10 to 14 in pregnant ewes and declined thereafter. The abundance of S100A2 mRNA was less in endometrial luminal epithelium of IFNT-infused ewes receiving P 4 . Expression of COL3A1, SPARC, ACTA2, and TAGLN was independent of pregnancy, P 4 , or IFNT. POSTN mRNA was detected primarily in compact stroma of intercaruncular and caruncular endometria, but not in the conceptus. Endometrial POSTN mRNA increased between days 12 and 14 in pregnant but not cyclic ewes, and POSTN mRNA was more abundant in uterine stroma of ewes treated with P 4 . POSTN protein was detected in uterine flushings of pregnant ewes and found to mediate attachment and stimulate migration of ovine trophectoderm cells in vitro. These results support the ideas that POSTN and S100A2 are regulated by P 4 and IFNT respectively, and that POSTN is involved in conceptus elongation during early pregnancy. Reproduction (2009) 138 813-825

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Section: P 4 and Ifnt-regulated Genes In Ovine Uterisupporting

confidence: 69%

Progesterone and interferon tau-regulated genes in the ovine uterine endometrium: identification of periostin as a potential mediator of conceptus elongation

Ahn

Farmer²,

Bazer³

et al. 2009

Reproduction

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“…Furthermore, Woessner (1979) found that injection with E 2 causes a decrease in uterine collagenase activity that is correlated with the rate of collagen breakdown. Frankel et al (1988) found that E 2 induces alpha 2 (I) and alpha 1 (III) collagen mRNAs in the immature rat uterus, and Dyer et al (1980) reported that E 2 stimulates both synthesis and degradation of collagen in the ovariectomized rat uterus. It therefore seems that the effect of estrogen on collagen metabolism in the uterus changes according to the physiological state of the animals.…”

Section: Discussionmentioning

confidence: 99%

The effects of the aromatase inhibitor fadrozole hydrochloride on fetuses and uteri in late pregnant rats

Tamada¹,

Shimizu²,

Inaba³

et al. 2004

Journal of Endocrinology

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It is well known that progesterone and estrogen are essential hormones for maintaining pregnancy in most mammals. Some specific roles of progesterone for the maintenance of pregnancy have been clarified, but the role of estrogen is not well known. This study examines the effects of the aromatase inhibitor, fadrozole hydrochloride (Fad), on fetuses, uterine physical properties and the mRNA expression of the uterine enzymes that are related to collagen metabolism during late pregnancy in rats. Continuous s.c. infusion with 300 µg/day Fad from day 14 of pregnancy (day 1=the day of sperm detection) reduced the concentration of plasma estradiol-17 (E 2 ), and did not change that of plasma progesterone, compared with controls. The treatment increased the intrauterine pressure and reduced the size and compliance of the uterine tissue framework. It also caused injuries (hematomata on the extremities) in about one-quarter of fetuses by day 20. The collagen content of the uterine ampullae was not changed by the treatment. Uterine mRNA expressions of matrix metalloproteinase-1 (MMP-1), which degrades collagens, and of lysyl oxidase (LO), which is necessary for the formation of intra-and inter-molecular cross-links of collagen, were examined by quantitative RT-PCR. The treatment with Fad had no effect on the expression of MMP-1 mRNA and increased that of LO mRNA. Daily s.c. injection with 0·2 µg E 2 restored the changes in uterine physical properties and the mRNA expression of LO caused by the Fad treatment, and prevented fetal injury, indicating that the influences of Fad treatment are due to estrogen deficiency but not to toxicological effects of Fad. These results imply that estrogen deficiency during late pregnancy in rats obstructs development of the uterine tissue framework so as to cause fetal injury. It is possible that an increase in the uterine expression of LO gene may be involved in this obstruction.

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“…The amplification by PCR was performed with the use of 1 L of cDNA and 2 L each of 2.5 mmol/L coding and noncoding oligonucleotide primers and Platinum PCR Supermix (Life Technologies). The primer sequences were as follows: TGF-␤1: coding 5Ј-ACG CCT GAG TGG CTG TCT TTT-3Ј and noncoding 5Ј-ACG TGG AGT TTG TTA TCT TTG-3Ј 18 ; ␤-actin: coding 5Ј-GAT CTG GCA CCA CAC CTT CTA-3Ј and noncoding 5Ј-GCA GGA TGG CGT GAG GGA GAG-3Ј 19 ; collagen I: coding 5Ј-CCC ACG TAG GTG TCC TAA AGT-3Ј and noncoding 5Ј-CCG TGG TGCTAAAATAATAAA-3Ј 20 ; fibronectin: coding 5Ј-ATG ATG AGG TGC ACG TGT GT-3Ј and noncoding 5Ј-GAT GGG GTC ACA TTT CCA TC-3Ј 21 ; MMP-2: coding 5Ј-GTG CTG AAG GAC ACA CTA AA-3Ј and noncoding 5Ј-TTG CCA TCC TTC TTT TCA AA-3Ј 22 ; TIMP-2: coding 5Ј-AAA CGA CAC TTA TGG CAA AC-3Ј and noncoding 5Ј-ACA GGA AGC GTC ACT TCT CT-3Ј. 23 For this semiquantitative estimation of gene expression, we performed cycle analysis for each primer pair to determine the cycle number for optimal amplification.…”

Section: Detection Of Mrna By Reverse Transcription and Polymerase Chmentioning

confidence: 99%

Anti–Transforming Growth Factor Antibody at Low but Not High Doses Limits Cyclosporine-Mediated Nephrotoxicity Without Altering Rat Cardiac Allograft Survival

et al. 2004

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Background-Long-term treatment of cardiac transplant recipients with cyclosporine results in a progressive decline in kidney function in a large number of patients. This complication is one of the most important prognostic parameters that determine the outcome of cardiac transplantation. Transforming growth factor-␤ (TGF-␤) is one of the most potent mediators of the fibrogenic effects of cyclosporine. Methods and Results-With the use of an experimental rodent model, heterotopic heart transplantation was performed, creating histocompatibility-disparate allografts. Because TGF-␤ in part mediates both the immunosuppressive and nephrotoxic effects of cyclosporine, recipients were treated with cyclosporine with and without anti-TGF-␤ antibody to determine whether anti-TGF-␤ antibody could reduce the nephrotoxic effects of cyclosporine. Intrarenal expression of TGF-␤, collagen, fibronectin, matrix metalloproteinase-2, and tissue inhibitor of metalloproteinase-2 was studied with the use of reverse transcription-polymerase chain reaction. Intrarenal expression of TGF-␤ protein was studied by immunohistochemistry and with the use of ELISA to quantify circulating levels of TGF-␤ protein in plasma. Cyclosporine-induced graft survival (immunosuppressive effect) was abrogated with a higher concentration (2.5 mg/kg) of anti-TGF-␤ antibody, whereas a lower concentration (1 mg/kg) inhibited both cyclosporine-induced expression of fibrogenic molecules and renal toxicity. Conclusions-These results provide credence to the pivotal role of TGF-␤ in immunosuppression-associated renal toxicity in recipients of cardiac transplantation. Furthermore, these findings support a potentially significant therapeutic use of optimal concentration of anti-TGF-␤ antibody to ameliorate cyclosporine-associated nephrotoxicity in cardiac transplant recipients.

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Regulation of Alpha₂(I), Alpha₁(III), and Alpha₂(V) Collagen mRNAs by Estradiol in the Immature Rat Uterus

Cited by 24 publications

References 33 publications

Progesterone and interferon tau-regulated genes in the ovine uterine endometrium: identification of periostin as a potential mediator of conceptus elongation

Progesterone and interferon tau-regulated genes in the ovine uterine endometrium: identification of periostin as a potential mediator of conceptus elongation

The effects of the aromatase inhibitor fadrozole hydrochloride on fetuses and uteri in late pregnant rats

Anti–Transforming Growth Factor Antibody at Low but Not High Doses Limits Cyclosporine-Mediated Nephrotoxicity Without Altering Rat Cardiac Allograft Survival

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